虾青素通过清除单线态氧来保护人ARPE-19视网膜色素上皮细胞免受蓝光诱导的光毒性。

IF 3.6 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Free Radical Research Pub Date : 2023-05-01 Epub Date: 2023-12-26 DOI:10.1080/10715762.2023.2277144
Mana Kitao, Ai Yamaguchi, Takuma Tomioka, Kenji Kai, Yuki Kamei, Kenji Sugimoto, Mitsugu Akagawa
{"title":"虾青素通过清除单线态氧来保护人ARPE-19视网膜色素上皮细胞免受蓝光诱导的光毒性。","authors":"Mana Kitao, Ai Yamaguchi, Takuma Tomioka, Kenji Kai, Yuki Kamei, Kenji Sugimoto, Mitsugu Akagawa","doi":"10.1080/10715762.2023.2277144","DOIUrl":null,"url":null,"abstract":"<p><p>Age-related macular degeneration (AMD) is one of an increasing number of diseases that causes irreversible impairment and loss of vision in the elderly. AMD occurs by oxidative stress-mediated apoptosis of retinal pigment epithelium cells. The onset of AMD may be positively correlated with the exposure to blue light. We screened food-derived carotenoids for cytoprotective action against blue light irradiation using human ARPE-19 retinal pigment epithelium cells. This study revealed that blue light irradiation triggered apoptosis and oxidative stress in all-<i>trans</i>-retinal (atRAL)-exposed ARPE-19 cells by generating singlet oxygen (<sup>1</sup>O<sub>2</sub>), leading to significant cell death. We found that astaxanthin, a potent anti-oxidative xanthophyll abundant in several marine organisms including microalgae, salmon, and shrimp, significantly suppresses blue light-induced apoptotic cell death of atRAL-exposed ARPE-19 cells by scavenging <sup>1</sup>O<sub>2</sub>. Mechanistic studies using the blue-light irradiated cells also demonstrated that the cytoprotective effects of astaxanthin can be attributed to scavenging of <sup>1</sup>O<sub>2</sub> directly. Our results suggest the potential value of astaxanthin as a dietary strategy to prevent blue light-induced retinal degeneration including AMD.</p>","PeriodicalId":12411,"journal":{"name":"Free Radical Research","volume":null,"pages":null},"PeriodicalIF":3.6000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Astaxanthin protects human ARPE-19 retinal pigment epithelium cells from blue light-induced phototoxicity by scavenging singlet oxygen.\",\"authors\":\"Mana Kitao, Ai Yamaguchi, Takuma Tomioka, Kenji Kai, Yuki Kamei, Kenji Sugimoto, Mitsugu Akagawa\",\"doi\":\"10.1080/10715762.2023.2277144\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Age-related macular degeneration (AMD) is one of an increasing number of diseases that causes irreversible impairment and loss of vision in the elderly. AMD occurs by oxidative stress-mediated apoptosis of retinal pigment epithelium cells. The onset of AMD may be positively correlated with the exposure to blue light. We screened food-derived carotenoids for cytoprotective action against blue light irradiation using human ARPE-19 retinal pigment epithelium cells. This study revealed that blue light irradiation triggered apoptosis and oxidative stress in all-<i>trans</i>-retinal (atRAL)-exposed ARPE-19 cells by generating singlet oxygen (<sup>1</sup>O<sub>2</sub>), leading to significant cell death. We found that astaxanthin, a potent anti-oxidative xanthophyll abundant in several marine organisms including microalgae, salmon, and shrimp, significantly suppresses blue light-induced apoptotic cell death of atRAL-exposed ARPE-19 cells by scavenging <sup>1</sup>O<sub>2</sub>. Mechanistic studies using the blue-light irradiated cells also demonstrated that the cytoprotective effects of astaxanthin can be attributed to scavenging of <sup>1</sup>O<sub>2</sub> directly. Our results suggest the potential value of astaxanthin as a dietary strategy to prevent blue light-induced retinal degeneration including AMD.</p>\",\"PeriodicalId\":12411,\"journal\":{\"name\":\"Free Radical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":3.6000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Free Radical Research\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1080/10715762.2023.2277144\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/12/26 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Free Radical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1080/10715762.2023.2277144","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/12/26 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

年龄相关性黄斑变性(AMD)是越来越多的导致老年人不可逆视力损害和丧失的疾病之一。AMD是由氧化应激介导的视网膜色素上皮细胞凋亡引起的。AMD的发病可能与蓝光照射呈正相关。我们使用人ARPE-19视网膜色素上皮细胞筛选了食物来源的类胡萝卜素对蓝光照射的细胞保护作用。这项研究表明,蓝光照射通过产生单线态氧(1O2),在全反式视网膜(atRAL)暴露的ARPE-19细胞中引发细胞凋亡和氧化应激,导致显著的细胞死亡。我们发现,虾青素是一种强效抗氧化叶黄素,在包括微藻、鲑鱼和虾在内的几种海洋生物中含量丰富,通过清除1O2,显著抑制蓝光诱导的atRAL暴露的ARPE-19细胞的凋亡。使用蓝光照射的细胞进行的机制研究也表明,虾青素的细胞保护作用可归因于直接清除1O2。我们的研究结果表明,虾青素作为一种预防蓝光诱导的视网膜变性(包括AMD)的饮食策略具有潜在价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Astaxanthin protects human ARPE-19 retinal pigment epithelium cells from blue light-induced phototoxicity by scavenging singlet oxygen.

Age-related macular degeneration (AMD) is one of an increasing number of diseases that causes irreversible impairment and loss of vision in the elderly. AMD occurs by oxidative stress-mediated apoptosis of retinal pigment epithelium cells. The onset of AMD may be positively correlated with the exposure to blue light. We screened food-derived carotenoids for cytoprotective action against blue light irradiation using human ARPE-19 retinal pigment epithelium cells. This study revealed that blue light irradiation triggered apoptosis and oxidative stress in all-trans-retinal (atRAL)-exposed ARPE-19 cells by generating singlet oxygen (1O2), leading to significant cell death. We found that astaxanthin, a potent anti-oxidative xanthophyll abundant in several marine organisms including microalgae, salmon, and shrimp, significantly suppresses blue light-induced apoptotic cell death of atRAL-exposed ARPE-19 cells by scavenging 1O2. Mechanistic studies using the blue-light irradiated cells also demonstrated that the cytoprotective effects of astaxanthin can be attributed to scavenging of 1O2 directly. Our results suggest the potential value of astaxanthin as a dietary strategy to prevent blue light-induced retinal degeneration including AMD.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Free Radical Research
Free Radical Research 生物-生化与分子生物学
CiteScore
6.70
自引率
0.00%
发文量
47
审稿时长
3 months
期刊介绍: Free Radical Research publishes high-quality research papers, hypotheses and reviews in free radicals and other reactive species in biological, clinical, environmental and other systems; redox signalling; antioxidants, including diet-derived antioxidants and other relevant aspects of human nutrition; and oxidative damage, mechanisms and measurement.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信