{"title":"单克隆IgM与克雷伯菌酸性多糖反应诱导荚膜肿胀和被动血凝","authors":"M. Harboe, J. Deverill, J. Eriksen","doi":"10.1111/J.1699-0463.1975.TB01613.X","DOIUrl":null,"url":null,"abstract":"(1) Three selected macroglobulinaemia sera, Ro, Th, and We gave highly specific precipitin reactions in gel against type 12, type 13 and type 35 acid polysaccharides of Klebsiella. The same specificity was observed in more sensitive passive haemagglutination tests. The activity was confined to the monoclonal IgM present in the sera, and all of the monoclonal IgM in each serum had the characteristic activity. (2) The monoclonal IgM proteins induced typical capsular swelling reactions of similar specificity with Klebsiella bacteria. (3) In both precipitation, passive haemagglutination and capsular swelling reactions, the IgM Ro reacted both with type 12 and type 13 polysaccharides. Absorption experiments in situ in crossed immunoelectro-phoresis indicated that the same macroglobulin molecules reacted both with type 12 and type 13 polysaccharides. This cross-reaction evident in monoclonal IgM has not been seen previously in immune antibodies'against Klebsiella type 12 and 13.","PeriodicalId":75411,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","volume":"83 1","pages":"97-105"},"PeriodicalIF":0.0000,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/J.1699-0463.1975.TB01613.X","citationCount":"2","resultStr":"{\"title\":\"CAPSULAR SWELLING AND PASSIVE HAEMAGGLUTINATION INDUCED BY MONOCLONAL IgM REACTING WITH ACID POLYSACCHARIDES OF KLEBSIELLA\",\"authors\":\"M. Harboe, J. Deverill, J. Eriksen\",\"doi\":\"10.1111/J.1699-0463.1975.TB01613.X\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"(1) Three selected macroglobulinaemia sera, Ro, Th, and We gave highly specific precipitin reactions in gel against type 12, type 13 and type 35 acid polysaccharides of Klebsiella. The same specificity was observed in more sensitive passive haemagglutination tests. The activity was confined to the monoclonal IgM present in the sera, and all of the monoclonal IgM in each serum had the characteristic activity. (2) The monoclonal IgM proteins induced typical capsular swelling reactions of similar specificity with Klebsiella bacteria. (3) In both precipitation, passive haemagglutination and capsular swelling reactions, the IgM Ro reacted both with type 12 and type 13 polysaccharides. Absorption experiments in situ in crossed immunoelectro-phoresis indicated that the same macroglobulin molecules reacted both with type 12 and type 13 polysaccharides. This cross-reaction evident in monoclonal IgM has not been seen previously in immune antibodies'against Klebsiella type 12 and 13.\",\"PeriodicalId\":75411,\"journal\":{\"name\":\"Acta pathologica et microbiologica Scandinavica. Section C, Immunology\",\"volume\":\"83 1\",\"pages\":\"97-105\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1111/J.1699-0463.1975.TB01613.X\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Acta pathologica et microbiologica Scandinavica. Section C, Immunology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1111/J.1699-0463.1975.TB01613.X\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta pathologica et microbiologica Scandinavica. Section C, Immunology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1111/J.1699-0463.1975.TB01613.X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
CAPSULAR SWELLING AND PASSIVE HAEMAGGLUTINATION INDUCED BY MONOCLONAL IgM REACTING WITH ACID POLYSACCHARIDES OF KLEBSIELLA
(1) Three selected macroglobulinaemia sera, Ro, Th, and We gave highly specific precipitin reactions in gel against type 12, type 13 and type 35 acid polysaccharides of Klebsiella. The same specificity was observed in more sensitive passive haemagglutination tests. The activity was confined to the monoclonal IgM present in the sera, and all of the monoclonal IgM in each serum had the characteristic activity. (2) The monoclonal IgM proteins induced typical capsular swelling reactions of similar specificity with Klebsiella bacteria. (3) In both precipitation, passive haemagglutination and capsular swelling reactions, the IgM Ro reacted both with type 12 and type 13 polysaccharides. Absorption experiments in situ in crossed immunoelectro-phoresis indicated that the same macroglobulin molecules reacted both with type 12 and type 13 polysaccharides. This cross-reaction evident in monoclonal IgM has not been seen previously in immune antibodies'against Klebsiella type 12 and 13.
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