OsFBN7-OsKAS I模块促进水稻叶绿体中质体红蛋白簇的形成

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-06-27 DOI:10.1111/nph.19081

Jiajia Li, Dongyan Kong, Ting Song, Zhenzhu Hu, Qiang Li, Benze Xiao, Felix Kessler, Zhengfeng Zhang, Guosheng Xie

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引用次数: 1

摘要

与类囊体膜外小叶相连的质体红蛋白(PGs)调节脂质代谢、质体发育转变和对环境刺激的反应。然而，水稻中pg核心原纤维蛋白基因OsFBN7的功能尚未被阐明。利用分子遗传学和生理化学方法，我们观察到OsFBN7的过表达促进了水稻叶绿体中PG的聚集。在水稻叶绿体中，OsFBN7与两种KAS I酶OsKAS Ia和OsKAS Ib相互作用。对OsFBN7过表达系叶绿体亚室(包括PGs)的脂质组学分析证实，PGs和叶绿体中主要膜脂质——单半乳糖二酰甘油(DAG)和单半乳糖二酰甘油(MGDG)和双半乳糖二酰甘油(DGDG)的水平均升高。此外，OsFBN7还增强了植物中OsKAS Ia/Ib的丰度及其在氧化和热胁迫下的稳定性。此外，RNA测序和实时定量反转录聚合酶链反应(qRT-PCR)分析显示，OsFBN7上调了DAG合成酶基因PAP1和MGDG合成酶基因MDG2的表达。综上所述，本研究提出了一种新的模式，即OsFBN7与叶绿体中的OsKAS Ia/Ib结合并增强其丰度和稳定性，从而调节叶绿体和PG膜脂参与PG簇的形成。

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OsFBN7–OsKAS I module promotes formation of plastoglobules clusters in rice chloroplasts

Plastoglobules (PGs) contiguous with the outer leaflets of thylakoid membranes regulate lipid metabolism, plastid developmental transitions, and responses to environmental stimuli. However, the function of OsFBN7, a PG-core fibrillin gene in rice, has not been elucidated.
Using molecular genetics and physiobiochemical approaches, we observed that OsFBN7 overexpression promoted PG clustering in rice chloroplasts.
OsFBN7 interacted with two KAS I enzymes, namely OsKAS Ia and OsKAS Ib, in rice chloroplasts. Lipidomic analysis of chloroplast subcompartments, including PGs in the OsFBN7 overexpression lines, confirmed that levels of diacylglycerol (DAG), a chloroplast lipid precursor and monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG), the main chloroplast membrane lipids, were increased in PGs and chloroplasts. Furthermore, OsFBN7 enhanced the abundances of OsKAS Ia/Ib in planta and their stability under oxidative and heat stresses. In addition, RNA sequencing and real-time quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analyses showed that the expression of the DAG synthetase gene PAP1 and MGDG synthase gene MDG2 was upregulated by OsFBN7.
In conclusion, this study proposes a new model in which OsFBN7 binds to OsKAS Ia/Ib in chloroplast and enhances their abundance and stability, thereby regulating the chloroplast and PG membrane lipids involved in the formation of PG clusters.

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.