斑马鱼胚胎作为体内试管,揭示神经发育和疾病中神经发生的细胞特异性机制

É. Samarut
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引用次数: 2

摘要

斑马鱼已经成为发育研究的首选模型,特别是在研究神经发育和疾病相关机制方面。事实上,斑马鱼提供了一个快速、方便和准确的模型来对感兴趣的基因或基因网络进行功能基因组学。最近,我们成功地利用荧光激活细胞分选(FACS)从全胚胎中纯化神经干细胞(NSCs),以便通过RNA测序分析细胞特异性转录组效应。因此,我们的工作揭示了更有可能参与我们的morpholino诱导的神经发生表型的信号通路。这种细胞纯化策略将斑马鱼提升到了一个更高的水平,因为它现在允许人们在真正的体内环境中,在基因组、转录组和蛋白质组水平上研究感兴趣的遗传条件(敲除、敲除、功能获得等)的细胞特异性效应。有了这种新的潜力,毫无疑问,斑马鱼将成为揭示癫痫、自闭症谱系障碍和精神分裂症等神经系统疾病复杂的潜在分子机制的主要模型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Zebrafish embryos as in vivo test tubes to unravel cell-specific mechanisms of neurogenesis during neurodevelopment and in diseases
ABSTRACT Zebrafish has become a model of choice for developmental studies in particular for studying neural development and related mechanisms involved in diseases. Indeed, zebrafish provides a fast, handy and accurate model to perform functional genomics on a gene or network of genes of interest. Recently, we successfully purified neural stem cells (NSCs) by fluorescence-activated cell sorting (FACS) from whole embryos in order to analyze cell-specific transcriptomic effects by RNA sequencing. As a result, our work sheds light on signaling pathways that are more likely to be involved in our morpholino-induced neurogenesis phenotype. This cell purification strategy brings zebrafish to a higher level since it now allows one to investigate cell-specific effects of a genetic condition of interest (knockout, knock-down, gain-of-function etc.) at the genomic, transcriptomic and proteomic levels in a genuine in vivo context. With this new potential, there is no doubt that zebrafish will be of a major model with which to unravel complex underlying molecular mechanisms of neurological disorders such as epilepsy, autism spectrum disorders and schizophrenia.
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