一种液泡转运体在水稻籽粒锌和镉的积累中起重要作用

IF 9.4 1区生物学 Q1 Agricultural and Biological Sciences

New Phytologist Pub Date : 2023-06-27 DOI:10.1111/nph.19070

Min Ning, Shi Jia Liu, Fenglin Deng, Liyu Huang, Hu Li, Jing Che, Naoki Yamaji, Fengyi Hu, Gui Jie Lei

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引用次数: 1

摘要

稻米是锌(Zn)的不良膳食来源，但却是人类镉(Cd)的主要来源;然而，它们在水稻籽粒中积累的分子机制尚不完全清楚。本研究对tono质体定位转运蛋白OsMTP1进行了功能表征。OsMTP1在种子的根、糊粉层和胚中优先表达。敲除OsMTP1降低了根细胞液、根、糊粉层和胚中的Zn浓度，随后增加了茎部和精米(胚乳)中的Zn浓度，但不影响产量。OsMTP1单倍型分析发现精米中存在与锌水平升高相关的精英等位基因，主要是由于OsMTP1转录本的减少。酵母中OsMTP1的表达增强了对Zn的耐受性，但对Cd的耐受性没有影响。而敲除OsMTP1导致植物和水稻对Cd的吸收、转运和积累减少，这可能是锌积累改变的间接影响。我们的研究结果表明，水稻OsMTP1主要作为一种定位于细胞质的转运体，将锌吸附到液泡中。OsMTP1基因敲除提高了精米锌浓度，但抑制了镉沉积，但不影响产量。因此，OsMTP1是水稻籽粒提高Zn水平和降低Cd水平的候选基因。

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A vacuolar transporter plays important roles in zinc and cadmium accumulation in rice grain

Rice grain is a poor dietary source of zinc (Zn) but the primary source of cadmium (Cd) for humans; however, the molecular mechanisms for their accumulation in rice grain remain incompletely understood.
This study functionally characterized a tonoplast-localized transporter, OsMTP1. OsMTP1 was preferentially expressed in the roots, aleurone layer, and embryo of seeds. OsMTP1 knockout decreased Zn concentration in the root cell sap, roots, aleurone layer and embryo, and subsequently increased Zn concentration in shoots and polished rice (endosperm) without yield penalty. OsMTP1 haplotype analysis revealed elite alleles associated with increased Zn level in polished rice, mostly because of the decreased OsMTP1 transcripts.
OsMTP1 expression in yeast enhanced Zn tolerance but did not affect that of Cd. While OsMTP1 knockout resulted in decreased uptake, translocation and accumulation of Cd in plant and rice grain, which could be attributed to the indirect effects of altered Zn accumulation.
Our results suggest that rice OsMTP1 primarily functions as a tonoplast-localized transporter for sequestrating Zn into vacuole. OsMTP1 knockout elevated Zn concentration but prevented Cd deposition in polished rice without yield penalty. Thus, OsMTP1 is a candidate gene for enhancing Zn level and reducing Cd level in rice grains.

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来源期刊

New Phytologist PLANT SCIENCES-

CiteScore

17.60

自引率

5.30%

发文量

728

审稿时长

1 months

期刊介绍： New Phytologist is a leading publication that showcases exceptional and groundbreaking research in plant science and its practical applications. With a focus on five distinct sections - Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology - the journal covers a wide array of topics ranging from cellular processes to the impact of global environmental changes. We encourage the use of interdisciplinary approaches, and our content is structured to reflect this. Our journal acknowledges the diverse techniques employed in plant science, including molecular and cell biology, functional genomics, modeling, and system-based approaches, across various subfields.