{"title":"lr24介导的小麦抗叶锈病需要TaRAR1","authors":"Li-rong ZHANG, Wen-xiang YANG, Da-qun LIU","doi":"10.1016/S1671-2927(11)60173-9","DOIUrl":null,"url":null,"abstract":"<div><h3>Abstract</h3><p>Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of <em>TaRAR1</em> caused conversion of incompatible to compatible interactions to <em>Lr24</em>-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring <em>Lr24</em> whereas infection with a control construct had no effect on resistance or susceptibility. RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that <em>TaRAR1</em> was a required component for <em>Lr24</em>-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.</p></div>","PeriodicalId":7475,"journal":{"name":"Agricultural Sciences in China","volume":"10 11","pages":"Pages 1732-1738"},"PeriodicalIF":0.0000,"publicationDate":"2011-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60173-9","citationCount":"4","resultStr":"{\"title\":\"TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance\",\"authors\":\"Li-rong ZHANG, Wen-xiang YANG, Da-qun LIU\",\"doi\":\"10.1016/S1671-2927(11)60173-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Abstract</h3><p>Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of <em>TaRAR1</em> caused conversion of incompatible to compatible interactions to <em>Lr24</em>-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring <em>Lr24</em> whereas infection with a control construct had no effect on resistance or susceptibility. RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that <em>TaRAR1</em> was a required component for <em>Lr24</em>-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.</p></div>\",\"PeriodicalId\":7475,\"journal\":{\"name\":\"Agricultural Sciences in China\",\"volume\":\"10 11\",\"pages\":\"Pages 1732-1738\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2011-11-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1016/S1671-2927(11)60173-9\",\"citationCount\":\"4\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Agricultural Sciences in China\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1671292711601739\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Agricultural Sciences in China","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1671292711601739","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance
Abstract
Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
