蛋拟杆菌KdgF酶的1H, 13C, 15N共振分配

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2022-08-30 DOI:10.1007/s12104-022-10102-6

Agnes Beenfeldt Petersen, Idd Andrea Christensen, Mette E. Rønne, Emil G. P. Stender, David Teze, Birte Svensson, Finn Lillelund Aachmann

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引用次数: 0

摘要

为了充分利用海藻生物量中的碳水化合物，必须了解海藻酸盐多糖家族的降解。海藻酸盐降解的一个步骤是由酶KdgF催化的4,5-不饱和单醛酸盐转化为4-脱氧- l -红-5-己糖糖。在这项研究中，来自人类肠道微生物群的蛋氏拟杆菌的BeKdgF在大肠杆菌中被同位素标记。这里报告了BeKdgF的1H, 13C和15N核磁共振化学位移分配。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

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1H, 13C, 15N resonance assignment of the enzyme KdgF from Bacteroides eggerthii

To fully utilize carbohydrates from seaweed biomass, the degradation of the family of polysaccharides known as alginates must be understood. A step in the degradation of alginate is the conversion of 4,5-unsaturated monouronates to 4-deoxy-L-erythro-5-hexoseulose catalysed by the enzyme KdgF. In this study BeKdgF from Bacteroides eggerthii from the human gut microbiota has been produced isotopically labelled in Escherichia coli. Here the ¹H, ¹³C, and ¹⁵N NMR chemical shift assignment for BeKdgF is reported.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.