聚乙烯亚胺，一种具有成本效益的转染试剂

Signal transduction Pub Date : 2006-06-01 DOI:10.1002/SITA.200500073

C. Ehrhardt, M. Schmolke, Andreas T. Matzke, Alexander Knoblauch, C. Will, V. Wixler, S. Ludwig

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引用次数: 165

摘要

为了寻找一种便宜而有效的转染试剂，我们使用了带正电荷的聚物形成化合物聚乙烯亚胺(PEI)。这种化合物在商业上可以从不同的公司获得，要么作为非改性化学试剂，要么作为成本更高的转染试剂与添加剂一起使用。在这里，我们使用未经修饰的PEI试剂来优化不同细胞系的转染方案。在这些优化的条件下，我们能够瞬时转染许多细胞系，转染率高达40-90%。

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Polyethylenimine, a cost-effective transfection reagent

In search for a cheap and effective transfection reagent we used the positively charged polyplex-forming compound polyethylenimine (PEI). This compound is commercially available from different companies either as a non-modified chemical reagent or with additives as a more cost intensive transfection reagent. Here we used the non-modified PEI reagent to optimize transfection protocols for different cell-lines. With these optimized conditions we were able to transiently transfect a number of cell-lines up to 40–90%.

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Signal transduction

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