Ahad Al-Rashdi, Abdullah Mohammed Al-Sadi, Majida Mohammed Ali Al-Harrasi, Jamal Nasser Al-Sabahi, Rhonda Janke, Rethinasamy Velazhahan
{"title":"NaCl对灰刺内生拮抗细菌生长及挥发性代谢产物的影响","authors":"Ahad Al-Rashdi, Abdullah Mohammed Al-Sadi, Majida Mohammed Ali Al-Harrasi, Jamal Nasser Al-Sabahi, Rhonda Janke, Rethinasamy Velazhahan","doi":"10.1007/s13313-023-00922-y","DOIUrl":null,"url":null,"abstract":"<div><p>Previously, we reported that endophytic <i>Pseudomonas aeruginosa</i> PC5 isolated from <i>Prosopis cineraria</i>, abundantly produced antimicrobial volatile organic compounds (VOCs) and significantly suppressed the growth of <i>Pythium aphanidermatum</i> which causes damping-off in cucumber in in vitro assays. However, under pot culture conditions, no significant effect of this bacterium on cucumber damping-off was noticed under salt water irrigation (100 mM NaCl), while the antagonistic bacterium <i>Acinetobacter johnsonii</i> PC3 isolated from the same host which produced low levels of antimicrobial VOCs was effective in reducing the disease incidence under salt water irrigation. In this in vitro study, the effect of NaCl on the growth <i>P. aphanidermatum</i> and VOC composition of these two endophytic bacterial strains was evaluated. NaCl had inhibitory effects on the growth of both <i>P. aeruginosa</i> PC5 and <i>A. johnsonii</i> PC3. Gas chromatography-mass spectrometry analysis of the VOCs produced by these endophytes revealed that dimethyl disulfide (DMDS), a well-known antimicrobial compound, was the major compound (33.82%) released by <i>P. aeruginosa</i> PC5 in the absence of NaCl, whereas the production of DMDS was completely inhibited when it was cultured in a medium containing high concentrations of NaCl. Trimethylsilanol was released abundantly by <i>A. johnsonii</i> PC3 when grown in the salt-amended medium compared to control, suggesting possible involvement of this compound in the suppression of <i>P. aphanidermatum</i> by this bacterium under salt-stress conditions.</p></div>","PeriodicalId":8598,"journal":{"name":"Australasian Plant Pathology","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2023-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13313-023-00922-y.pdf","citationCount":"1","resultStr":"{\"title\":\"The effect of NaCl on growth and volatile metabolites produced by antagonistic endophytic bacteria isolated from Prosopis cineraria\",\"authors\":\"Ahad Al-Rashdi, Abdullah Mohammed Al-Sadi, Majida Mohammed Ali Al-Harrasi, Jamal Nasser Al-Sabahi, Rhonda Janke, Rethinasamy Velazhahan\",\"doi\":\"10.1007/s13313-023-00922-y\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Previously, we reported that endophytic <i>Pseudomonas aeruginosa</i> PC5 isolated from <i>Prosopis cineraria</i>, abundantly produced antimicrobial volatile organic compounds (VOCs) and significantly suppressed the growth of <i>Pythium aphanidermatum</i> which causes damping-off in cucumber in in vitro assays. However, under pot culture conditions, no significant effect of this bacterium on cucumber damping-off was noticed under salt water irrigation (100 mM NaCl), while the antagonistic bacterium <i>Acinetobacter johnsonii</i> PC3 isolated from the same host which produced low levels of antimicrobial VOCs was effective in reducing the disease incidence under salt water irrigation. In this in vitro study, the effect of NaCl on the growth <i>P. aphanidermatum</i> and VOC composition of these two endophytic bacterial strains was evaluated. NaCl had inhibitory effects on the growth of both <i>P. aeruginosa</i> PC5 and <i>A. johnsonii</i> PC3. Gas chromatography-mass spectrometry analysis of the VOCs produced by these endophytes revealed that dimethyl disulfide (DMDS), a well-known antimicrobial compound, was the major compound (33.82%) released by <i>P. aeruginosa</i> PC5 in the absence of NaCl, whereas the production of DMDS was completely inhibited when it was cultured in a medium containing high concentrations of NaCl. Trimethylsilanol was released abundantly by <i>A. johnsonii</i> PC3 when grown in the salt-amended medium compared to control, suggesting possible involvement of this compound in the suppression of <i>P. aphanidermatum</i> by this bacterium under salt-stress conditions.</p></div>\",\"PeriodicalId\":8598,\"journal\":{\"name\":\"Australasian Plant Pathology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2023-05-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://link.springer.com/content/pdf/10.1007/s13313-023-00922-y.pdf\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Australasian Plant Pathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s13313-023-00922-y\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australasian Plant Pathology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13313-023-00922-y","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
The effect of NaCl on growth and volatile metabolites produced by antagonistic endophytic bacteria isolated from Prosopis cineraria
Previously, we reported that endophytic Pseudomonas aeruginosa PC5 isolated from Prosopis cineraria, abundantly produced antimicrobial volatile organic compounds (VOCs) and significantly suppressed the growth of Pythium aphanidermatum which causes damping-off in cucumber in in vitro assays. However, under pot culture conditions, no significant effect of this bacterium on cucumber damping-off was noticed under salt water irrigation (100 mM NaCl), while the antagonistic bacterium Acinetobacter johnsonii PC3 isolated from the same host which produced low levels of antimicrobial VOCs was effective in reducing the disease incidence under salt water irrigation. In this in vitro study, the effect of NaCl on the growth P. aphanidermatum and VOC composition of these two endophytic bacterial strains was evaluated. NaCl had inhibitory effects on the growth of both P. aeruginosa PC5 and A. johnsonii PC3. Gas chromatography-mass spectrometry analysis of the VOCs produced by these endophytes revealed that dimethyl disulfide (DMDS), a well-known antimicrobial compound, was the major compound (33.82%) released by P. aeruginosa PC5 in the absence of NaCl, whereas the production of DMDS was completely inhibited when it was cultured in a medium containing high concentrations of NaCl. Trimethylsilanol was released abundantly by A. johnsonii PC3 when grown in the salt-amended medium compared to control, suggesting possible involvement of this compound in the suppression of P. aphanidermatum by this bacterium under salt-stress conditions.
期刊介绍:
Australasian Plant Pathology presents new and significant research in all facets of the field of plant pathology. Dedicated to a worldwide readership, the journal focuses on research in the Australasian region, including Australia, New Zealand and Papua New Guinea, as well as the Indian, Pacific regions.
Australasian Plant Pathology is the official journal of the Australasian Plant Pathology Society.