透明Testa8(TT8)基因和Homeobox12(HB12)基因沉默对反刍动物系统中能量、降解和发酵特性相关分子结构谱的影响

IF 6.3
Yaogeng Lei , Abdelali Hannoufa , Peiqiang Yu
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引用次数: 1

摘要

苜蓿(Medicago sativa L.)是一种豆科牧草,因其生物量高、营养价值高而被广泛种植。然而,苜蓿含有相对较高的木质素,这限制了其利用。已经提出下调两种转录因子,透明Testa8(TT8)和Homeobox12(HB12),以降低苜蓿中的木质素含量。因此,通过RNAi技术实现了苜蓿中TT8(TT8i)和HB12(HB12i)的沉默。该项目的目的是通过沉默苜蓿植物中的TT8和HB12基因来确定基因修饰对木质素和酚含量、生物能值、瘤胃可降解和不可降解组分的营养供应以及响应苜蓿中TT8和HB12基因沉默的体外氨生产的影响。所有基因沉默的苜蓿植株(5株TT8i和11株HB12i)均在温室条件下以野生型为对照生长。分析了反刍动物系统中的生物活性化合物、降解组分、真正可消化的营养物质、能量值和体外氨生成。此外,利用振动分子光谱法确定了理化、代谢和发酵特性与分子光谱参数之间的关系。结果表明,HB12i具有较高的木质素含量,而TT8i则具有较高的酚类物质含量。两种沉默基因型都具有较高的瘤胃慢降解碳水化合物组分和真正可消化的中性洗涤纤维,但瘤胃可降解蛋白质组分较低。此外,与其他沉默基因型相比,HB12i的真正可消化粗蛋白、能量值和氨产量较低。此外,与苜蓿的营养价值相关,结构碳水化合物参数呈负相关,而蛋白质结构中的α/β比率呈正相关。此外,从分子光谱参数中获得了对蛋白质和碳水化合物组分的降解以及能量值的良好预测。总之,TT8和HB12基因的沉默降低了蛋白质的有效性,增加了纤维的有效性。HB12基因的沉默也增加了木质素,降低了能量和瘤胃氨的产生。此外,营养变化与分子光谱参数密切相关。因此,通过沉默苜蓿中的TT8和HB12基因进行基因修饰会影响苜蓿的理化、代谢和发酵特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Effects of Transparent Testa8 (TT8) gene and Homeobox12 (HB12) gene silencing in alfalfa (Medicago sativa L.) on molecular structure spectral profile in relation to energy, degradation, and fermentation characteristics in ruminant systems

Effects of Transparent Testa8 (TT8) gene and Homeobox12 (HB12) gene silencing in alfalfa (Medicago sativa L.) on molecular structure spectral profile in relation to energy, degradation, and fermentation characteristics in ruminant systems

Alfalfa (Medicago sativa L.) is a legume forage that is widely cultivated owing to its high biomass yield and favorable nutrient values. However, alfalfa contains relatively high lignin, which limits its utilization. Downregulation of two transcriptional factors, Transparent Testa8 (TT8) and Homeobox12 (HB12), has been proposed to reduce lignin content in alfalfa. Therefore, silencing of TT8 (TT8i) and HB12 (HB12i) in alfalfa was achieved by RNAi technology. The objective of this project was to determine effect of gene modification through silencing of TT8 and HB12 genes in alfalfa plants on lignin and phenolic content, bioenergic value, nutrient supply from rumen degradable and undegradable fractions, and in vitro ammonia production in response to the silencing of TT8 and HB12 genes in alfalfa. All gene silenced alfalfa plants (5 TT8i and 11 HB12i) were grown under greenhouse conditions with wild type as a control. Samples were analyzed for bioactive compounds, degradation fractions, truly digestible nutrients, energetic values and in vitro ammonia productions in ruminant systems. Furthermore, relationships between physiochemical, metabolic and fermentation characteristics and molecular spectral parameters were determined using vibrational molecular spectroscopy. Results showed that the HB12i had higher lignin, while TT8i had higher phenolics. Both silenced genotypes had higher rumen slowly degraded carbohydrate fractions and truly digestible neutral detergent fiber, but lower rumen degradable protein fractions. Moreover, the HB12i had lower truly digestible crude protein, energetic values and ammonia production compared with other silenced genotypes. In addition, in relation to the nutritive values of alfalfa, structural carbohydrate parameters were negatively correlated, whereas alpha/beta ratio in protein structure was positively correlated. Furthermore, good predictions were obtained for degradation of protein and carbohydrate fractions and energy values from molecular spectral parameters. In conclusion, silencing of the TT8 and HB12 genes decreased protein availability and increased fiber availability. Silencing of the HB12 gene also increased lignin and decreased energy and rumen ammonia production. Moreover, nutritional alterations were closely correlated with molecular spectral parameters. Therefore, gene modification through silencing the TT8 and HB12 genes in alfalfa influenced physiochemical, metabolic and fermentation characteristics.

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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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