Paweł Konieczka , Karolina Ferenc , Jens N. Jørgensen , Lea H.B. Hansen , Romuald Zabielski , Jarosław Olszewski , Zdzisław Gajewski , Magdalena Mazur-Kuśnirek , Dominika Szkopek , Natalia Szyryńska , Krzysztof Lipiński
{"title":"给妊娠期和哺乳期母猪喂以芽孢杆菌为基础的益生菌是改善断奶仔猪免疫力、肠道功能状态和益生菌生物膜形成的有效方法","authors":"Paweł Konieczka , Karolina Ferenc , Jens N. Jørgensen , Lea H.B. Hansen , Romuald Zabielski , Jarosław Olszewski , Zdzisław Gajewski , Magdalena Mazur-Kuśnirek , Dominika Szkopek , Natalia Szyryńska , Krzysztof Lipiński","doi":"10.1016/j.aninu.2023.03.003","DOIUrl":null,"url":null,"abstract":"<div><p>The effects of dietary probiotic supplementation with viable <em>Bacillus subtilis</em> and <em>Bacillus amyloliquefaciens</em> spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; <em>n</em> = 48) or a diet supplemented with viable spores (1.1 × 10<sup>9</sup> CFU/kg of feed) (probiotic; <em>n</em> = 48). At 7 d of age, sucking piglets (<em>n</em> = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (<em>P</em> = 0.077), improved the weaning weight (<em>P</em> = 0.039) and increased both the total creep feed consumption (<em>P</em> = 0.027) and litter gain (<em>P</em> = 0.011). Probiotics also improved the faecal score in the second (<em>P</em> = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (<em>P</em> = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (<em>P</em> = 0.050) and a lower IgG concentration in the ileal mucosa (<em>P</em> = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (<em>P</em> = 0.012) due to the presence of longer villi and larger Peyer's patches (<em>P</em> < 0.001). <em>B. subtilis</em> and <em>B. amyloliquefaciens</em> were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, <em>Bacillus</em>-based probiotic supplementation improves the health indices of sows and their piglets.</p></div>","PeriodicalId":62604,"journal":{"name":"Animal Nutrition","volume":"13 ","pages":"Pages 361-372"},"PeriodicalIF":6.3000,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning\",\"authors\":\"Paweł Konieczka , Karolina Ferenc , Jens N. Jørgensen , Lea H.B. Hansen , Romuald Zabielski , Jarosław Olszewski , Zdzisław Gajewski , Magdalena Mazur-Kuśnirek , Dominika Szkopek , Natalia Szyryńska , Krzysztof Lipiński\",\"doi\":\"10.1016/j.aninu.2023.03.003\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>The effects of dietary probiotic supplementation with viable <em>Bacillus subtilis</em> and <em>Bacillus amyloliquefaciens</em> spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; <em>n</em> = 48) or a diet supplemented with viable spores (1.1 × 10<sup>9</sup> CFU/kg of feed) (probiotic; <em>n</em> = 48). At 7 d of age, sucking piglets (<em>n</em> = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (<em>P</em> = 0.077), improved the weaning weight (<em>P</em> = 0.039) and increased both the total creep feed consumption (<em>P</em> = 0.027) and litter gain (<em>P</em> = 0.011). Probiotics also improved the faecal score in the second (<em>P</em> = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (<em>P</em> = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (<em>P</em> = 0.050) and a lower IgG concentration in the ileal mucosa (<em>P</em> = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (<em>P</em> = 0.012) due to the presence of longer villi and larger Peyer's patches (<em>P</em> < 0.001). <em>B. subtilis</em> and <em>B. amyloliquefaciens</em> were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, <em>Bacillus</em>-based probiotic supplementation improves the health indices of sows and their piglets.</p></div>\",\"PeriodicalId\":62604,\"journal\":{\"name\":\"Animal Nutrition\",\"volume\":\"13 \",\"pages\":\"Pages 361-372\"},\"PeriodicalIF\":6.3000,\"publicationDate\":\"2023-06-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Nutrition\",\"FirstCategoryId\":\"1091\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405654523000331\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Nutrition","FirstCategoryId":"1091","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405654523000331","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Feeding Bacillus-based probiotics to gestating and lactating sows is an efficient method for improving immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning
The effects of dietary probiotic supplementation with viable Bacillus subtilis and Bacillus amyloliquefaciens spores on sow performance, immunity, gut functional status and biofilm formation by probiotic bacteria in piglets at weaning were investigated. Ninety-six sows reared in a continuous farrowing system for one full cycle were fed gestation diets during the first 90 d of pregnancy and lactation diets until the end of lactation. The sows were fed a basal diet without probiotics (control; n = 48) or a diet supplemented with viable spores (1.1 × 109 CFU/kg of feed) (probiotic; n = 48). At 7 d of age, sucking piglets (n = 12/group) were provided prestarter creep feed until weaning at 28 d of age. The piglets in the probiotic group were supplemented with the same probiotic and dosage as their dams. Blood and colostrum collected from sows and ileal tissues collected from piglets on the day of weaning were used for analyses. Probiotics increased the weight of piglets (P = 0.077), improved the weaning weight (P = 0.039) and increased both the total creep feed consumption (P = 0.027) and litter gain (P = 0.011). Probiotics also improved the faecal score in the second (P = 0.013) week of life. The immunoglobulin G (IgG) concentrations in sow blood at farrowing and the IgM concentrations in piglet blood at weaning were higher in the probiotic group than in the control group (P = 0.046). The piglets from the probiotic-treated sows showed a higher IgM concentration in the ileal mucosa (P = 0.050) and a lower IgG concentration in the ileal mucosa (P = 0.021) compared with the piglets from control sows. The probiotic-treated piglets had a thicker ileal mucosa (P = 0.012) due to the presence of longer villi and larger Peyer's patches (P < 0.001). B. subtilis and B. amyloliquefaciens were detected in the probiotic-treated piglets but not the control piglets; these bacteria were present in the digesta and villus structures and formed structures resembling biofilms. Overall, Bacillus-based probiotic supplementation improves the health indices of sows and their piglets.
期刊介绍：
Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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