Saneha OR , Priya Krishna , Syam Das S , Krishnakumar IM , Litty Joseph
{"title":"探索一种专有的黑孜然油提取物作为双Orexin受体拮抗剂在恢复压力诱导的睡眠剥夺动物的压力睡眠平衡中的可能性","authors":"Saneha OR , Priya Krishna , Syam Das S , Krishnakumar IM , Litty Joseph","doi":"10.1016/j.phanu.2023.100357","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p><span><span>Orexins<span><span> act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing </span>melatonin, which is an inhibitor of orexin. It was reported that a proprietary </span></span>black cumin (</span><em>Nigella sativa</em>) oil (BCO-5) alleviated stress and improved sleep quality. The present study investigated the mechanism of action of BCO-5 using stress-induced and sleep-deprived model of rats.</p></div><div><h3>Methods</h3><p>Adult <em>Sprague Dawley</em><span> rats (n = 24) were randomised into 4 groups (Group I – Sham; Group II – Stress-induced group; Group III – BCO-5 treated normal animals; Group IV – Stress + BCO-5 (20 mg/kg b. wt.) for 14 days and monitored the behaviour and biochemical markers.</span></p></div><div><h3>Results</h3><p>The co-supplementation of BCO-5 significantly decreased the body weight, locomotor activity, rearing and grooming frequencies among Group IV animals significantly compared to Group II. The observed behaviour was also correlated with the significant decrease in orexin, corticosterone and c-fos expression levels, while an increase was observed in melatonin concentration.</p></div><div><h3>Conclusion</h3><p>Our results support the plausible role of BCO-5 as a DORA to manage stress and improve sleep.</p></div>","PeriodicalId":20049,"journal":{"name":"PharmaNutrition","volume":"26 ","pages":"Article 100357"},"PeriodicalIF":2.4000,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Exploring the possibility of a proprietary black cumin oil extract as a Dual Orexin Receptor Antagonist in restoring stress-sleep balance on stress-induced sleep deprived animals\",\"authors\":\"Saneha OR , Priya Krishna , Syam Das S , Krishnakumar IM , Litty Joseph\",\"doi\":\"10.1016/j.phanu.2023.100357\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background</h3><p><span><span>Orexins<span><span> act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing </span>melatonin, which is an inhibitor of orexin. It was reported that a proprietary </span></span>black cumin (</span><em>Nigella sativa</em>) oil (BCO-5) alleviated stress and improved sleep quality. The present study investigated the mechanism of action of BCO-5 using stress-induced and sleep-deprived model of rats.</p></div><div><h3>Methods</h3><p>Adult <em>Sprague Dawley</em><span> rats (n = 24) were randomised into 4 groups (Group I – Sham; Group II – Stress-induced group; Group III – BCO-5 treated normal animals; Group IV – Stress + BCO-5 (20 mg/kg b. wt.) for 14 days and monitored the behaviour and biochemical markers.</span></p></div><div><h3>Results</h3><p>The co-supplementation of BCO-5 significantly decreased the body weight, locomotor activity, rearing and grooming frequencies among Group IV animals significantly compared to Group II. The observed behaviour was also correlated with the significant decrease in orexin, corticosterone and c-fos expression levels, while an increase was observed in melatonin concentration.</p></div><div><h3>Conclusion</h3><p>Our results support the plausible role of BCO-5 as a DORA to manage stress and improve sleep.</p></div>\",\"PeriodicalId\":20049,\"journal\":{\"name\":\"PharmaNutrition\",\"volume\":\"26 \",\"pages\":\"Article 100357\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2023-09-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"PharmaNutrition\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2213434423000294\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"PharmaNutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213434423000294","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Exploring the possibility of a proprietary black cumin oil extract as a Dual Orexin Receptor Antagonist in restoring stress-sleep balance on stress-induced sleep deprived animals
Background
Orexins act as a molecular switch for the release of cortisol/corticosterone in response to a stress stimulus and hence to regulate sleep/wake cycle. Orexin agonism during the day promotes wakefulness, and Dual Orexin Receptor Antagonists (DORA) can promote sleep signals by enhancing melatonin, which is an inhibitor of orexin. It was reported that a proprietary black cumin (Nigella sativa) oil (BCO-5) alleviated stress and improved sleep quality. The present study investigated the mechanism of action of BCO-5 using stress-induced and sleep-deprived model of rats.
Methods
Adult Sprague Dawley rats (n = 24) were randomised into 4 groups (Group I – Sham; Group II – Stress-induced group; Group III – BCO-5 treated normal animals; Group IV – Stress + BCO-5 (20 mg/kg b. wt.) for 14 days and monitored the behaviour and biochemical markers.
Results
The co-supplementation of BCO-5 significantly decreased the body weight, locomotor activity, rearing and grooming frequencies among Group IV animals significantly compared to Group II. The observed behaviour was also correlated with the significant decrease in orexin, corticosterone and c-fos expression levels, while an increase was observed in melatonin concentration.
Conclusion
Our results support the plausible role of BCO-5 as a DORA to manage stress and improve sleep.
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