球形枝孢提取物抑制粘质沙雷氏菌群体感应相关毒力因子

IF 5.9 Q1 MICROBIOLOGY
Dan-Rui Liu , Qing-Xiang Yan , Zheng-Biao Zou , Chun-Lan Xie , Xian-Wen Yang , Ai-Qun Jia
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引用次数: 0

摘要

粘质沙雷氏菌现在正成为其高度抗微生物耐药性临床感染的倾向。目前,它通过调节粘质链霉菌群体感应(QS)提供了一种预防和控制微生物感染的新策略。深海真菌富含QS生物活性成分。在本工作中,球形球孢枝孢SCSGAF0054的提取物对粘质链霉菌NJ01表现出强大的QS相关毒力因子和生物膜抑制活性。不同浓度的提取物对游泳运动能力和多种毒力因子如灵菌肽、胞外多糖(EPS)、脂肪酶、蛋白酶和溶血素均有一定的抑制作用。共聚焦激光扫描显微镜(CLSM)和扫描电子显微镜(SEM)图像显示,球藻提取物适度抑制了生物膜的形成和细胞活力。此外,实时定量PCR(RT-qPCR)分析显示,与对照相比,与毒力因子flhD、fimA、fimC、bsmA、bsmB、pigA、pigC和shlA相关的基因的表达显著下调。此外,与亚胺培南联合的提取物抑制了粘质链霉菌NJ01的QS系统,破坏了其预先形成的生物膜,释放了生物膜内的细菌,并逐渐杀死了细菌。因此,与亚胺培南联合使用的提取物可以部分恢复细菌的药物敏感性。这些结果表明,SCSGAF0054的提取物可以有效地干扰QS系统,单独或与经典抗菌药物联合治疗粘质链霉菌感染。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens

Serratia marcescens is now becoming a propensity for its highly antimicrobial-resistant clinical infections. Currently, it provides a novel strategy to prevent and control microbial infection by regulating S. marcescens quorum sensing (QS). Deep-sea-derived fungi are rich in QS bioactive constituents. In this work, the extracts from Cladosporium sphaerospermum SCSGAF0054 showed potent QS-related virulence factors and biofilm-inhibiting activities against S. marcescens NJ01. The swimming motility and multiple virulence factors such as prodigiosin, exopolysaccharide (EPS), lipase, protease and hemolysin were moderately inhibited by the extracts at varied concentrations. The confocal laser scanning microscope (CLSM) and scanning electron microscope (SEM) images revealed that C. sphaerospermum extracts moderately arrested biofilm formation and cell viability. Further, real-time quantitative PCR (RT-qPCR) analysis revealed that expressions of genes associated with virulence factors, flhD, fimA, fimC, bsmA, bsmB, pigA, pigC, and shlA, were significantly down-regulated compared with control. In addition, the extracts combined with imipenem inhibited the QS system of S. marcescens NJ01, disrupted its preformed biofilm, released the intra-biofilm bacteria and killed the bacteria gradually. Therefore, the extracts combined with imipenem can partially restore bacterial drug sensitivity. These results suggest that the extracts from SCSGAF0054 effectively interfere with the QS system to treat S. marcescens infection alone or combining with classical antimicrobial drugs.

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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
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