V.A.C.®发泡胶银的离体比较™ 和V.A.C.®Granufoam™ 负载一流的双二烷基去精脒三苯基抗生物膜剂

IF 5.9 Q1 MICROBIOLOGY
Kaden B. Rawson , Travis Neuberger , Tyler B. Smith , Isaac J. Bell , Ryan E. Looper , Paul R. Sebahar , Travis J. Haussener , Hariprasada Reddy Kanna Reddy , Brad M. Isaacson , John Shero , Paul F. Pasquina , Dustin L. Williams
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引用次数: 0

摘要

事实证明,负压伤口治疗(NPWT)作为一种标准的护理方法,在减少压疮和创伤、战斗相关损伤的愈合时间和医院感染的可能性方面是有效的。然而,目前的制剂可能无法靶向或显著降低治疗后的细菌生物膜负荷。本研究的目的是确定开孔聚氨酯(PU)泡沫(V.A.C.®Granufoam™) 负载第一类化合物(CZ-01179)作为活性释放剂,通过冻干水凝胶支架整合。设计了一种离体猪切除伤口模型,在存在NPWT的情况下进行抗生物膜功效测试。制备了装有10.0%w/w CZ-01179配方和0.5%透明质酸的PU泡沫样品,并根据当前的护理标准进行了测试:V.a.C.®Granufoam Silver™ 和V.A.C.®Granufoam™. 与当前标准的护理泡沫相比,我们观察到使用CZ-01179抗菌膜泡沫的耐甲氧西林金黄色葡萄球菌(MRSA)和鲍曼不动杆菌生物膜在统计学上显著减少。这些发现激发了负载CZ-01179的抗生物膜PU泡沫的进一步开发。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Ex vivo comparison of V.A.C.® Granufoam Silver™ and V.A.C.® Granufoam™ loaded with a first-in-class bis-dialkylnorspermidine-terphenyl antibiofilm agent

Implementation of negative pressure wound therapy (NPWT) as a standard of care has proven efficacious in reducing both the healing time and likelihood of nosocomial infection among pressure ulcers and traumatic, combat-related injuries. However, current formulations may not target or dramatically reduce bacterial biofilm burden following therapy. The purpose of this study was to determine the antibiofilm efficacy of an open-cell polyurethane (PU) foam (V.A.C.® Granufoam™) loaded with a first-in-class compound (CZ-01179) as the active release agent integrated via lyophilized hydrogel scaffolding. An ex vivo porcine excision wound model was designed to perform antibiofilm efficacy testing in the presence of NPWT. PU foam samples loaded with a 10.0% w/w formulation of CZ-01179 and 0.5% hyaluronic acid were prepared and tested against current standards of care: V.A.C.® Granufoam Silver™ and V.A.C.® Granufoam™. We observed statistically significant reduction of methicillin-resistant Staphylococcus aureus (MRSA) and Acinetobacter baumannii biofilms with the CZ-01179 antibiofilm foam in comparison to current standard of care foams. These findings motivate further development of an antibiofilm PU foam loaded with CZ-01179.

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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
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