性类固醇激素对体外口腔生物膜生态的影响

IF 5.9 Q1 MICROBIOLOGY
Pilar Cornejo Ulloa, Monique H. van der Veen, Bernd W. Brandt, Mark J. Buijs, Bastiaan P. Krom
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引用次数: 1

摘要

性类固醇激素(SSH),如雌激素、黄体酮和睾酮,是胆固醇衍生的分子,调节各种生理过程。它们存在于血液和唾液中,与口腔组织和口腔微生物接触。几项研究已经使用单物种模型证实了这些激素对不同牙周病相关细菌的影响。细菌可以代谢SSH,将其用作维生素K的替代品,还可以利用它们诱导毒力因子的表达。然而,目前尚不清楚SSH对口腔微生物组的影响。在本研究中,我们研究了四种SSH对体外口腔共生生物膜的影响。唾液衍生的口腔生物膜在没有血清或甲萘醌的Mc-Bain培养基中使用阿姆斯特丹活性附着模型生长。在没有SSH的情况下初始附着后,生物膜在含有100倍生理浓度的雌二醇、雌二醇、孕酮或睾酮的培养基中生长。甲萘醌或乙醇分别作为阳性对照和阴性对照。每天更新培养基12天后,测定生物膜的形成、生物膜红色荧光和微生物组成。使用荧光共振能量转移分析(FRET)测试上清液的蛋白水解活性。在任何测试组中,在生物膜形成、红色荧光或微生物组成方面都没有发现显著差异。在黄体酮和雌二醇存在下生长的样品显示出与补充甲萘醌的生物膜相当的蛋白水解活性。相反,与在甲萘醌存在下生长的生物膜相比,睾酮和雌二醇的蛋白水解活性降低。没有一种测试的SSH对体外口腔生物膜的生态有很大影响,因此不可能将我们的结果直接转化为体内影响。未来的实验应该包括其他宿主因素,如口腔组织、免疫细胞和唾液中存在的SSH组合,以便更准确地了解男性和女性中发生的现象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The effect of sex steroid hormones on the ecology of in vitro oral biofilms

The effect of sex steroid hormones on the ecology of in vitro oral biofilms

Sex steroid hormones (SSH) such as oestrogen, progesterone and testosterone are cholesterol derived molecules that regulate various physiological processes. They are present in both blood and saliva, where they come in contact with oral tissues and oral microorganisms. Several studies have confirmed the effect of these hormones on different periodontal-disease-associated bacteria, using single-species models. Bacteria can metabolize SSH, use them as alternative for vitamin K and also use them to induce the expression of virulence factors. However, it is still unclear what the effects of SSH are on the oral microbiome. In this study, we investigated the effects of four SSH on commensal in vitro oral biofilms. Saliva-derived oral biofilms were grown in Mc Bain medium without serum or menadione using the Amsterdam Active-Attachment model. After initial attachment in absence of SSH, the biofilms were grown in medium containing either oestradiol, oestriol, progesterone or testosterone at a 100-fold physiological concentration. Menadione or ethanol were included as positive control and negative control, respectively. After 12 days with daily medium refreshments, biofilm formation, biofilm red fluorescence and microbial composition were determined. The supernatants were tested for proteolytic activity using the Fluorescence Resonance Energy Transfer Analysis (FRET). No significant differences were found in biofilm formation, red fluorescence or microbial composition in any of the tested groups. Samples grown in presence of progesterone and oestradiol showed proteolytic activity comparable to biofilms supplemented with menadione. In contrast, testosterone and oestriol showed a decreased proteolytic activity compared to biofilms grown in presence of menadione.

None of the tested SSH had large effects on the ecology of in vitro oral biofilms, therefore a direct translation of our results into in vivo effects is not possible. Future experiments should include other host factors such as oral tissues, immune cells and combinations of SSH as present in saliva, in order to have a more accurate picture of the phenomena taking place in both males and females.

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来源期刊
Biofilm
Biofilm MICROBIOLOGY-
CiteScore
7.50
自引率
1.50%
发文量
30
审稿时长
57 days
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