研究抑制肽对单核细胞增多性李斯特菌细胞侵袭的影响：一项计算机和体外研究。

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2023-10-25 DOI:10.1186/s13099-023-00576-7

Ali Shivaee, Sara Bahonar, Mehdi Goudarzi, Ali Hematian, Bahareh Hajikhani, Behrooz Sadeghi Kalani

{"title":"研究抑制肽对单核细胞增多性李斯特菌细胞侵袭的影响：一项计算机和体外研究。","authors":"Ali Shivaee, Sara Bahonar, Mehdi Goudarzi, Ali Hematian, Bahareh Hajikhani, Behrooz Sadeghi Kalani","doi":"10.1186/s13099-023-00576-7","DOIUrl":null,"url":null,"abstract":"Aims: L.monocytogenes monocytogenes is an omnipresent bacterium that causes a fatal food-borne illness, listeriosis. The connection of this bacterium to E-cadherin through internalin A plays a significant role in the internalization of the bacteria. In this study, this interaction has been investigated for the design of an inhibitory peptide.Methods: The interaction of the proteins involved in the entry of bacteria was evaluated by molecular docking. According to their interactions, an inhibitory peptide was designed to bind to internalin A by server peptiderive. Its effects on L.monocytogenes invasion on the Caco-2 cell line and biofilm formation were also assessed.Findings: Docking results showed that the peptide has a high affinity for binding to Internalin A. The synthesized peptide at a concentration of 64 µg/ml inhibited 80% of the invasion of L.monocytogenes into the Caco-2 cell line. Furthermore, the studied peptide at the highest concentration had a slight inhibitory effect on biofilm formation.Conclusion: These results reveal that short polypeptides can impede the invasion of target cells by L. monocytogenes in vitro and could be advantageous as restoring agents in vivo.","PeriodicalId":12833,"journal":{"name":"Gut Pathogens","volume":"15 1","pages":"51"},"PeriodicalIF":4.3000,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601259/pdf/","citationCount":"0","resultStr":"{\"title\":\"Investigating the effect of the inhibitory peptide on L.monocytogenes cell invasion: an in silico and in vitro study.\",\"authors\":\"Ali Shivaee, Sara Bahonar, Mehdi Goudarzi, Ali Hematian, Bahareh Hajikhani, Behrooz Sadeghi Kalani\",\"doi\":\"10.1186/s13099-023-00576-7\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aims: L.monocytogenes monocytogenes is an omnipresent bacterium that causes a fatal food-borne illness, listeriosis. The connection of this bacterium to E-cadherin through internalin A plays a significant role in the internalization of the bacteria. In this study, this interaction has been investigated for the design of an inhibitory peptide.Methods: The interaction of the proteins involved in the entry of bacteria was evaluated by molecular docking. According to their interactions, an inhibitory peptide was designed to bind to internalin A by server peptiderive. Its effects on L.monocytogenes invasion on the Caco-2 cell line and biofilm formation were also assessed.Findings: Docking results showed that the peptide has a high affinity for binding to Internalin A. The synthesized peptide at a concentration of 64 µg/ml inhibited 80% of the invasion of L.monocytogenes into the Caco-2 cell line. Furthermore, the studied peptide at the highest concentration had a slight inhibitory effect on biofilm formation.Conclusion: These results reveal that short polypeptides can impede the invasion of target cells by L. monocytogenes in vitro and could be advantageous as restoring agents in vivo.\",\"PeriodicalId\":12833,\"journal\":{\"name\":\"Gut Pathogens\",\"volume\":\"15 1\",\"pages\":\"51\"},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2023-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10601259/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Gut Pathogens\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1186/s13099-023-00576-7\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"GASTROENTEROLOGY & HEPATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Gut Pathogens","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1186/s13099-023-00576-7","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"GASTROENTEROLOGY & HEPATOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

目的：单核细胞增多性李斯特菌单核细胞增生性李斯特菌是一种普遍存在的细菌，可引起致命的食源性疾病李斯特菌病。这种细菌通过内化蛋白A与E-钙粘蛋白的连接在细菌的内化中起着重要作用。在这项研究中，这种相互作用已经被研究用于抑制肽的设计。方法：利用分子对接技术对细菌进入过程中所涉及的蛋白质间的相互作用进行评价。根据它们的相互作用，设计了一种抑制肽，通过服务肽肽与内化蛋白A结合。还评估了其对单核细胞增多性李斯特菌对Caco-2细胞系的侵袭和生物膜形成的影响。研究结果：对接结果表明，该肽对与Internalin a的结合具有高亲和力。合成的肽在64µg/ml的浓度下抑制了80%的单核细胞增多性李斯特菌对Caco-2细胞系的侵袭。此外，所研究的肽在最高浓度下对生物膜的形成具有轻微的抑制作用。结论：这些结果表明，短多肽在体外可以阻止单核细胞增多性李斯特菌对靶细胞的侵袭，并有利于作为体内修复剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Investigating the effect of the inhibitory peptide on L.monocytogenes cell invasion: an in silico and in vitro study.

查看原文本刊更多论文

Investigating the effect of the inhibitory peptide on L.monocytogenes cell invasion: an in silico and in vitro study.

Aims: L.monocytogenes monocytogenes is an omnipresent bacterium that causes a fatal food-borne illness, listeriosis. The connection of this bacterium to E-cadherin through internalin A plays a significant role in the internalization of the bacteria. In this study, this interaction has been investigated for the design of an inhibitory peptide.

Methods: The interaction of the proteins involved in the entry of bacteria was evaluated by molecular docking. According to their interactions, an inhibitory peptide was designed to bind to internalin A by server peptiderive. Its effects on L.monocytogenes invasion on the Caco-2 cell line and biofilm formation were also assessed.

Findings: Docking results showed that the peptide has a high affinity for binding to Internalin A. The synthesized peptide at a concentration of 64 µg/ml inhibited 80% of the invasion of L.monocytogenes into the Caco-2 cell line. Furthermore, the studied peptide at the highest concentration had a slight inhibitory effect on biofilm formation.

Conclusion: These results reveal that short polypeptides can impede the invasion of target cells by L. monocytogenes in vitro and could be advantageous as restoring agents in vivo.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).