Erinn P Mills, Chin-Chi Liu, Melanie A Mironovich, Christopher M Taylor, Meng Luo, Ugochi Emelogu, Erin M Scott, Marina L Leis, Renee T Carter, Pilar Camacho-Luna, Andrew C Lewin
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Bacterial DNA was extracted and submitted for 16S rRNA gene sequencing. Real-time polymerase chain reaction was performed for selected bacterial species. Overall DNA concentration between groups was assessed.</p><p><strong>Results: </strong>Bacterial microbiota relative abundance composition was significantly different between ImpOut and WorOut groups (weighted UniFrac p = .006). Alpha diversity was significantly higher in the ImpOut group compared with the WorOut group (Shannon p = .042, Simpson's p = .022, Pielou's p = .037). Differences in the relative abundance of various phyla and species were detected between groups. Total DNA concentration was higher in the WorOut group compared with the ImpOut group (p = .04). Feline GAPDH (p = .001) and Bilophila wadsworthia (p = .024) copy number was significantly higher in the ImpOut group compared with the WorOut group.</p><p><strong>Conclusions: </strong>The results highlight the important relationship between the bacterial ocular surface microbiota and FHV-1 infection outcomes in cats treated with antiviral medications. Low bacterial species diversity, higher overall DNA (presumed predominantly bacterial) load, and certain bacterial phyla/species were associated with poor outcomes for cats with FHV-1 ocular disease.</p>","PeriodicalId":23836,"journal":{"name":"Veterinary ophthalmology","volume":" ","pages":"318-329"},"PeriodicalIF":1.7000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Relationship between the bacterial ocular surface microbiota and outcomes for cats with feline herpesvirus type 1 ocular surface disease.\",\"authors\":\"Erinn P Mills, Chin-Chi Liu, Melanie A Mironovich, Christopher M Taylor, Meng Luo, Ugochi Emelogu, Erin M Scott, Marina L Leis, Renee T Carter, Pilar Camacho-Luna, Andrew C Lewin\",\"doi\":\"10.1111/vop.13157\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>Feline herpesvirus 1 (FHV-1) causes ocular surface disease in domestic cats. 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引用次数: 0
摘要
目的：猫疱疹病毒1型（FHV-1）引起家猫眼表疾病。本研究的目的是评估细菌眼表微生物群与FHV-1眼表疾病猫预后之间的关系。研究动物：22只收容所饲养的猫确诊患有FHV-1眼表疾病。程序：根据干预后FHV-1脱落和眼部临床评分对动物进行分组：结果恶化（WorOut，n = 11） 或改善的结果（ImpOut，n = 11） 。在每天两次抗病毒治疗的第1天和第8天完成评分和结膜取样。提取细菌DNA并提交16S rRNA基因测序。对选定的细菌种类进行实时聚合酶链式反应。评估各组之间的总体DNA浓度。结果：ImpOut组和WorOut组的细菌微生物群相对丰度组成显著不同（加权UniFrac p = .006）。与WorOut组相比，ImpOut组的Alpha多样性显著更高（Shannon p = .042，Simpson's p = .022，Pielou's p = .037）。各组之间发现不同门和物种的相对丰度存在差异。WorOut组的总DNA浓度高于ImpOut组（p = .04）。猫科动物GAPDH（p = .001）和华氏毕氏菌（p = .024）拷贝数在ImpOut组中显著高于WorOut组。结论：研究结果强调了接受抗病毒药物治疗的猫的细菌眼表微生物群与FHV-1感染结果之间的重要关系。低细菌物种多样性、较高的总DNA（推测主要是细菌）负荷和某些细菌门/种与FHV-1眼病猫的不良结果有关。
Relationship between the bacterial ocular surface microbiota and outcomes for cats with feline herpesvirus type 1 ocular surface disease.
Objective: Feline herpesvirus 1 (FHV-1) causes ocular surface disease in domestic cats. The purpose of this study was to assess the relationship between bacterial ocular surface microbiota and outcomes for cats with FHV-1 ocular surface disease.
Procedures: Animals were grouped according to FHV-1 shedding and ocular clinical scores following intervention: worsened outcome (WorOut, n = 11) or improved outcome (ImpOut, n = 11). Scoring and conjunctival sampling were completed on Days 1 and 8 of twice daily antiviral treatment. Bacterial DNA was extracted and submitted for 16S rRNA gene sequencing. Real-time polymerase chain reaction was performed for selected bacterial species. Overall DNA concentration between groups was assessed.
Results: Bacterial microbiota relative abundance composition was significantly different between ImpOut and WorOut groups (weighted UniFrac p = .006). Alpha diversity was significantly higher in the ImpOut group compared with the WorOut group (Shannon p = .042, Simpson's p = .022, Pielou's p = .037). Differences in the relative abundance of various phyla and species were detected between groups. Total DNA concentration was higher in the WorOut group compared with the ImpOut group (p = .04). Feline GAPDH (p = .001) and Bilophila wadsworthia (p = .024) copy number was significantly higher in the ImpOut group compared with the WorOut group.
Conclusions: The results highlight the important relationship between the bacterial ocular surface microbiota and FHV-1 infection outcomes in cats treated with antiviral medications. Low bacterial species diversity, higher overall DNA (presumed predominantly bacterial) load, and certain bacterial phyla/species were associated with poor outcomes for cats with FHV-1 ocular disease.
期刊介绍：
Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of:
Clinical and investigational veterinary and comparative ophthalmology;
Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species;
Experimental models of both animal and human ocular disease in veterinary species;
Anatomic studies of the animal eye;
Physiological studies of the animal eye;
Pharmacological studies of the animal eye.
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