一个经典的筛选标记不影响触角电生理,但强烈调节背小实蝇的生殖行为。

IF 2.3 2区 农林科学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
QiQige Wuyun, Yan Zhang, Jinxi Yuan, Jie Zhang, Cong Ren, Qi Wang, Shanchun Yan, Wei Liu, Guirong Wang
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引用次数: 0

摘要

几十年来,在不育昆虫技术项目中,关键表型白眼(白色)一直被用于在释放前选择性地去除雌性,并作为基因工程中的一种有效筛选标记。桔小实蝇是一种具有代表性的热带害虫,对150多种果树造成危害。然而,白色在重要的生物学过程中的作用在B.dorsalis中仍不清楚。在本研究中,测试了白色基因对B.dorsalis的电生理和生殖行为的影响。结果表明,敲除Bdwhite会破坏成年人眼睛的色素沉着,这与之前的报道一致。Bdwhite对63种不同结构的化学成分的触角电生理反应没有影响。然而,在Bdwhite-/-中,雄性和雌性的生殖行为都显著减少。Bdwhite-/-的交配前和交配行为都显著减少,而且这种影响是雄性特有的。突变雌性对γ-八内酯的产卵显著延迟,对橙汁的产卵行为峰值消失。这些结果表明,在功能基因研究中,Bdwhite可能不是一个理想的筛选标记,目的是识别行为改变化学物质的分子靶标。相反,由于其对B.dorsalis性行为的强烈影响,由Bdwhite调节的下游基因或来自白色连接区域的基因可能是促进开发更好的基于化学方法的行为改变害虫管理技术的替代分子靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A classic screening marker does not affect antennal electrophysiology but strongly regulates reproductive behaviours in Bactrocera dorsalis

A classic screening marker does not affect antennal electrophysiology but strongly regulates reproductive behaviours in Bactrocera dorsalis

A classic screening marker does not affect antennal electrophysiology but strongly regulates reproductive behaviours in Bactrocera dorsalis

The key phenotype white eye (white) has been used for decades to selectively remove females before release in sterile insect technique programs and as an effective screening marker in genetic engineering. Bactrocera dorsalis is a representative tephritid pest causing damage to more than 150 fruit crops. Yet, the function of white in important biological processes remains unclear in B. dorsalis. In this study, the impacts of the white gene on electrophysiology and reproductive behaviour in B. dorsalis were tested. The results indicated that knocking out Bdwhite disrupted eye pigmentation in adults, consistent with previous reports. Bdwhite did not affect the antennal electrophysiology response to 63 chemical components with various structures. However, reproductive behaviours in both males and females were significantly reduced in Bdwhite−/−. Both pre-copulatory and copulation behaviours were significantly reduced in Bdwhite−/−, and the effect was male-specific. Mutant females significantly delayed their oviposition towards γ-octalactone, and the peak of oviposition behaviour towards orange juice was lost. These results show that Bdwhite might not be an ideal screening marker in functional gene research aiming to identify molecular targets for behaviour-modifying chemicals. Instead, owing to its strong effect on B. dorsalis sexual behaviours, the downstream genes regulated by Bdwhite or the genes from white-linked areas could be alternate molecular targets that promote the development of better behavioural modifying chemical-based pest management techniques.

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来源期刊
Insect Molecular Biology
Insect Molecular Biology 生物-昆虫学
CiteScore
4.80
自引率
3.80%
发文量
68
审稿时长
6-12 weeks
期刊介绍: Insect Molecular Biology has been dedicated to providing researchers with the opportunity to publish high quality original research on topics broadly related to insect molecular biology since 1992. IMB is particularly interested in publishing research in insect genomics/genes and proteomics/proteins. This includes research related to: • insect gene structure • control of gene expression • localisation and function/activity of proteins • interactions of proteins and ligands/substrates • effect of mutations on gene/protein function • evolution of insect genes/genomes, especially where principles relevant to insects in general are established • molecular population genetics where data are used to identify genes (or regions of genomes) involved in specific adaptations • gene mapping using molecular tools • molecular interactions of insects with microorganisms including Wolbachia, symbionts and viruses or other pathogens transmitted by insects Papers can include large data sets e.g.from micro-array or proteomic experiments or analyses of genome sequences done in silico (subject to the data being placed in the context of hypothesis testing).
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