{"title":"通过自动化原位培养系统量化远洋初级生产和呼吸","authors":"Solomon T. Chen, Collin P. Ward, Matthew H. Long","doi":"10.1002/lom3.10560","DOIUrl":null,"url":null,"abstract":"<p>Pelagic photosynthesis and respiration serve critical roles in controlling the dissolved oxygen (DO) concentration in seawater. The consumption and production via pelagic primary production are of particular importance in the surface ocean and in freshwater ecosystems where photosynthetically active radiation is abundant. However, the dynamic nature and large degree of heterogeneity in these ecosystems pose substantial challenges for providing accurate estimates of marine primary production and metabolic state. The resulting lack of higher-resolution data in these systems hinders efforts in scaling and including primary production in predictive models. To bridge the gap, we developed and validated a novel automated water incubator that measures in situ rates of photosynthesis and respiration. The automated water incubation system uses commercially available optodes and microcontrollers to record continuous measurements of DO within a closed chamber at desired intervals. With fast response optodes, the incubation system produced measurements of photosynthesis and respiration with an hourly resolution, resolving diel signals in the water column. The high temporal resolution of the time series also enabled the development of Monte Carlo simulation as a new data analysis technique to calculate DO fluxes, with improved performance in noisy time series. Deployment of the incubator was conducted near Ucantena Island, Massachusetts, U.S.A. The data captured diel fluctuations in metabolic fluxes with an hourly resolution, allowed for a more accurate correlation between oxygen cycling and environmental conditions, and provided improved characterization of the pelagic metabolic state.</p>","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"21 8","pages":"495-507"},"PeriodicalIF":2.1000,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10560","citationCount":"1","resultStr":"{\"title\":\"Quantifying pelagic primary production and respiration via an automated in situ incubation system\",\"authors\":\"Solomon T. Chen, Collin P. Ward, Matthew H. Long\",\"doi\":\"10.1002/lom3.10560\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Pelagic photosynthesis and respiration serve critical roles in controlling the dissolved oxygen (DO) concentration in seawater. The consumption and production via pelagic primary production are of particular importance in the surface ocean and in freshwater ecosystems where photosynthetically active radiation is abundant. However, the dynamic nature and large degree of heterogeneity in these ecosystems pose substantial challenges for providing accurate estimates of marine primary production and metabolic state. The resulting lack of higher-resolution data in these systems hinders efforts in scaling and including primary production in predictive models. To bridge the gap, we developed and validated a novel automated water incubator that measures in situ rates of photosynthesis and respiration. The automated water incubation system uses commercially available optodes and microcontrollers to record continuous measurements of DO within a closed chamber at desired intervals. With fast response optodes, the incubation system produced measurements of photosynthesis and respiration with an hourly resolution, resolving diel signals in the water column. The high temporal resolution of the time series also enabled the development of Monte Carlo simulation as a new data analysis technique to calculate DO fluxes, with improved performance in noisy time series. Deployment of the incubator was conducted near Ucantena Island, Massachusetts, U.S.A. The data captured diel fluctuations in metabolic fluxes with an hourly resolution, allowed for a more accurate correlation between oxygen cycling and environmental conditions, and provided improved characterization of the pelagic metabolic state.</p>\",\"PeriodicalId\":18145,\"journal\":{\"name\":\"Limnology and Oceanography: Methods\",\"volume\":\"21 8\",\"pages\":\"495-507\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-06-16\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10560\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Limnology and Oceanography: Methods\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10560\",\"RegionNum\":3,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"LIMNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography: Methods","FirstCategoryId":"89","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10560","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"LIMNOLOGY","Score":null,"Total":0}
Quantifying pelagic primary production and respiration via an automated in situ incubation system
Pelagic photosynthesis and respiration serve critical roles in controlling the dissolved oxygen (DO) concentration in seawater. The consumption and production via pelagic primary production are of particular importance in the surface ocean and in freshwater ecosystems where photosynthetically active radiation is abundant. However, the dynamic nature and large degree of heterogeneity in these ecosystems pose substantial challenges for providing accurate estimates of marine primary production and metabolic state. The resulting lack of higher-resolution data in these systems hinders efforts in scaling and including primary production in predictive models. To bridge the gap, we developed and validated a novel automated water incubator that measures in situ rates of photosynthesis and respiration. The automated water incubation system uses commercially available optodes and microcontrollers to record continuous measurements of DO within a closed chamber at desired intervals. With fast response optodes, the incubation system produced measurements of photosynthesis and respiration with an hourly resolution, resolving diel signals in the water column. The high temporal resolution of the time series also enabled the development of Monte Carlo simulation as a new data analysis technique to calculate DO fluxes, with improved performance in noisy time series. Deployment of the incubator was conducted near Ucantena Island, Massachusetts, U.S.A. The data captured diel fluctuations in metabolic fluxes with an hourly resolution, allowed for a more accurate correlation between oxygen cycling and environmental conditions, and provided improved characterization of the pelagic metabolic state.
期刊介绍:
Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication.
Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.