{"title":"再生障碍性贫血患者IFN-γ+和TNF-α+粘膜相关不变T细胞增加","authors":"Xiaohui Chen, Yuping Zhang, Yikai Zhang, Yue Zhang, Shunqing Wang, Zhi Yu, Xiaoen Liu, Guixuan Huang, Lixing Guo, Xueqin Li, Xianfeng Zha, Yangqiu Li, Bo Li","doi":"10.1002/cyto.b.22115","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal-associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>We found that the percentage of circulating MAIT cells was generally higher for CD3<sup>+</sup>, CD8<sup>+</sup>, and CD8<sup>−</sup> T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL-18Rα-, NKG2D-, IFN-γ-, and TNF-α- positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN-γ<sup>+</sup> CD3<sup>+</sup> or TNF-α<sup>+</sup>CD8<sup>−</sup> MAIT cells had a significant negative correlation with the absolute neutrophil count.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN-γ and TNF-α production and may contribute to the pathogenesis of AA.</p>\n </section>\n </div>","PeriodicalId":10883,"journal":{"name":"Cytometry Part B: Clinical Cytometry","volume":"104 3","pages":"253-262"},"PeriodicalIF":2.3000,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.b.22115","citationCount":"2","resultStr":"{\"title\":\"Increased IFN-γ+ and TNF-α+ mucosal-associated invariant T cells in patients with aplastic anemia\",\"authors\":\"Xiaohui Chen, Yuping Zhang, Yikai Zhang, Yue Zhang, Shunqing Wang, Zhi Yu, Xiaoen Liu, Guixuan Huang, Lixing Guo, Xueqin Li, Xianfeng Zha, Yangqiu Li, Bo Li\",\"doi\":\"10.1002/cyto.b.22115\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal-associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>We found that the percentage of circulating MAIT cells was generally higher for CD3<sup>+</sup>, CD8<sup>+</sup>, and CD8<sup>−</sup> T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL-18Rα-, NKG2D-, IFN-γ-, and TNF-α- positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN-γ<sup>+</sup> CD3<sup>+</sup> or TNF-α<sup>+</sup>CD8<sup>−</sup> MAIT cells had a significant negative correlation with the absolute neutrophil count.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN-γ and TNF-α production and may contribute to the pathogenesis of AA.</p>\\n </section>\\n </div>\",\"PeriodicalId\":10883,\"journal\":{\"name\":\"Cytometry Part B: Clinical Cytometry\",\"volume\":\"104 3\",\"pages\":\"253-262\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-02-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/cyto.b.22115\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cytometry Part B: Clinical Cytometry\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22115\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICAL LABORATORY TECHNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cytometry Part B: Clinical Cytometry","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cyto.b.22115","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICAL LABORATORY TECHNOLOGY","Score":null,"Total":0}
Increased IFN-γ+ and TNF-α+ mucosal-associated invariant T cells in patients with aplastic anemia
Background
Aplastic anemia (AA) is known as an autoimmune disease in which T cell activation is aberrant. It has been reported that unconventional T cells, mucosal-associated invariant T (MAIT) cells, play an important role in several autoimmune diseases, but it is unclear if they are involved in AA.
Methods
In this study, we for the first time analyzed the proportions, phenotypes, and cytokine properties of MAIT cells in AA by flow cytometry.
Results
We found that the percentage of circulating MAIT cells was generally higher for CD3+, CD8+, and CD8− T cells in AA patients compared with healthy individuals. Moreover, the percentage of IL-18Rα-, NKG2D-, IFN-γ-, and TNF-α- positive MAIT cells was also significantly higher in AA patients. In addition, the percentage of IFN-γ+ CD3+ or TNF-α+CD8− MAIT cells had a significant negative correlation with the absolute neutrophil count.
Conclusions
We present the first observation of MAIT cells in patients with AA. MAIT cells are associated with a higher frequency of IFN-γ and TNF-α production and may contribute to the pathogenesis of AA.
期刊介绍：
Cytometry Part B: Clinical Cytometry features original research reports, in-depth reviews and special issues that directly relate to and palpably impact clinical flow, mass and image-based cytometry. These may include clinical and translational investigations important in the diagnostic, prognostic and therapeutic management of patients. Thus, we welcome research papers from various disciplines related [but not limited to] hematopathologists, hematologists, immunologists and cell biologists with clinically relevant and innovative studies investigating individual-cell analytics and/or separations. In addition to the types of papers indicated above, we also welcome Letters to the Editor, describing case reports or important medical or technical topics relevant to our readership without the length and depth of a full original report.
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