显微镜显示谷胱甘肽S-转移酶储存在骨髓中的大颗粒髓细胞和蜥蜴再生尾巴的稀疏粒细胞中

IF 1.1 4区 生物学 Q4 ANATOMY & MORPHOLOGY
Acta Zoologica Pub Date : 2021-11-26 DOI:10.1111/azo.12413
Lorenzo Alibardi
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引用次数: 0

摘要

显微镜显示谷胱甘肽S-转移酶储存在骨髓中的大颗粒髓细胞和蜥蜴再生尾巴的稀疏粒细胞中。动物学报(斯德哥尔摩)。蜥蜴的尾巴再生发生在免疫特权芽基后,在各种免疫抑制细胞和生物分子保持低炎症的地方形成。限制炎症的分子可能包括谷胱甘肽和谷胱甘肽S-转移酶(GST)。这些分子减少活性氧化物质(ROS)并解毒外来分子。在本研究中,已经分析了在蜥蜴Podarcis muralis尾巴再生的早期阶段GST的存在。利用兔多克隆抗体,针对村鼠GST中存在的表位,进行了光镜和电子显微镜免疫组织化学和蛋白质印迹研究。该研究在尾椎骨骨髓中的早幼粒细胞和骨髓细胞中检测到25和32kDa的蛋白质,这些细胞可能具有嗜碱性或嗜酸性。这些细胞仍在增殖,并产生嗜碱性或嗜酸性粒细胞,这些粒细胞也迁移到再生的尾部。储存在早幼粒细胞和骨髓细胞颗粒中的酶也定位在再生芽母细胞锥中的粒细胞细胞质中,嗜碱性或/和嗜酸性。有人认为,芽基中的这些粒细胞可以减少ROS并解毒潜在的炎症代谢产物,与其他分子一起限制炎症并促进再生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microscopy suggests that glutathione S-transferase is stored in large granules of myeloid cells in bone marrow and sparse granulocytes of the regenerating tail of lizard

Microscopy suggests that glutathione S-transferase is stored in large granules of myeloid cells in bone marrow and sparse granulocytes of the regenerating tail of lizard. Acta Zoologica (Stockolm). Tail regeneration in lizards occurs after an immune-privileged blastema and is formed where various immunosuppressive cells and biomolecules keep inflammation low. Molecules that limit inflammation may include glutathione and glutathione S-transferase (GST). These molecules reduce reactive oxidative species (ROS) and detoxify extraneous molecules. In the present study, the presence of GST has been analysed in the early phases of tail regeneration in the lizard Podarcis muralis. Using a rabbit polyclonal antibody directed against epitopes present in GST of Pmuralis, a light and electron microscopy immunohistochemical and Western blotting study has been done. The study detected proteins of 25 and 32 kDa in pale-medium electron-dense and large granules present in promyelocytes and myelocytes of likely basophilic or eosinophilic fate that is present in the bone marrow of tail vertebrae. These cells are still proliferating and give rise to basophilic or eosinophilic granulocytes that also migrate into the regenerating tail. The enzyme stored in granules of promyelocytes and myelocytes is also localized in the cytoplasm of granulocytes present in the regenerating blastema-cone, basophilic or/and eosinophilic. It is suggested that these granulocytes in the blastema reduce ROS and detoxify potentially inflammatory metabolites, contributing with other molecules to limit inflammation and favour regeneration.

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来源期刊
Acta Zoologica
Acta Zoologica 生物-动物学
CiteScore
2.90
自引率
8.30%
发文量
35
审稿时长
>12 weeks
期刊介绍: Published regularly since 1920, Acta Zoologica has retained its position as one of the world''s leading journals in the field of animal organization, development, structure and function. Each issue publishes original research of interest to zoologists and physiologists worldwide, in the field of animal structure (from the cellular to the organismic level) and development with emphasis on functional, comparative and phylogenetic aspects. Occasional review articles are also published, as well as book reviews.
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