Birgit Wassermann, Tomislav Cernava, Simon Goertz, Jennifer Zur, Steffen Rietz, Isabella Kögl, Amine Abbadi, Gabriele Berg
{"title":"低氮施肥丰富了芸苔属后代种子微生物组中的固氮细菌","authors":"Birgit Wassermann, Tomislav Cernava, Simon Goertz, Jennifer Zur, Steffen Rietz, Isabella Kögl, Amine Abbadi, Gabriele Berg","doi":"10.1002/sae2.12046","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Introduction</h3>\n \n <p>The consequences of overfertilization on agricultural and native ecosystems are well-documented, but the impact of reduced fertilization on plant–microbe interactions is less understood. Here, we analysed the impact of different nitrogen (N) fertilization regimes over two subsequent growing seasons on the seed microbiome of winter oilseed rape (<i>Brassica napus</i> L.).</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>Seed microbial diversity, composition, and abundance were analysed by a combined approach of amplicon sequencing, quantitative real-time PCR, and microscopy. A germination assay was conducted to quantify bacteria and <i>nifH</i> genes in roots.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Two consecutive years of either low or high N fertilization resulted in a significant shift in seed microbiota; nine discriminating bacterial orders and 32 bacterial features were identified. Among them, potentially N-fixing bacteria (Rhizobiales and <i>Rhizobium</i>) were identified as biomarkers for low N fertilization. Moreover, quantitative measurements revealed that 16S ribosomal RNA (rRNA) and bacterial <i>nifH</i> gene counts were significantly higher under lower N availability. Relative to total 16S rRNA genes, seeds and roots contained 0.002% and 0.3% <i>nifH</i> genes on average, respectively. The alternation of N levels between the first and the second growing season (high to low, or low to high) had, however, no impact on seed microbiota, suggesting that a low N availability for at least two generations is required to enrich N-fixing taxa in seeds.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Our study revealed a flexible, environmentally influenced seed microbiome assembly and suggests innovative microbiome management via biological nitrogen fixation to optimize fertilization regimes in future agriculture.</p>\n </section>\n </div>","PeriodicalId":100834,"journal":{"name":"Journal of Sustainable Agriculture and Environment","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12046","citationCount":"0","resultStr":"{\"title\":\"Low nitrogen fertilization enriches nitrogen-fixing bacteria in the Brassica seed microbiome of subsequent generations\",\"authors\":\"Birgit Wassermann, Tomislav Cernava, Simon Goertz, Jennifer Zur, Steffen Rietz, Isabella Kögl, Amine Abbadi, Gabriele Berg\",\"doi\":\"10.1002/sae2.12046\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Introduction</h3>\\n \\n <p>The consequences of overfertilization on agricultural and native ecosystems are well-documented, but the impact of reduced fertilization on plant–microbe interactions is less understood. Here, we analysed the impact of different nitrogen (N) fertilization regimes over two subsequent growing seasons on the seed microbiome of winter oilseed rape (<i>Brassica napus</i> L.).</p>\\n </section>\\n \\n <section>\\n \\n <h3> Materials and Methods</h3>\\n \\n <p>Seed microbial diversity, composition, and abundance were analysed by a combined approach of amplicon sequencing, quantitative real-time PCR, and microscopy. A germination assay was conducted to quantify bacteria and <i>nifH</i> genes in roots.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Two consecutive years of either low or high N fertilization resulted in a significant shift in seed microbiota; nine discriminating bacterial orders and 32 bacterial features were identified. Among them, potentially N-fixing bacteria (Rhizobiales and <i>Rhizobium</i>) were identified as biomarkers for low N fertilization. Moreover, quantitative measurements revealed that 16S ribosomal RNA (rRNA) and bacterial <i>nifH</i> gene counts were significantly higher under lower N availability. Relative to total 16S rRNA genes, seeds and roots contained 0.002% and 0.3% <i>nifH</i> genes on average, respectively. The alternation of N levels between the first and the second growing season (high to low, or low to high) had, however, no impact on seed microbiota, suggesting that a low N availability for at least two generations is required to enrich N-fixing taxa in seeds.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>Our study revealed a flexible, environmentally influenced seed microbiome assembly and suggests innovative microbiome management via biological nitrogen fixation to optimize fertilization regimes in future agriculture.</p>\\n </section>\\n </div>\",\"PeriodicalId\":100834,\"journal\":{\"name\":\"Journal of Sustainable Agriculture and Environment\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-05-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/sae2.12046\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Sustainable Agriculture and Environment\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/sae2.12046\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Sustainable Agriculture and Environment","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/sae2.12046","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Low nitrogen fertilization enriches nitrogen-fixing bacteria in the Brassica seed microbiome of subsequent generations
Introduction
The consequences of overfertilization on agricultural and native ecosystems are well-documented, but the impact of reduced fertilization on plant–microbe interactions is less understood. Here, we analysed the impact of different nitrogen (N) fertilization regimes over two subsequent growing seasons on the seed microbiome of winter oilseed rape (Brassica napus L.).
Materials and Methods
Seed microbial diversity, composition, and abundance were analysed by a combined approach of amplicon sequencing, quantitative real-time PCR, and microscopy. A germination assay was conducted to quantify bacteria and nifH genes in roots.
Results
Two consecutive years of either low or high N fertilization resulted in a significant shift in seed microbiota; nine discriminating bacterial orders and 32 bacterial features were identified. Among them, potentially N-fixing bacteria (Rhizobiales and Rhizobium) were identified as biomarkers for low N fertilization. Moreover, quantitative measurements revealed that 16S ribosomal RNA (rRNA) and bacterial nifH gene counts were significantly higher under lower N availability. Relative to total 16S rRNA genes, seeds and roots contained 0.002% and 0.3% nifH genes on average, respectively. The alternation of N levels between the first and the second growing season (high to low, or low to high) had, however, no impact on seed microbiota, suggesting that a low N availability for at least two generations is required to enrich N-fixing taxa in seeds.
Conclusion
Our study revealed a flexible, environmentally influenced seed microbiome assembly and suggests innovative microbiome management via biological nitrogen fixation to optimize fertilization regimes in future agriculture.