Cover Feature: Isothermal Titration Calorimetry for Characterizing the Zinc(II)-Binding Properties of Photocaged Complexes with Micromolar Affinity (Anal. Sens. 2/2023)

The cover feature image shows alternate methods to characterize photocages by illustrating the photolysis of the bis-complex of the PyrDeCage, a low affinity photocage for divalent zinc. The uncaging involves a photodecarboxylation of a meta-nitrophenylacetic acid caging group to make weakly binding zinc ligands. The zinc release can be useful for investigating the impact of zinc ions in biology or other areas. The characterization of the zinc-binding properties required exploring new methodologies including isothermal titration calorimetry that uses thermodynamic data to provide binding constants. Cover designed by Chelsea A. Barr. More information can be found in the Research Article by Shawn C. Burdette and co-workers.