{"title":"生物合成木质素脱氢聚合物制备透明质酸混合水凝胶复合材料用于软骨修复","authors":"Wenhui Pei, Yalikun Yusufu, Yifei Zhan, Xucai Wang, Jian Gan, Liming Zheng, Peng Wang, Kai Zhang, Caoxing Huang","doi":"10.1007/s42114-023-00758-6","DOIUrl":null,"url":null,"abstract":"<div><p>Lignin possesses a number of functional groups including phenolic hydroxyl and methoxy groups, which grant its bioactivity for the fabrication of bio-polymer-based composites in bone tissue engineering applications. However, the heterogeneity of natural lignin limits its use in biomedicine. In the present study, a bio-enzyme approach was proposed to synthesize lignin-dehydrogenated polymers from the precursors of arabinogalactan (DHP-A) and xylose (DHP-X), which possess more homogeneous substructures with appropriate functional groups. Both DHP-A and DHP-X showed excellent in vitro abilities for regulating biocompatibility, “pre-oxidation,” and chondrogenic differentiation, in which DHP-A possessed cartilage repair ability due to its abundant content of phenolic hydroxyl groups (3.00 mmol g<sup>−1</sup>). Hence, DHP-A was hybridized with hyaluronic acid (HA) to prepare a hydrogel (DHP-HA) composite, which exhibited the compressive strength and modulus of 810 kPa and 310 kPa, respectively. Notably, these properties closely resemble those of articular cartilage, which typically ranges from 320 to 810 kPa. The application of DHP-HA hydrogel composite in a rat cartilage defect model in vivo revealed that it promoted the regeneration of hyaline cartilage rather than hypertrophic cartilage, which could heal 66.22–79.26% of the cartilage defects compared to the control group. Pre-oxidation of DHP-A elicits a mechanism that activates the oxidative stress system, leading to an augmented stress response and consequent increase in stress resistance. This study introduces a pioneering enzymatic synthesis technique to prepare the biologically active lignin for creating bio-polymer-based composites, demonstrating its potential as an innovative avenue for therapeutic cartilage regeneration.</p><h3>Graphical Abstract</h3><p>Bioenzymatically synthesized lignin dehydrogenation polymers to hybridize with hyaluronic acid to prepare hydrogel composites for promoting cartilage defect repair.</p>\n <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\n </div>","PeriodicalId":7220,"journal":{"name":"Advanced Composites and Hybrid Materials","volume":"6 5","pages":""},"PeriodicalIF":23.2000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":"{\"title\":\"Biosynthesizing lignin dehydrogenation polymer to fabricate hybrid hydrogel composite with hyaluronic acid for cartilage repair\",\"authors\":\"Wenhui Pei, Yalikun Yusufu, Yifei Zhan, Xucai Wang, Jian Gan, Liming Zheng, Peng Wang, Kai Zhang, Caoxing Huang\",\"doi\":\"10.1007/s42114-023-00758-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Lignin possesses a number of functional groups including phenolic hydroxyl and methoxy groups, which grant its bioactivity for the fabrication of bio-polymer-based composites in bone tissue engineering applications. However, the heterogeneity of natural lignin limits its use in biomedicine. In the present study, a bio-enzyme approach was proposed to synthesize lignin-dehydrogenated polymers from the precursors of arabinogalactan (DHP-A) and xylose (DHP-X), which possess more homogeneous substructures with appropriate functional groups. Both DHP-A and DHP-X showed excellent in vitro abilities for regulating biocompatibility, “pre-oxidation,” and chondrogenic differentiation, in which DHP-A possessed cartilage repair ability due to its abundant content of phenolic hydroxyl groups (3.00 mmol g<sup>−1</sup>). Hence, DHP-A was hybridized with hyaluronic acid (HA) to prepare a hydrogel (DHP-HA) composite, which exhibited the compressive strength and modulus of 810 kPa and 310 kPa, respectively. Notably, these properties closely resemble those of articular cartilage, which typically ranges from 320 to 810 kPa. The application of DHP-HA hydrogel composite in a rat cartilage defect model in vivo revealed that it promoted the regeneration of hyaline cartilage rather than hypertrophic cartilage, which could heal 66.22–79.26% of the cartilage defects compared to the control group. Pre-oxidation of DHP-A elicits a mechanism that activates the oxidative stress system, leading to an augmented stress response and consequent increase in stress resistance. This study introduces a pioneering enzymatic synthesis technique to prepare the biologically active lignin for creating bio-polymer-based composites, demonstrating its potential as an innovative avenue for therapeutic cartilage regeneration.</p><h3>Graphical Abstract</h3><p>Bioenzymatically synthesized lignin dehydrogenation polymers to hybridize with hyaluronic acid to prepare hydrogel composites for promoting cartilage defect repair.</p>\\n <div><figure><div><div><picture><source><img></source></picture></div></div></figure></div>\\n </div>\",\"PeriodicalId\":7220,\"journal\":{\"name\":\"Advanced Composites and Hybrid Materials\",\"volume\":\"6 5\",\"pages\":\"\"},\"PeriodicalIF\":23.2000,\"publicationDate\":\"2023-09-30\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"2\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Advanced Composites and Hybrid Materials\",\"FirstCategoryId\":\"88\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s42114-023-00758-6\",\"RegionNum\":2,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MATERIALS SCIENCE, COMPOSITES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Composites and Hybrid Materials","FirstCategoryId":"88","ListUrlMain":"https://link.springer.com/article/10.1007/s42114-023-00758-6","RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MATERIALS SCIENCE, COMPOSITES","Score":null,"Total":0}
Biosynthesizing lignin dehydrogenation polymer to fabricate hybrid hydrogel composite with hyaluronic acid for cartilage repair
Lignin possesses a number of functional groups including phenolic hydroxyl and methoxy groups, which grant its bioactivity for the fabrication of bio-polymer-based composites in bone tissue engineering applications. However, the heterogeneity of natural lignin limits its use in biomedicine. In the present study, a bio-enzyme approach was proposed to synthesize lignin-dehydrogenated polymers from the precursors of arabinogalactan (DHP-A) and xylose (DHP-X), which possess more homogeneous substructures with appropriate functional groups. Both DHP-A and DHP-X showed excellent in vitro abilities for regulating biocompatibility, “pre-oxidation,” and chondrogenic differentiation, in which DHP-A possessed cartilage repair ability due to its abundant content of phenolic hydroxyl groups (3.00 mmol g−1). Hence, DHP-A was hybridized with hyaluronic acid (HA) to prepare a hydrogel (DHP-HA) composite, which exhibited the compressive strength and modulus of 810 kPa and 310 kPa, respectively. Notably, these properties closely resemble those of articular cartilage, which typically ranges from 320 to 810 kPa. The application of DHP-HA hydrogel composite in a rat cartilage defect model in vivo revealed that it promoted the regeneration of hyaline cartilage rather than hypertrophic cartilage, which could heal 66.22–79.26% of the cartilage defects compared to the control group. Pre-oxidation of DHP-A elicits a mechanism that activates the oxidative stress system, leading to an augmented stress response and consequent increase in stress resistance. This study introduces a pioneering enzymatic synthesis technique to prepare the biologically active lignin for creating bio-polymer-based composites, demonstrating its potential as an innovative avenue for therapeutic cartilage regeneration.
Graphical Abstract
Bioenzymatically synthesized lignin dehydrogenation polymers to hybridize with hyaluronic acid to prepare hydrogel composites for promoting cartilage defect repair.
期刊介绍：
Advanced Composites and Hybrid Materials is a leading international journal that promotes interdisciplinary collaboration among materials scientists, engineers, chemists, biologists, and physicists working on composites, including nanocomposites. Our aim is to facilitate rapid scientific communication in this field.
The journal publishes high-quality research on various aspects of composite materials, including materials design, surface and interface science/engineering, manufacturing, structure control, property design, device fabrication, and other applications. We also welcome simulation and modeling studies that are relevant to composites. Additionally, papers focusing on the relationship between fillers and the matrix are of particular interest.
Our scope includes polymer, metal, and ceramic matrices, with a special emphasis on reviews and meta-analyses related to materials selection. We cover a wide range of topics, including transport properties, strategies for controlling interfaces and composition distribution, bottom-up assembly of nanocomposites, highly porous and high-density composites, electronic structure design, materials synergisms, and thermoelectric materials.
Advanced Composites and Hybrid Materials follows a rigorous single-blind peer-review process to ensure the quality and integrity of the published work.
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