生物化学和代谢特征是布甘肠杆菌WRS7†干旱适应的基础

IF 3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular omics Pub Date : 2023-05-29 DOI:10.1039/D3MO00051F
Saumya Arora, Piyoosh K Babele and Prabhat Nath Jha
{"title":"生物化学和代谢特征是布甘肠杆菌WRS7†干旱适应的基础","authors":"Saumya Arora, Piyoosh K Babele and Prabhat Nath Jha","doi":"10.1039/D3MO00051F","DOIUrl":null,"url":null,"abstract":"<p >Drought alone causes more annual loss in crop yield than the sum of all other environmental stresses. There is growing interest in harnessing the potential of stress-resilient PGPR in conferring plant resistance and enhancing crop productivity in drought-affected agroecosystems. A detailed understanding of the complex physiological and biochemical responses will open up the avenues to stress adaptation mechanisms of PGPR communities under drought. It will pave the way for rhizosphere engineering through metabolically engineered PGPR. Therefore, to reveal the physiological and metabolic networks in response to drought-mediated osmotic stress, we performed biochemical analyses and applied untargeted metabolomics to investigate the stress adaptation mechanisms of a PGPR <em>Enterobacter bugendensis</em> WRS7 (<em>Eb</em> WRS7). Drought caused oxidative stress and resulted in slower growth rates in <em>Eb</em> WRS7. However, <em>Eb</em> WRS7 could tolerate drought stress and did not show changes in cell morphology under stress conditions. Overproduction of ROS caused lipid peroxidation (increment in MDA) and eventually activated antioxidant systems and cell signalling cascades, which led to the accumulation of ions (Na<small><sup>+</sup></small>, K<small><sup>+</sup></small>, and Ca<small><sup>2+</sup></small>), osmolytes (proline, exopolysaccharides, betaine, and trehalose), and modulated lipid dynamics of the plasma membranes for osmosensing and osmoregulation, suggesting an osmotic stress adaption mechanism in PGPR <em>Eb</em> WRS7. Finally, GC–MS-based metabolite profiling and deregulated metabolic responses highlighted the role of osmolytes, ions, and intracellular metabolites in regulating <em>Eb</em> WRS7 metabolism. Our results suggest that understanding the role of metabolites and metabolic pathways can be exploited for future metabolic engineering of PGPR and developing bio inoculants for plant growth promotion under drought-affected agroecosystems.</p>","PeriodicalId":19065,"journal":{"name":"Molecular omics","volume":" 8","pages":" 640-652"},"PeriodicalIF":3.0000,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Biochemical and metabolic signatures are fundamental to drought adaptation in PGPR Enterobacter bugandensis WRS7†\",\"authors\":\"Saumya Arora, Piyoosh K Babele and Prabhat Nath Jha\",\"doi\":\"10.1039/D3MO00051F\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Drought alone causes more annual loss in crop yield than the sum of all other environmental stresses. There is growing interest in harnessing the potential of stress-resilient PGPR in conferring plant resistance and enhancing crop productivity in drought-affected agroecosystems. A detailed understanding of the complex physiological and biochemical responses will open up the avenues to stress adaptation mechanisms of PGPR communities under drought. It will pave the way for rhizosphere engineering through metabolically engineered PGPR. Therefore, to reveal the physiological and metabolic networks in response to drought-mediated osmotic stress, we performed biochemical analyses and applied untargeted metabolomics to investigate the stress adaptation mechanisms of a PGPR <em>Enterobacter bugendensis</em> WRS7 (<em>Eb</em> WRS7). Drought caused oxidative stress and resulted in slower growth rates in <em>Eb</em> WRS7. However, <em>Eb</em> WRS7 could tolerate drought stress and did not show changes in cell morphology under stress conditions. Overproduction of ROS caused lipid peroxidation (increment in MDA) and eventually activated antioxidant systems and cell signalling cascades, which led to the accumulation of ions (Na<small><sup>+</sup></small>, K<small><sup>+</sup></small>, and Ca<small><sup>2+</sup></small>), osmolytes (proline, exopolysaccharides, betaine, and trehalose), and modulated lipid dynamics of the plasma membranes for osmosensing and osmoregulation, suggesting an osmotic stress adaption mechanism in PGPR <em>Eb</em> WRS7. Finally, GC–MS-based metabolite profiling and deregulated metabolic responses highlighted the role of osmolytes, ions, and intracellular metabolites in regulating <em>Eb</em> WRS7 metabolism. Our results suggest that understanding the role of metabolites and metabolic pathways can be exploited for future metabolic engineering of PGPR and developing bio inoculants for plant growth promotion under drought-affected agroecosystems.</p>\",\"PeriodicalId\":19065,\"journal\":{\"name\":\"Molecular omics\",\"volume\":\" 8\",\"pages\":\" 640-652\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2023-05-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular omics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2023/mo/d3mo00051f\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular omics","FirstCategoryId":"99","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2023/mo/d3mo00051f","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

仅干旱一项造成的作物年产量损失就超过所有其他环境压力的总和。在受干旱影响的农业生态系统中,利用抗逆性PGPR的潜力赋予植物抗性和提高作物生产力的兴趣日益增加。详细了解其复杂的生理生化反应,将为探究PGPR群落在干旱条件下的胁迫适应机制开辟道路。它将通过代谢工程的PGPR为根际工程铺平道路。因此,为了揭示干旱介导的渗透胁迫下的生理和代谢网络,我们进行了生化分析并应用非靶向代谢组学研究了PGPR bugendensis肠杆菌WRS7 (Eb WRS7)的胁迫适应机制。干旱引起氧化应激,导致Eb WRS7生长速度减慢。然而,Eb WRS7能够耐受干旱胁迫,在干旱条件下细胞形态没有变化。ROS的过量产生导致脂质过氧化(MDA增加),并最终激活抗氧化系统和细胞信号级联反应,导致离子(Na+、K+和Ca2+)、渗透物(脯氨酸、外多糖、甜菜碱和海藻糖)的积累,并调节质膜的脂质动力学,以进行渗透传感和渗透调节,表明PGPR Eb WRS7中存在渗透胁迫适应机制。最后,基于gc - ms的代谢物分析和解除管制的代谢反应强调了渗透物、离子和细胞内代谢物在调节Eb WRS7代谢中的作用。我们的研究结果表明,了解代谢物的作用和代谢途径可以为未来的PGPR代谢工程和开发促进干旱农业生态系统下植物生长的生物接种剂提供基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Biochemical and metabolic signatures are fundamental to drought adaptation in PGPR Enterobacter bugandensis WRS7†

Biochemical and metabolic signatures are fundamental to drought adaptation in PGPR Enterobacter bugandensis WRS7†

Drought alone causes more annual loss in crop yield than the sum of all other environmental stresses. There is growing interest in harnessing the potential of stress-resilient PGPR in conferring plant resistance and enhancing crop productivity in drought-affected agroecosystems. A detailed understanding of the complex physiological and biochemical responses will open up the avenues to stress adaptation mechanisms of PGPR communities under drought. It will pave the way for rhizosphere engineering through metabolically engineered PGPR. Therefore, to reveal the physiological and metabolic networks in response to drought-mediated osmotic stress, we performed biochemical analyses and applied untargeted metabolomics to investigate the stress adaptation mechanisms of a PGPR Enterobacter bugendensis WRS7 (Eb WRS7). Drought caused oxidative stress and resulted in slower growth rates in Eb WRS7. However, Eb WRS7 could tolerate drought stress and did not show changes in cell morphology under stress conditions. Overproduction of ROS caused lipid peroxidation (increment in MDA) and eventually activated antioxidant systems and cell signalling cascades, which led to the accumulation of ions (Na+, K+, and Ca2+), osmolytes (proline, exopolysaccharides, betaine, and trehalose), and modulated lipid dynamics of the plasma membranes for osmosensing and osmoregulation, suggesting an osmotic stress adaption mechanism in PGPR Eb WRS7. Finally, GC–MS-based metabolite profiling and deregulated metabolic responses highlighted the role of osmolytes, ions, and intracellular metabolites in regulating Eb WRS7 metabolism. Our results suggest that understanding the role of metabolites and metabolic pathways can be exploited for future metabolic engineering of PGPR and developing bio inoculants for plant growth promotion under drought-affected agroecosystems.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Molecular omics
Molecular omics Biochemistry, Genetics and Molecular Biology-Biochemistry
CiteScore
5.40
自引率
3.40%
发文量
91
期刊介绍: Molecular Omics publishes high-quality research from across the -omics sciences. Topics include, but are not limited to: -omics studies to gain mechanistic insight into biological processes – for example, determining the mode of action of a drug or the basis of a particular phenotype, such as drought tolerance -omics studies for clinical applications with validation, such as finding biomarkers for diagnostics or potential new drug targets -omics studies looking at the sub-cellular make-up of cells – for example, the subcellular localisation of certain proteins or post-translational modifications or new imaging techniques -studies presenting new methods and tools to support omics studies, including new spectroscopic/chromatographic techniques, chip-based/array technologies and new classification/data analysis techniques. New methods should be proven and demonstrate an advance in the field. Molecular Omics only accepts articles of high importance and interest that provide significant new insight into important chemical or biological problems. This could be fundamental research that significantly increases understanding or research that demonstrates clear functional benefits. Papers reporting new results that could be routinely predicted, do not show a significant improvement over known research, or are of interest only to the specialist in the area are not suitable for publication in Molecular Omics.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信