管状蜘蛛蛋白重复结构域的1H, 15N和13C共振分配2

IF 0.8 4区生物学 Q4 BIOPHYSICS

Biomolecular NMR Assignments Pub Date : 2021-08-26 DOI:10.1007/s12104-021-10048-1

Tiantian Fan, Yan Zhang, Jing-Song Fan, Wensu Yuan, Zhi Lin

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引用次数: 1

摘要

蜘蛛丝以其优异的机械性能而闻名。在由不同腹部腺体产生的六种丝和一种丝胶中，管状(卵状)丝的独特之处在于它的丝氨酸含量高，甘氨酸含量低。卵丝是由至少两个蛛纲，管状蛛纲1 (TuSp1)和TuSp2纺成的。TuSp1和TuSp2分别被鉴定为管状腺的主要成分和次要成分。TuSp2由多个末端尾巴短的重复结构域组成，与所有已知的蜘蛛蛋白具有非常有限的同源性。在这里，我们报道了TuSp2-RP的主链和侧链共振分配，作为研究卵丝形成的结构和功能的基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

1H, 15N and 13C resonance assignments of a repetitive domain of tubuliform spidroin 2

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1H, 15N and 13C resonance assignments of a repetitive domain of tubuliform spidroin 2

Spider silk is renowned for its excellent mechanical properties. Among six types of silk and one silk glue produced by different abdominal glands for various purposes, tubuliform (eggcase) silk is unique due to its high serine and low glycine content. Eggcase silk is spun from at least two spidroins, tubuliform spidroin 1 (TuSp1) and TuSp2. TuSp1 and TuSp2 were identified as the major and the minor components in tubuliform glands, respectively. TuSp2 consists of multiple repetitive (RP) domains with short terminal tails and shares very limited homology to all known spidroins. Here we report backbone and side chain resonance assignments of TuSp2-RP as a basis for structural and functional studies on eggcase silk formation.

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来源期刊

Biomolecular NMR Assignments 生物-光谱学

CiteScore

1.70

自引率

11.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Biomolecular NMR Assignments provides a forum for publishing sequence-specific resonance assignments for proteins and nucleic acids as Assignment Notes. Chemical shifts for NMR-active nuclei in macromolecules contain detailed information on molecular conformation and properties. Publication of resonance assignments in Biomolecular NMR Assignments ensures that these data are deposited into a public database at BioMagResBank (BMRB; http://www.bmrb.wisc.edu/), where they are available to other researchers. Coverage includes proteins and nucleic acids; Assignment Notes are processed for rapid online publication and are published in biannual online editions in June and December.