由PHBV、PCL及其75:25混合物组成的致密、多孔和纤维状支架:体外形态学和细胞化学表征

IF 1.7 4区 工程技术 Q4 POLYMER SCIENCE
Larissa Pereira Modolo , Wellington Raimundo França , Márcia M.O. Simbara , Sonia M. Malmonge , Arnaldo R. Santos Jr.
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引用次数: 3

摘要

在组织工程中,生物可吸收聚合物通常用于模拟受损组织的特征。尽管如此,我们没有发现同一细胞对致密、多孔和纤维生物材料反应的比较研究。本工作旨在研究纯聚合物[聚(ε-己内酯)(PCL)和聚(羟基丁酸酯-共-羟基戊酸酯)(PHBV)]及其75:25在成纤维细胞系Vero细胞中以致密、多孔和纤维形式的共混物的体外研究。用扫描电子显微镜(SEM)对生物材料进行了形态学表征。MTT法用于分析可能的细胞毒性。Vero细胞在生物材料上培养120 h,并进行SEM、形态学和细胞化学分析。所使用的染料是结晶紫(形态)、pH 4.0(用于核酸和糖胺聚糖)和2.5(仅用于糖胺聚糖的)的甲苯胺蓝、碘酸Schiff反应性染料(用于中性糖的)、pH 2.5(用于总蛋白的)的木糖核酸酶-胭脂红和苦苷/苏木精(用于选择性胶原蛋白分析)。所研究的生物材料不被认为具有细胞毒性。细胞能够粘附在基质上并在基质上增殖。细胞化学数据表明,细胞具有高功能活性,在支架上具有高蛋白质合成,但不积累酸性或中性糖。纤维聚合物上的细胞看起来更细长,遵循纤维的形状。关于支架形式(多孔或纤维),体外数据并没有显示出一种类型相对于另一种类型的巨大优势。然后,所有测试的聚合物都适用于组织工程。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dense, porous, and fibrous scaffolds composed of PHBV, PCL, and their 75:25 blend: an in vitro morphological and cytochemical characterization

In tissue engineering bioresorbable polymers are commonly used to mimetize a damaged tissue characteristics. In spite of that, we did not find a comparative study of the response of the same cell to dense, porous, and fibrous biomaterials. The present work aimed to study the in vitro of pure polymers [poly (ε-caprolactone) (PCL) and poly (hydroxybutyrate-co-hydroxyvalerate) (PHBV)] and their 75:25 blend in dense, porous, and fibrous forms in Vero cells, a fibroblast cell line. The biomaterials were characterized morphologically by scanning electron microscopy (SEM). The MTT assay was used for analysis of possible cytotoxicity. Vero cells were cultured on biomaterials for 120 h and submitted to SEM, morphological and cytochemical analyses. The dyes used were crystal violet (morphology), toluidine blue at pH 4.0 (for nucleic acids and glycosaminoglycans) and 2.5 (for glycosaminoglycans only), periodic acid-Schiff reactive (for neutral sugars), xylidine ponceau at pH 2.5 (for total proteins), and picrosirius/hematoxylin (for selective collagen analysis). The biomaterials studied were not considered citocytotoxic. The cells were able to adhere to and proliferate on the substrates. The cytochemical data indicate that the cells have high functional activity, with high protein synthesis on the scaffolds but do not accumulate acid or neutral sugars. The cells on the fibrous polymers appear to be more elongated, following the shape of the fibers. Regarding the scaffold form (porous or fibrous), the in vitro data do not show great superiority of one type to the other. Then, all the polymers tested are suitable for tissue engineering.

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来源期刊
CiteScore
3.50
自引率
5.30%
发文量
37
审稿时长
1.6 months
期刊介绍: The scope of the journal is to publish original contributions and reviews on studies, methodologies, instrumentation, and applications involving the analysis and characterization of polymers and polymeric-based materials, including synthetic polymers, blends, composites, fibers, coatings, supramolecular structures, polysaccharides, and biopolymers. The Journal will accept papers and review articles on the following topics and research areas involving fundamental and applied studies of polymer analysis and characterization: Characterization and analysis of new and existing polymers and polymeric-based materials. Design and evaluation of analytical instrumentation and physical testing equipment. Determination of molecular weight, size, conformation, branching, cross-linking, chemical structure, and sequence distribution. Using separation, spectroscopic, and scattering techniques. Surface characterization of polymeric materials. Measurement of solution and bulk properties and behavior of polymers. Studies involving structure-property-processing relationships, and polymer aging. Analysis of oligomeric materials. Analysis of polymer additives and decomposition products.
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