长时间间歇性禁食对自噬、炎性体和衰老基因表达的影响:一项健康年轻男性的探索性研究

IF 1.9 Q3 ENDOCRINOLOGY & METABOLISM
Zulrahman Erlangga , Samaneh Khosandam Ghashang , Imad Hamdan , Anette Melk , Christoph Gutenbrunner , Boya Nugraha
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引用次数: 1

摘要

间歇性禁食(IF)通过自噬和炎症活性降低与寿命和健康相关。衰老过程与自噬活动有关,并决定了器官的实际年龄。延长的IF对自噬、炎性体活性和衰老的影响有待进一步阐明。招募25名健康的年轻男性,禁食17-19小时,持续30天。在IF开始前1周(TP1)、开始后2周(TP2)、开始后1个月(TP3)、结束后1周(TP4)采集血样,提取mRNA, qPCR检测自噬(ATG5、ULK1、BECN1)、炎性小体(NLRP3、IL-1 β、ASC、TNF- α)、衰老(p16INK4A、p21、P53)标志物表达水平。延长IF可诱导ATG5、ULK1和BECN1在TP2的表达水平,而在TP4的表达水平降低。NLRP3和IL-1β表达水平在TP2和TP3时升高,而在TP4时降低。ASC表达水平在TP2时升高,在TP3时降低,在TP4时恢复正常。延长IF持续降低TNF-α表达水平至TP4。随着观察时间的延长,p16INK4A和p21的表达水平有降低的趋势。P53在TP2和TP3的表达水平升高,而在TP4的表达水平下降。我们的研究表明,延长的IF以一种时间依赖性的方式影响自噬、炎性体和衰老的活动。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effect of prolonged intermittent fasting on autophagy, inflammasome and senescence genes expressions: An exploratory study in healthy young males

Intermittent fasting (IF) has been associated with longevity and healthspan through autophagy and reduction of inflammation activity. The senescence process is linked with autophagy activity and determines the organ age as chronological age. The effect of prolonged IF on autophagy, inflammasome activity, and senescence needs to be elucidated further. Twenty-five healthy young males were recruited and performed 17–19 h/day fasting for 30 days. Blood samples were collected one week before (TP1), two weeks after the start (TP2), one month after the start (TP3), and one week after the end (TP4) of the IF and extracted to obtain mRNA to determine autophagy (ATG5, ULK1, and BECN1), inflammasome (NLRP3, IL-1 β, ASC, and TNF- α), and senescence (p16INK4A, p21, and P53) marker expression level by qPCR. Prolonged IF induced expression level of ATG5, ULK1 and BECN1 at TP2 but decreased at TP4. The NLRP3 and IL-1β expression level increased at TP2 and TP3, but decreased at TP4. ASC expression level increased at TP2, decreased at TP3 and returned to normal at TP4. Prolonged IF kept reducing TNF-α expression level until TP4. The expression level of p16INK4A and p21 tended to decrease over the time of observation. The expression level of P53 increased at TP2 and TP3 but decreased at TP4. Our study showed that prolonged IF affects the activities of autophagy, inflammasome, and senescence in a time-dependent manner.

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来源期刊
Human Nutrition and Metabolism
Human Nutrition and Metabolism Agricultural and Biological Sciences-Food Science
CiteScore
1.50
自引率
0.00%
发文量
30
审稿时长
188 days
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