产纤维素酶微生物联合体预处理提高稻草沼气产量

IF 8 Q1 ENERGY & FUELS
Sahil Sahil , Palanisamy Karvembu , Ravneet Kaur , Priya Katyal , Urmila Gupta Phutela
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引用次数: 0

摘要

稻草(RS)是一种丰富的木质纤维素生物质,可用于通过厌氧消化产生沼气。然而,全纤维素复合体周围的木质素-二氧化硅结垢阻碍了其消化。因此,在本研究中,用旁遮普农业大学的微生物群落(PAU分解器)对RS进行了生物预处理,该微生物群落由芽孢杆菌属、德尔夫蒂亚属、假单胞菌属、赖氨芽孢杆菌梭形体、烟草节杆菌属、艾希门菌属、曲霉属和木霉属组成,牛粪沼气厂产生的未充分利用的废垃圾被用作群落大规模繁殖的生长介质。浸泡和切碎的RS用一式三份的联合培养物和单个培养物预处理5天和10天,并分析生化参数和纤维素分解活性。将经预处理的秸秆用于2L容量厌氧消化器中的沼气生产。与未处理的RS相比,联合体处理的RS的细胞裂解活性,即内葡聚糖酶(0.071 IU/mL)、外葡聚糖酶(0.027 IU/mL。这可能归因于PAU分解器的协同纤维素分解活性,从而提高了RS的沼气产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Enhanced biogas production from rice straw through pretreatment with cellulase producing microbial consortium

Enhanced biogas production from rice straw through pretreatment with cellulase producing microbial consortium

Rice straw (RS) is a rich lignocellulosic biomass that can be employed for biogas generation through anaerobic digestion. However, lignin-silica encrustation around the holocellulose complex hinders its digestibility. Therefore, in this present study, RS was biologically pretreated with microbial consortium of Punjab Agricultural University (PAU decomposer), which is composed of Bacillus sp., Delftia sp., Pseudomonas sp., Lysinibacillus fusiform, Arthrobacter nicotianae, Paenibaccilus ehimensis, Aspergillus sp. and Trichoderma sp. Bio-digested slurry, the underutilized spent waste from cattle dung-based biogas plants was used as a growth medium for the mass multiplication of consortium. Soaked and chopped RS was pretreated with consortium and individual cultures in triplicate sets for 5 and 10 d and were analyzed for biochemical parameters along with cellulolytic activity. The pretreated straw was used for biogas production in 2 L capacity anaerobic digesters. Cellulolytic activities i.e., endoglucanase (0.071 IU/mL), exoglucanase (0.027 IU/mL) and β-glucosidase (3.734 IU/mL) of consortium treated RS were higher as compared to untreated RS. Biogas production from consortium pretreated RS was 187.45 L/kg RS which is 45.93% more as compared to untreated RS with 128.45 L/kg RS of biogas. This could be attributed to the synergistic cellulolytic activities of PAU decomposer leading to enhanced biogas production from RS.

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来源期刊
Energy nexus
Energy nexus Energy (General), Ecological Modelling, Renewable Energy, Sustainability and the Environment, Water Science and Technology, Agricultural and Biological Sciences (General)
CiteScore
7.70
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0.00%
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0
审稿时长
109 days
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