超高效液相色谱-质谱联用法测定食品中l -麦角硫因

IF 5.2 Q1 FOOD SCIENCE & TECHNOLOGY
Hongwen Zhai, Lei Sun, Sufang Fan, Jing Wang, Meirong Cao, Hongyu Ma, Qiang Li, Yan Zhang
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引用次数: 0

摘要

L-麦角硫酮(L-EGT)具有良好的抗氧化活性,已被应用于食品、制药和化妆品行业。本研究建立了一种新的高效、灵敏的超高效液相色谱-串联质谱法(UPLC-MS/MS)测定食品中L-EGT的方法。样品用甲醇-水(70:30,V/V)萃取,用亲水相互作用液相色谱法(HILIC)分离,用三重四极杆质谱法检测。对不同的产品进行了验证研究,定量限为20μg/kg(牛奶、无酒精饮料、乳制品)和40μg/kg(谷物棒、巧克力)。L-EGT定量在5–200 ng/mL范围内实现了良好的线性(相关系数(R2)≥0.999)。方法的回收率为83.7%−107.5%,相对标准偏差为0.88%−6.84%(n = 6) )符合测定食品中L-EGT所需的性能标准。最后,通过对实际样品的分析,验证了该方法的适用性。总的来说,所开发的方法简单、可靠、准确、稳定,可用于食品中L-EGT的常规分析。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Determination of L-ergothioneine in food by UPLC-MS/MS method

L-Ergothioneine (L-EGT) possesses excellent antioxidant activity and has been used in the food, pharmaceuticals and cosmetics industries. In this study, a new efficient and sensitive ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established for the quantitative determination of L-EGT in food. The sample was extracted with methanol-water (70:30, V/V), separated by hydrophilic interaction liquid chromatography (HILIC) and detected by triple-quadrupole mass spectrometry. Validation studies were carried out on different product and the limit of quantitation was 20 μg/kg (milk, alcohol-free beverages, dairy products) and 40 µg/kg (cereal bars, chocolate). Excellent linearity (correlation coefficient (R2) ≥ 0.999) was achieved for L-EGT quantification in the range of 5–200 ng/mL. The recoveries of the method (83.7%−107.5%) and the relative standard deviation (RSD, 0.88%−6.84% (n = 6)) meet the performance criteria required for the determination of L-EGT in food. Finally, the applicability of the method was tested by analysing actual samples. In general, the method developed is simple, reliable, accurate, and stable and could be useful for routine analyses of L-EGT in food.

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CiteScore
5.80
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