厚朴药孔治疗局部脂肪的作用机制及网络药理学与实验研究

IF 2.8 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE
Won Jun Choi , Mi Hye Kim , Nayoung Park, Jae Yoon Chung, Sang Jun Park, Woong Mo Yang
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引用次数: 0

摘要

背景近年来,由于各种原因,出现了对局限性脂肪进行无创治疗的需求。本研究证实厚朴(MO)是否通过促进脂解和抑制脂肪生成来减少局部脂肪。方法利用MO活性化合物相关基因构建网络,通过功能富集分析预测MO的作用方式。根据网络分析结果,在肥胖C57BL/6J小鼠的腹股沟脂肪垫中注射100µL 2 mg/mL MO药效针6周。结果MO网络可能会影响AMPK信号通路。MO药效针刺降低了HFD诱导的肥胖小鼠腹股沟脂肪的重量和大小。注射MO后,AMPK的磷酸化作用随脂肪酶的增加而显著增加。此外,MO注射抑制了脂肪酸合成相关介质的表达水平。结论MO药物能促进AMPK的表达,对激活脂解和抑制脂肪生成具有有益作用。MO的药物穿刺可以作为治疗局部脂肪组织的非手术替代疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effect and mechanism of Magnolia officinalis pharmacopuncture for treating localized fat via network pharmacology and experimental study

Background

Recently, for various reasons, the need for non-invasive treatment for localized fat has emerged. This study confirmed whether Magnolia officinalis (MO) pharmacopuncture reduces localized fat by promoting lipolysis and inhibiting adipogenesis.

Methods

The network was built using genes related to the active compound of MO and the mode of action of MO was predicted by the functional enrichment analysis. Based on the result from network analysis, 100 µL of 2 mg/mL MO pharmacopuncture was injected into the inguinal fat pad for 6 weeks in obese C57BL/6J mice. Normal saline was injected into the right-side inguinal fat pad as a self-control.

Results

It was expected that the AMP-activated protein kinase (AMPK) signaling pathway would be affected by the MO Network. MO pharmacopuncture reduced the weight and size of inguinal fat in HFD-induced obese mice. The phosphorylation of AMPK along with the increases of lipases was significantly increased by MO injection. Also, the expression levels of fatty acid synthesize-related mediators were suppressed by MO injection.

Conclusion

Our results demonstrated that MO pharmacopuncture promoted the expression of AMPK, which has beneficial effects on activation of lipolysis and inhibition of lipogenesis. Pharmacopuncture of MO can be a non-surgical alternative therapy in the treatment of local fat tissue.

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来源期刊
Integrative Medicine Research
Integrative Medicine Research Medicine-Complementary and Alternative Medicine
CiteScore
6.50
自引率
2.90%
发文量
65
审稿时长
12 weeks
期刊介绍: Integrative Medicine Research (IMR) is a quarterly, peer-reviewed journal focused on scientific research for integrative medicine including traditional medicine (emphasis on acupuncture and herbal medicine), complementary and alternative medicine, and systems medicine. The journal includes papers on basic research, clinical research, methodology, theory, computational analysis and modelling, topical reviews, medical history, education and policy based on physiology, pathology, diagnosis and the systems approach in the field of integrative medicine.
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