ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue
{"title":"北京市密云区人粪便大肠杆菌的基因分型特征及其与多药耐药性的关系","authors":"ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue","doi":"10.3967/bes2023.050","DOIUrl":null,"url":null,"abstract":"<div><h3>Objective</h3><p>To explore the genotyping characteristics of human fecal <em>Escherichia coli</em> (<em>E. coli</em>) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of <em>E. coli</em> in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.</p></div><div><h3>Methods</h3><p>Over a period of 3 years, 94 <em>E. coli</em> strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).</p></div><div><h3>Results</h3><p>This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).</p></div><div><h3>Conclusion</h3><p>We developed a random forest (RF) prediction model of <em>TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId</em> to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.</p></div>","PeriodicalId":55364,"journal":{"name":"Biomedical and Environmental Sciences","volume":"36 5","pages":"Pages 406-417"},"PeriodicalIF":3.0000,"publicationDate":"2023-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing\",\"authors\":\"ZHANG Wei Wei , ZHU Xiao Lin , DENG Le Le , HAN Ya Jun , LI Zhuo Wei , WANG Jin Long , CHEN Yong Liang , WANG Ao Lin , TIAN Er Li , CHENG Bin , XU Lin Hua , CHEN Yi Cong , TIAN Li Li , HE Guang Xue\",\"doi\":\"10.3967/bes2023.050\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objective</h3><p>To explore the genotyping characteristics of human fecal <em>Escherichia coli</em> (<em>E. coli</em>) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of <em>E. coli</em> in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.</p></div><div><h3>Methods</h3><p>Over a period of 3 years, 94 <em>E. coli</em> strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).</p></div><div><h3>Results</h3><p>This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).</p></div><div><h3>Conclusion</h3><p>We developed a random forest (RF) prediction model of <em>TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId</em> to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.</p></div>\",\"PeriodicalId\":55364,\"journal\":{\"name\":\"Biomedical and Environmental Sciences\",\"volume\":\"36 5\",\"pages\":\"Pages 406-417\"},\"PeriodicalIF\":3.0000,\"publicationDate\":\"2023-05-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical and Environmental Sciences\",\"FirstCategoryId\":\"1089\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0895398823000697\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical and Environmental Sciences","FirstCategoryId":"1089","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0895398823000697","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
Genotyping Characteristics of Human Fecal Escherichia coli and Their Association with Multidrug Resistance in Miyun District, Beijing
Objective
To explore the genotyping characteristics of human fecal Escherichia coli (E. coli) and the relationships between antibiotic resistance genes (ARGs) and multidrug resistance (MDR) of E. coli in Miyun District, Beijing, an area with high incidence of infectious diarrheal cases but no related data.
Methods
Over a period of 3 years, 94 E. coli strains were isolated from fecal samples collected from Miyun District Hospital, a surveillance hospital of the National Pathogen Identification Network. The antibiotic susceptibility of the isolates was determined by the broth microdilution method. ARGs, multilocus sequence typing (MLST), and polymorphism trees were analyzed using whole-genome sequencing data (WGS).
Results
This study revealed that 68.09% of the isolates had MDR, prevalent and distributed in different clades, with a relatively high rate and low pathogenicity. There was no difference in MDR between the diarrheal (49/70) and healthy groups (15/24).
Conclusion
We developed a random forest (RF) prediction model of TEM.1 + baeR + mphA + mphB + QnrS1 + AAC.3-IId to identify MDR status, highlighting its potential for early resistance identification. The causes of MDR are likely mobile units transmitting the ARGs. In the future, we will continue to strengthen the monitoring of ARGs and MDR, and increase the number of strains to further verify the accuracy of the MDR markers.
期刊介绍：
Biomedical and Environmental Sciences (BES) is a peer-reviewed journal jointly established by the Chinese Center for Disease Control and Prevention (China CDC) and the Coulston International Corporation (CIC), USA in 1988, and is published monthly by Elsevier. It is indexed by SCI, PubMed, and CA.
Topics covered by BES include infectious disease prevention, chronic and non-communicable disease prevention, disease control based on preventive medicine, and public health theories. It also focuses on the health impacts of environmental factors in people''s daily lives and work, including air quality, occupational hazards, and radiation hazards.
Article types considered for publication include original articles, letters to the editor, reviews, research highlights, and policy forum.
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