ZxABCG11在苜蓿中的过表达通过增加表皮蜡的沉积增强苜蓿对干旱和高温的耐受性

IF 6 1区 农林科学 Q1 AGRONOMY
Linbo Liu , Aike Bao , Hujun Li , Wanpeng Bai , Haishuang Liu , Ye Tian , Yiying Zhao , Fangchen Xia , Suomin Wang
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引用次数: 5

摘要

干旱和高温胁迫导致苜蓿的产量损失,苜蓿是世界各地种植的饲料作物。提高其耐旱性和耐热性是在炎热干旱地区维持苜蓿生产力所必需的。角质蜡在陆地植物的空中表面形成一道保护屏障,以抵御环境压力。ABCG11编码一种ATP结合盒(ABC)转运蛋白,在角质蜡转运途径中发挥作用。本研究采用根癌农杆菌介导的转化方法,将来自旱生植物Zygophyllum xanthoxylum的ZxABCG11导入苜蓿中。与野生型相比,转基因苜蓿在正常条件下生长更快,蜡晶密度更高,叶片角质层更厚。在温室条件下,无论是干旱还是高温处理,转基因苜蓿的株高和地上部生物量都显著高于WT。在炎热干旱的地区,转基因苜蓿在田间条件下表现出良好的生长和比WT高50%的干草产量。ZxABCG11的过表达上调了蜡质相关基因,导致更多的表皮蜡质沉积,这有助于降低角质层的通透性,从而提高转基因苜蓿的保水性和光合作用能力。因此,ZxABCG11的过表达可以通过增加表皮蜡的沉积来同时提高苜蓿的生物量产量、耐旱性和耐热性。我们的研究为开发适合在炎热、干旱、边缘地区种植的新型饲草品种提供了一条很有前途的途径。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Overexpression of ZxABCG11 from Zygophyllum xanthoxylum enhances tolerance to drought and heat in alfalfa by increasing cuticular wax deposition

Drought and heat stresses cause yield losses in alfalfa, a forage crop cultivated worldwide. Improving its drought and heat tolerance is desirable for maintaining alfalfa productivity in hot, arid regions. Cuticular wax forms a protective barrier on aerial surfaces of land plants against environmental stresses. ABCG11 encodes an ATP binding cassette (ABC) transporter that functions in the cuticular wax transport pathway. In this study, ZxABCG11 from the xerophyte Zygophyllum xanthoxylum was introduced into alfalfa by Agrobacterium tumefaciens-mediated transformation. Compared to the wild type (WT), transgenic alfalfa displayed faster growth, higher wax crystal density, and thicker cuticle on leaves under normal condition. Under either drought or heat treatment in greenhouse conditions, the plant height and shoot biomass of transgenic lines were significantly higher than those of the WT. Transgenic alfalfa showed excellent growth and 50% greater hay yield than WT under field conditions in a hot, arid region. Overexpression of ZxABCG11 up-regulated wax-related genes and resulted in more cuticular wax deposition, which contributed to reduction of cuticle permeability and thus increased water retention and photosynthesis capacity of transgenic alfalfa. Thus, overexpression of ZxABCG11 can simultaneously improve biomass yield, drought and heat tolerance in alfalfa by increasing cuticular wax deposition. Our study provides a promising avenue for developing novel forage cultivars suitable for planting in hot, arid, marginal lands.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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