GhHSP24.7介导线粒体蛋白乙酰化调节棉花气孔导度对非生物胁迫的响应

IF 6 1区 农林科学 Q1 AGRONOMY
Wei Ma , Jie Li , Fengjun Liu , Tianzhen Zhang , Xueying Guan
{"title":"GhHSP24.7介导线粒体蛋白乙酰化调节棉花气孔导度对非生物胁迫的响应","authors":"Wei Ma ,&nbsp;Jie Li ,&nbsp;Fengjun Liu ,&nbsp;Tianzhen Zhang ,&nbsp;Xueying Guan","doi":"10.1016/j.cj.2022.09.005","DOIUrl":null,"url":null,"abstract":"<div><p>During seed germination, the cotton chaperone protein HSP24.7 regulates the release, from the mitochondrial electron transport chain, of reactive oxygen species (ROS), a stimulative signal regulating germination. The function of HSP24.7 during vegetative stages remains largely unknown. Here we propose that suppression of <em>GhHSP24.7</em> in cotton seedlings increases tolerance to heat and drought stress. Elevation of <em>GhHSP24.7</em> was found to be positively associated with endogenous levels of ROS. We identified a new client protein of GhHSP24.7, cotton lysine deacetylase (GhHDA14), which is involved in mitochondrial protein modification. Elevated levels of <em>GhHSP24.7</em> suppressed deacetylase activity in mitochondria, leading to increased acetylation of mitochondrial proteins enriched in the subunit of F-type ATPase, V-type ATPase, and cytochrome <em>C</em> reductase, ultimately reducing leaf ATP content. Consequently, in combination with altered ROS content, <em>GhHSP24.7</em> transgenic lines were unable to coordinate stomatal closure under stress. The regulation circuit composed of GhHSP24.7 and GhHDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"GhHSP24.7 mediates mitochondrial protein acetylation to regulate stomatal conductance in response to abiotic stress in cotton\",\"authors\":\"Wei Ma ,&nbsp;Jie Li ,&nbsp;Fengjun Liu ,&nbsp;Tianzhen Zhang ,&nbsp;Xueying Guan\",\"doi\":\"10.1016/j.cj.2022.09.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>During seed germination, the cotton chaperone protein HSP24.7 regulates the release, from the mitochondrial electron transport chain, of reactive oxygen species (ROS), a stimulative signal regulating germination. The function of HSP24.7 during vegetative stages remains largely unknown. Here we propose that suppression of <em>GhHSP24.7</em> in cotton seedlings increases tolerance to heat and drought stress. Elevation of <em>GhHSP24.7</em> was found to be positively associated with endogenous levels of ROS. We identified a new client protein of GhHSP24.7, cotton lysine deacetylase (GhHDA14), which is involved in mitochondrial protein modification. Elevated levels of <em>GhHSP24.7</em> suppressed deacetylase activity in mitochondria, leading to increased acetylation of mitochondrial proteins enriched in the subunit of F-type ATPase, V-type ATPase, and cytochrome <em>C</em> reductase, ultimately reducing leaf ATP content. Consequently, in combination with altered ROS content, <em>GhHSP24.7</em> transgenic lines were unable to coordinate stomatal closure under stress. The regulation circuit composed of GhHSP24.7 and GhHDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514122002185\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514122002185","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 3

摘要

在种子发芽过程中,棉花伴侣蛋白HSP24.7调节线粒体电子传输链释放活性氧(ROS),这是一种调节发芽的刺激信号。HSP24.7在营养期的功能在很大程度上仍是未知的。在这里,我们提出抑制棉花幼苗中的GhHSP24.7可以提高对高温和干旱胁迫的耐受性。发现GhHSP24.7的升高与内源性ROS水平呈正相关。我们鉴定了GhHSP24.7的一种新的客户蛋白,棉花赖氨酸脱乙酰酶(GhHDA14),它参与线粒体蛋白修饰。GhHSP24.7水平的升高抑制了线粒体中的脱乙酰酶活性,导致富含F型ATP酶、V型ATP酶和细胞色素C还原酶亚基的线粒体蛋白的乙酰化增加,最终降低了叶片ATP含量。因此,与ROS含量的改变相结合,GhHSP24.7转基因系在胁迫下无法协调气孔关闭。由GhHSP24.7和GhHDA14组成的调节回路代表了植物非生物胁迫反应中的翻译后水平机制,该机制整合了ROS和ATP的调节。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
GhHSP24.7 mediates mitochondrial protein acetylation to regulate stomatal conductance in response to abiotic stress in cotton

During seed germination, the cotton chaperone protein HSP24.7 regulates the release, from the mitochondrial electron transport chain, of reactive oxygen species (ROS), a stimulative signal regulating germination. The function of HSP24.7 during vegetative stages remains largely unknown. Here we propose that suppression of GhHSP24.7 in cotton seedlings increases tolerance to heat and drought stress. Elevation of GhHSP24.7 was found to be positively associated with endogenous levels of ROS. We identified a new client protein of GhHSP24.7, cotton lysine deacetylase (GhHDA14), which is involved in mitochondrial protein modification. Elevated levels of GhHSP24.7 suppressed deacetylase activity in mitochondria, leading to increased acetylation of mitochondrial proteins enriched in the subunit of F-type ATPase, V-type ATPase, and cytochrome C reductase, ultimately reducing leaf ATP content. Consequently, in combination with altered ROS content, GhHSP24.7 transgenic lines were unable to coordinate stomatal closure under stress. The regulation circuit composed of GhHSP24.7 and GhHDA14 represents a post-translation level mechanism in plant abiotic stress responses that integrates the regulation of ROS and ATP.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信