麻疯树叶片的化学成分

IF 4.7 4区 医学 Q1 CHEMISTRY, MEDICINAL
Yingjie Wang , Di Zhou , Xiaolin Bai , Qingqi Meng , Haihui Xie , Guojiang Wu , Gang Chen , Yue Hou , Ning Li
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引用次数: 0

摘要

目的研究麻疯树叶片的化学成分,并评价其对脂多糖(LPS)激活的BV-2小胶质细胞的抑制作用。方法采用大孔吸附树脂、硅胶、ODS、柱色谱、半制备型高效液相色谱等方法,分离得到莪术叶正丁醇提取物。通过MS、NMR、ECD和其他光谱方法对化合物的结构进行了鉴定。此外,通过测量过度活化的BV-2细胞中一氧化氮(NO)的产生来评估分离的化合物的抗神经炎症作用。结果17个化合物,包括(7R,8S)-crataegifin A-4-O-β-D-吡喃葡糖苷(1)、9),(2S,3S)-表儿茶素(10),(2R,3S)—儿茶素(11),分离鉴定了异卵黄蛋白(12)、柚皮素-7-O-β-D-吡喃葡糖苷(13)、狼毒素(14)、新狼毒素B(15)、异狼毒素A(16)和绒毛素5-O-β-D-葡萄糖苷(17)。化合物2、4和8显著抑制LPS激活的BV-2小胶质细胞中NO的释放,IC50值分别为18.34、29.33和26.30μmol/L。结论化合物1为新化合物,化合物2、3、8、14–17为首次从麻风树属植物中分离得到。此外,木脂素显著抑制NO的释放,糖基化后抑制活性降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical constituents from leaves of Jatropha curcas

Objective

To investigate the chemical constituents from the leaves of Jatropha curcas and evaluate their inhibition on lipopolysaccharide (LPS)-activated BV-2 microglia cells.

Methods

The n-BuOH extract of the leaves of J. curcas was isolated by macroporous adsorption resin, silica gel, ODS, column chromatography and semi-preparative HPLC. The structures of the compounds were identified by MS, NMR, ECD, and other spectroscopic methods. In addition, anti-neuroinflammatory effects of isolated compounds were evaluated by measuring the production of nitric oxide (NO) in over-activated BV-2 cells.

Results

Seventeen compounds, including (7R,8S)-crataegifin A-4-O-β-D-glucopyranoside (1), (8R,8′R)-arctigenin (2), arctigenin-4′-O-β-D-glucopyranoside (3), (-)-syringaresinol (4), syringaresinol-4′-O-β-D-glucopyranoside (5), (-)-pinoresinol (6), pinoresinol-4′-O-β-D-glucopyranoside (7), buddlenol D (8), (2R,3R)-dihydroquercetin (9), (2S,3S)-epicatechin (10), (2R,3S)-catechin (11), isovitexin (12), naringenin-7-O-β-D-glucopyranoside (13), chamaejasmin (14), neochamaejasmin B (15), isoneochamaejasmin A (16), and tomentin-5-O-β-D-glucopyranoside (17) were isolated and identified. Compounds 2, 4 and 8 significantly inhibited the release of NO in BV-2 microglia activated by LPS, with IC50 values of 18.34, 29.33 and 26.30 μmol/L, respectively.

Conclusion

Compound 1 is a novel compound, and compounds 2, 3, 8, 1417 are isolated from Jatropha genus for the first time. In addition, the lignans significantly inhibited NO release and the inhibitory activity was decreased after glycosylation.

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来源期刊
Chinese Herbal Medicines
Chinese Herbal Medicines CHEMISTRY, MEDICINAL-
CiteScore
4.40
自引率
5.30%
发文量
629
审稿时长
10 weeks
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