Henna Lappi , Maija Kauppila , Katriina Aalto-Setälä , Anni Mörö
{"title":"3D生物打印的人类诱导多能干细胞衍生心脏模型:用于疾病建模和药物筛选的功能性和患者衍生体外模型","authors":"Henna Lappi , Maija Kauppila , Katriina Aalto-Setälä , Anni Mörö","doi":"10.1016/j.bprint.2023.e00313","DOIUrl":null,"url":null,"abstract":"<div><p>More relevant human tissue models are needed to produce reliable results when studying disease mechanisms of genetic diseases and developing or testing novel drugs in cardiac tissue engineering (TE). Three-dimensional (3D) bioprinting enables physiologically relevant positioning of the cells inside the growth matrix according to the detailed digital design. Here we combined human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) with methacrylated gelatin (GelMA) and collagen I-based bioink and 3D extrusion bioprinted a cardiac <em>in vitro</em> model for disease modeling and drug screening. Bioprinted constructs were characterized for their rheological properties, swelling behavior, degradation, as well as shape fidelity. The printed structures demonstrated good mechanical properties and high shape fidelity upon culture. Immunocytochemistry revealed elongated hiPSC-CMs growing inside the structures and the presence of the connexin 43 marker, indicating cardiac gap junctions between printed cells and tissue formation. Extensive functional analyses with calcium imaging showed normal functionality and calcium-handling properties for hiPSC-CMs. Finally, suitability of this 3D bioprinted construct for patient-specific disease modeling was demonstrated by bioprinting hiPSC-CMs from a patient carrying an inherited gene mutation causing catecholaminergic polymorphic ventricular tachycardia (CPVT). CPVT hiPSC-CMs responded to adrenaline treatment in the 3D bioprinted model in a manner that is characteristic for CPVT disease specific phenotype. Thus, the 3D bioprinted hiPSC-CM <em>in vitro</em> model has great potential for disease modeling and drug screening in cardiac tissue engineering.</p></div>","PeriodicalId":37770,"journal":{"name":"Bioprinting","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The 3D bioprinted human induced pluripotent stem cell-derived cardiac model: Toward functional and patient-derived in vitro models for disease modeling and drug screening\",\"authors\":\"Henna Lappi , Maija Kauppila , Katriina Aalto-Setälä , Anni Mörö\",\"doi\":\"10.1016/j.bprint.2023.e00313\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>More relevant human tissue models are needed to produce reliable results when studying disease mechanisms of genetic diseases and developing or testing novel drugs in cardiac tissue engineering (TE). Three-dimensional (3D) bioprinting enables physiologically relevant positioning of the cells inside the growth matrix according to the detailed digital design. Here we combined human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) with methacrylated gelatin (GelMA) and collagen I-based bioink and 3D extrusion bioprinted a cardiac <em>in vitro</em> model for disease modeling and drug screening. Bioprinted constructs were characterized for their rheological properties, swelling behavior, degradation, as well as shape fidelity. The printed structures demonstrated good mechanical properties and high shape fidelity upon culture. Immunocytochemistry revealed elongated hiPSC-CMs growing inside the structures and the presence of the connexin 43 marker, indicating cardiac gap junctions between printed cells and tissue formation. Extensive functional analyses with calcium imaging showed normal functionality and calcium-handling properties for hiPSC-CMs. Finally, suitability of this 3D bioprinted construct for patient-specific disease modeling was demonstrated by bioprinting hiPSC-CMs from a patient carrying an inherited gene mutation causing catecholaminergic polymorphic ventricular tachycardia (CPVT). CPVT hiPSC-CMs responded to adrenaline treatment in the 3D bioprinted model in a manner that is characteristic for CPVT disease specific phenotype. Thus, the 3D bioprinted hiPSC-CM <em>in vitro</em> model has great potential for disease modeling and drug screening in cardiac tissue engineering.</p></div>\",\"PeriodicalId\":37770,\"journal\":{\"name\":\"Bioprinting\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-10-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioprinting\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2405886623000568\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"Computer Science\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioprinting","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2405886623000568","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Computer Science","Score":null,"Total":0}
The 3D bioprinted human induced pluripotent stem cell-derived cardiac model: Toward functional and patient-derived in vitro models for disease modeling and drug screening
More relevant human tissue models are needed to produce reliable results when studying disease mechanisms of genetic diseases and developing or testing novel drugs in cardiac tissue engineering (TE). Three-dimensional (3D) bioprinting enables physiologically relevant positioning of the cells inside the growth matrix according to the detailed digital design. Here we combined human induced pluripotent stem cell (hiPSC)-derived cardiomyocytes (CMs) with methacrylated gelatin (GelMA) and collagen I-based bioink and 3D extrusion bioprinted a cardiac in vitro model for disease modeling and drug screening. Bioprinted constructs were characterized for their rheological properties, swelling behavior, degradation, as well as shape fidelity. The printed structures demonstrated good mechanical properties and high shape fidelity upon culture. Immunocytochemistry revealed elongated hiPSC-CMs growing inside the structures and the presence of the connexin 43 marker, indicating cardiac gap junctions between printed cells and tissue formation. Extensive functional analyses with calcium imaging showed normal functionality and calcium-handling properties for hiPSC-CMs. Finally, suitability of this 3D bioprinted construct for patient-specific disease modeling was demonstrated by bioprinting hiPSC-CMs from a patient carrying an inherited gene mutation causing catecholaminergic polymorphic ventricular tachycardia (CPVT). CPVT hiPSC-CMs responded to adrenaline treatment in the 3D bioprinted model in a manner that is characteristic for CPVT disease specific phenotype. Thus, the 3D bioprinted hiPSC-CM in vitro model has great potential for disease modeling and drug screening in cardiac tissue engineering.
期刊介绍:
Bioprinting is a broad-spectrum, multidisciplinary journal that covers all aspects of 3D fabrication technology involving biological tissues, organs and cells for medical and biotechnology applications. Topics covered include nanomaterials, biomaterials, scaffolds, 3D printing technology, imaging and CAD/CAM software and hardware, post-printing bioreactor maturation, cell and biological factor patterning, biofabrication, tissue engineering and other applications of 3D bioprinting technology. Bioprinting publishes research reports describing novel results with high clinical significance in all areas of 3D bioprinting research. Bioprinting issues contain a wide variety of review and analysis articles covering topics relevant to 3D bioprinting ranging from basic biological, material and technical advances to pre-clinical and clinical applications of 3D bioprinting.