Thi Phuong Thao Truong, Thanh Men Tran, Thi Xuan Trang Dai, Chi Linh Tran
{"title":"褐藻海洋对苯二酚抗高血糖和2型糖尿病活性研究","authors":"Thi Phuong Thao Truong, Thanh Men Tran, Thi Xuan Trang Dai, Chi Linh Tran","doi":"10.1016/j.jtcme.2023.03.007","DOIUrl":null,"url":null,"abstract":"<div><h3>Background and aims</h3><p>Brown algae (<em>Dictyopteris polypodioides</em>) extract (DP) presented high inhibitory potential against α-amylase. The present study aims to isolate, purify and evaluate the antihyperglycemic and anti-type 2 diabetic activities of marine hydroquinone from DP.</p></div><div><h3>Methods</h3><p>Marine hydroquinones were isolated using silica gel, HPLC, and NMR spectroscopy was used to identify compound 1 and compound 2 as zonarol and isozonarol, respectively. The anti-hyperglycemic and anti-type 2 diabetic activities of zonarol were investigated by <em>in vitro</em> assay (α-amylase, α-glucosidase), Lineweaver–Burk plot and Type 2 diabetes mellitus model (T2DM) mice induced by streptozotocin (STZ).</p></div><div><h3>Result</h3><p>Zonarol had the highest content and the strongest inhibitory activity against α-glucosidase (IC<sub>50</sub> value of 6.03 mg L<sup>−1</sup>) and α-amylase (IC<sub>50</sub> value of 19.29 mg L<sup>−1</sup>) in a competitive inhibition and mix-type manner, respectively. The maltose and starch loading tests revealed that zonarol significantly reduced postprandial glycemia after 30 min loading (9.12 and 8.12 mg/dL, respectively), compared to normal (11.37 and 12.37 mg/dL, respectively). Zonarol exhibited pancreatic islet cell rejuvenation, as evidenced by increased pancreatic islet mass, and hence helps in the restoration of insulin levels and therefore improves the glucose metabolism in STZ-induced diabetic mice. Zonarol treatment in T2DM elevated abundant levels of main SCFAs (propionate, butyrate, and valeric acid), which are closely related to glucose metabolism homeostasis.</p></div><div><h3>Conclusion</h3><p>Our finding indicates that zonarol could be used as a food supplement to treat hyperglycemia and diabetes.</p></div>","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":null,"pages":null},"PeriodicalIF":4.3000,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Antihyperglycemic and anti-type 2 diabetic activity of marine hydroquinone isolated from brown algae (Dictyopteris polypodioides)\",\"authors\":\"Thi Phuong Thao Truong, Thanh Men Tran, Thi Xuan Trang Dai, Chi Linh Tran\",\"doi\":\"10.1016/j.jtcme.2023.03.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Background and aims</h3><p>Brown algae (<em>Dictyopteris polypodioides</em>) extract (DP) presented high inhibitory potential against α-amylase. The present study aims to isolate, purify and evaluate the antihyperglycemic and anti-type 2 diabetic activities of marine hydroquinone from DP.</p></div><div><h3>Methods</h3><p>Marine hydroquinones were isolated using silica gel, HPLC, and NMR spectroscopy was used to identify compound 1 and compound 2 as zonarol and isozonarol, respectively. The anti-hyperglycemic and anti-type 2 diabetic activities of zonarol were investigated by <em>in vitro</em> assay (α-amylase, α-glucosidase), Lineweaver–Burk plot and Type 2 diabetes mellitus model (T2DM) mice induced by streptozotocin (STZ).</p></div><div><h3>Result</h3><p>Zonarol had the highest content and the strongest inhibitory activity against α-glucosidase (IC<sub>50</sub> value of 6.03 mg L<sup>−1</sup>) and α-amylase (IC<sub>50</sub> value of 19.29 mg L<sup>−1</sup>) in a competitive inhibition and mix-type manner, respectively. The maltose and starch loading tests revealed that zonarol significantly reduced postprandial glycemia after 30 min loading (9.12 and 8.12 mg/dL, respectively), compared to normal (11.37 and 12.37 mg/dL, respectively). Zonarol exhibited pancreatic islet cell rejuvenation, as evidenced by increased pancreatic islet mass, and hence helps in the restoration of insulin levels and therefore improves the glucose metabolism in STZ-induced diabetic mice. Zonarol treatment in T2DM elevated abundant levels of main SCFAs (propionate, butyrate, and valeric acid), which are closely related to glucose metabolism homeostasis.</p></div><div><h3>Conclusion</h3><p>Our finding indicates that zonarol could be used as a food supplement to treat hyperglycemia and diabetes.</p></div>\",\"PeriodicalId\":3,\"journal\":{\"name\":\"ACS Applied Electronic Materials\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":4.3000,\"publicationDate\":\"2023-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"ACS Applied Electronic Materials\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2225411023000421\",\"RegionNum\":3,\"RegionCategory\":\"材料科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"ENGINEERING, ELECTRICAL & ELECTRONIC\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2225411023000421","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
Antihyperglycemic and anti-type 2 diabetic activity of marine hydroquinone isolated from brown algae (Dictyopteris polypodioides)
Background and aims
Brown algae (Dictyopteris polypodioides) extract (DP) presented high inhibitory potential against α-amylase. The present study aims to isolate, purify and evaluate the antihyperglycemic and anti-type 2 diabetic activities of marine hydroquinone from DP.
Methods
Marine hydroquinones were isolated using silica gel, HPLC, and NMR spectroscopy was used to identify compound 1 and compound 2 as zonarol and isozonarol, respectively. The anti-hyperglycemic and anti-type 2 diabetic activities of zonarol were investigated by in vitro assay (α-amylase, α-glucosidase), Lineweaver–Burk plot and Type 2 diabetes mellitus model (T2DM) mice induced by streptozotocin (STZ).
Result
Zonarol had the highest content and the strongest inhibitory activity against α-glucosidase (IC50 value of 6.03 mg L−1) and α-amylase (IC50 value of 19.29 mg L−1) in a competitive inhibition and mix-type manner, respectively. The maltose and starch loading tests revealed that zonarol significantly reduced postprandial glycemia after 30 min loading (9.12 and 8.12 mg/dL, respectively), compared to normal (11.37 and 12.37 mg/dL, respectively). Zonarol exhibited pancreatic islet cell rejuvenation, as evidenced by increased pancreatic islet mass, and hence helps in the restoration of insulin levels and therefore improves the glucose metabolism in STZ-induced diabetic mice. Zonarol treatment in T2DM elevated abundant levels of main SCFAs (propionate, butyrate, and valeric acid), which are closely related to glucose metabolism homeostasis.
Conclusion
Our finding indicates that zonarol could be used as a food supplement to treat hyperglycemia and diabetes.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
