表没食子儿茶素-3-没食子酸酯与纳米羟基磷灰石表面的化学键合增强其抗骨肉瘤的生物活性

Q1 Engineering
Jian Ren , Lingli Sun , Cairong Xiao , Shuoshuo Zhou , Qingyou Liang , Shili Sun , Chunlin Deng
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引用次数: 0

摘要

术后缺损修复结合清除残余癌症细胞仍然是恶性骨肿瘤治疗的主要临床挑战。表没食子儿茶素没食子酸酯(EGCG)是从绿茶中提取的一种天然产物,具有广泛的生物活性。在本研究中,我们研究了天然化合物EGCG与羟基磷灰石(HA)联合用于骨肉瘤术后治疗的抗骨肉瘤和成骨潜力。我们采用模板法结合表面修饰技术合成了分散良好的表面氨基功能化羟基磷灰石纳米粒子。然后,我们通过酰胺键将EGCG和HA纳米颗粒偶联,以防止生物分子的突然释放并提高其稳定性。结果表明,所制备的HA-EGCG纳米粒子具有与纯EGCG相同的抗氧化活性。HA-EGCG纳米颗粒在酸性肿瘤环境中通过酶相互作用表现出有效的EGCG释放,促进EGCG在肿瘤组织中的积累并提高其生物利用度。与纯EGCG和HA相比,HA-EGCG在体内外均表现出增强的抗癌活性。HA-EGCG能有效促进成骨分化。这种共价策略提供了一种简单的方法来制造pH和酶介导的递送平台,以提高EGCG的稳定性和生物利用度。本研究为设计与EGCG相结合的生物材料在骨病中的潜在应用提供了一种策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Chemical bonding of Epigallocatechin-3-gallate to the surface of nano-hydroxyapatite to enhance its biological activity for anti-osteosarcoma

Post-surgical defect repair combined with the elimination of residual cancer cells remains a major clinical challenge for the therapy of malignant bone tumors. As a natural product extracted from green tea, epigallocatechin-3-gallate (EGCG) has a wide range of biological activities. In this study, we investigated the anti-osteosarcoma and osteogenic potential of the natural compound EGCG in combination with hydroxyapatite (HA) for the post-operative treatment of osteosarcoma. We have synthesized well-dispersed surface amino-functionalized hydroxyapatite nanoparticles by the template method combined with surface modification techniques. Then, we conjugated EGCG with HA nanoparticles via amido linkage to prevent burst release of the biomolecules and improve their stability. The results showed that the as-prepared HA-EGCG nanoparticles had the same antioxidant activity as pure EGCG. The HA-EGCG nanoparticles demonstrated efficient EGCG release upon enzyme interactions in an acidic tumor environment, facilitating the accumulation of EGCG in tumor tissues and improving its bioavailability. Compared with pure EGCG and HA, HA-EGCG exhibited enhanced anticancer activity in vitro and in vivo. Furthermore, HA-EGCG could effectively promote osteogenic differentiation. This covalent strategy provides a simple method to fabricate a pH and enzyme-mediated delivery platform to refine the stability and bioavailability of EGCG. This research provides a strategy into designing biomaterials combined with EGCG for the potential application in bone diseases.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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