三维介质电泳微流控芯片分离富集循环肿瘤细胞的研究

IF 2.6 4区 工程技术 Q2 ENGINEERING, ELECTRICAL & ELECTRONIC
Linxia Jiang , Feng Liang , Mingxuan Huo, Meiqi Ju, Jiajun Xu, Shaowei Ju, Lihong Jin, Bingjun Shen
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引用次数: 2

摘要

循环肿瘤细胞(CTCs)是指肿瘤细胞流入人体外周血,是早期准确检测恶性肿瘤的重要指标之一。目的基于介电泳原理,进行了微流控微阵列分离富集CTC的设计和性能研究,利用介电泳力的差异和CTC与白细胞的运动轨迹,实现了CTC的分离富集。结果基于COMSOL软件仿真工具的芯片结构设计与优化,通过人乳腺癌症细胞MCF-7与白细胞的分离实验,选择60kHz作为细胞分离实验的频率。以人癌症细胞SMMC-7721和人白细胞混合物为研究对象,进行了CTC芯片分离富集实验。在5V电压和60kHz频率下,微流体芯片对人肝癌细胞SMMC-7721的三维介电电泳最大分离通量达到1.1mL/h,分离效率约为94%。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Study on three-dimensional dielectrophoresis microfluidic chip for separation and enrichment of circulating tumor cells

Study on three-dimensional dielectrophoresis microfluidic chip for separation and enrichment of circulating tumor cells

Background

Circulating tumor cells (CTCs) which means tumor cells shed into the peripheral blood of humans, are one of the important indicators in the early and accurate detection of malignancy.

Objective

This paper is based on the principle of dielectrophoresis, the design and performance research of microfluidic microarray separating and enriching CTCs was carried out, and the separation and enriching of CTCs was realized by ultilizing the difference in dielectrophoretic force and the movement trajectories between CTCs and leukocytes.

Results

Chip structure design and optimization based on the COMSOL software simulation tool, through the separation experiment of human breast cancer cell MCF-7 and white blood cells, 60 kHz was selected as the frequency of cell separation experiment. Taking human liver cancer cell SMMC-7721 and human leukocyte mixture as the research object, the chip separation and enrichment experiment of CTCs was conducted. At 5 V voltage and 60 kHz frequency, the maximum separation flux of three-dimensional dielectrophores is microfluidic chip for human liver cancer cell SMMC-7721 reached 1.1 mL/h, and the separation efficiency was about 94%.

Conclusion

The results of this research have important implications for separating, sorting and detecting CTCs.

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来源期刊
Microelectronic Engineering
Microelectronic Engineering 工程技术-工程:电子与电气
CiteScore
5.30
自引率
4.30%
发文量
131
审稿时长
29 days
期刊介绍: Microelectronic Engineering is the premier nanoprocessing, and nanotechnology journal focusing on fabrication of electronic, photonic, bioelectronic, electromechanic and fluidic devices and systems, and their applications in the broad areas of electronics, photonics, energy, life sciences, and environment. It covers also the expanding interdisciplinary field of "more than Moore" and "beyond Moore" integrated nanoelectronics / photonics and micro-/nano-/bio-systems. Through its unique mixture of peer-reviewed articles, reviews, accelerated publications, short and Technical notes, and the latest research news on key developments, Microelectronic Engineering provides comprehensive coverage of this exciting, interdisciplinary and dynamic new field for researchers in academia and professionals in industry.
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