负载miR-29a@ZIF-8纳米颗粒的工程多功能丝素/明胶水凝胶导管用于周围神经再生

Q1 Engineering
Hao Wang , Hongxia Wan , Qiqi Wang , Ying Ma , Guorui Su , Xiaodong Cao , Huichang Gao
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引用次数: 2

摘要

周围神经损伤(PNI)是一种常见的外科疾病。近年来,随着组织工程材料的发展,神经引导管有望取代自体神经移植,成为治疗PNI的新方法。在本工作中,我们采用扩散驱动交联方法开发了一种尺寸可灵活调节的多功能丝素蛋白(SF)/明胶-酪胺(GT)复合水凝胶导管。此外,负载miR-29a的ZIF-8纳米颗粒(miR-29a@ZIF-8)构建了递送系统并将其复合到SF/GT水凝胶导管中,以通过持续释放miR-29a来增强其生物活性和神经修复效果。体外细胞实验表明,SF/GT水凝胶导管可显著促进许旺细胞(SC)的髓鞘形成、PC12的神经元分化和轴突延伸​细胞。此外,值得一提的是,SF/GT水凝胶导管还可以通过促进巨噬细胞从M1表型向M2表型的转化来调节神经再生的免疫微环境,表明其作为神经引导导管在外周神经修复中的潜在应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Engineered multifunctional silk fibroin/gelatin hydrogel conduit loaded with miR-29a@ZIF-8 nanoparticles for peripheral nerve regeneration

Peripheral nerve injury (PNI) is a common surgical disease. In recent years, with the development of tissue engineering materials, nerve guidance conduit (NGC) is expected to replace autologous nerve transplantation and become a new method for the treatment of PNI. In this work, we developed a multifunctional silk fibroin (SF)/gelatin-tyramine (GT) composite hydrogel conduit with flexible adjustable size by using a diffusion-driven cross-linking method. Furthermore, the ZIF-8 nanoparticles loaded with miR-29a (miR-29a@ZIF-8) delivery system was constructed and compounded into SF/GT hydrogel conduit to enhance its bioactivity and neural repair effects through sustained miR-29a release. In vitro cell experiments showed that SF/GT hydrogel conduit could significantly promote the myelination of Schwann cells (SCs), neuronal differentiation and axon extension of PC12 ​cells. In addition, it was worth mentioning that SF/GT hydrogel conduit could also regulate the immune microenvironment of nerve regeneration by promoting the transformation of macrophages from M1 phenotype to M2 phenotype, indicating a potential application as nerve guidance conduit in peripheral nerve repair.

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来源期刊
Smart Materials in Medicine
Smart Materials in Medicine Engineering-Biomedical Engineering
CiteScore
14.00
自引率
0.00%
发文量
41
审稿时长
48 days
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