anti-E。母体初乳大肠杆菌抗体对新生奶牛肠道大肠杆菌定植及临床腹泻发展的影响

V. Gomes , B.P. Barros , D.I. Castro-Tardón , C.C. Martin , F.C.R. Santos , T. Knöbl , B.P. Santarosa , L.M. Padilha , D.J. Hurley
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引用次数: 0

摘要

大肠杆菌(E.coli)是粘膜组织定植的早期生物,对粘膜和全身炎症反应的发展有影响。为了了解免疫球蛋白G(IgG)、大肠杆菌特异性抗体和一般免疫因子转移到小牛体内对肠道微生物群的影响,在新生荷斯坦小牛身上进行了一项观察性研究,该研究跟踪了大肠杆菌在肠道的时间定殖,与IgG和识别大肠杆菌的抗体的总量相关。小牛接受了初乳管理的“金标准”,包括在分娩前接种牛初乳疫苗。本研究使用了20只荷斯坦母鼠,在健康雌性后代的简单分娩后产生了足够数量的优质初乳。每天对每头小牛进行腹泻监测和评分。在第(D)D1天(在摄入初乳之前),然后在饲喂初乳之后的D3、D7、D14和D28,从小牛身上采集血液和粪便样本。分别使用常规聚合酶链式反应和实时聚合酶链式反应定量评估从小牛粪便样本中分离的大肠杆菌的毒力基因和每克粪便的数量。采用酶联免疫吸附法测定血浆IgG总量和大肠杆菌结合抗体滴度。在D12观察到小牛腹泻的高峰。在摄入初乳之前未检测到IgG或大肠杆菌结合抗体(D1)。总IgG和结合抗体均显著增加D3。总IgG和结合IgG随小牛年龄的增长而下降。在D1和D14之间,粪便中的细菌总数(16S rRNA)相似。从D14到D28期间,细菌总数增加。D1的大肠杆菌数量最少。大肠杆菌的数量在D3-D7期间增加。每克粪便中大肠杆菌的数量在D14和D28之间下降。从小牛身上分离的大肠杆菌中经常发现稳定的毒素基因。此外,在本研究中未鉴定大肠杆菌K99。虽然肠道在生命早期就被大肠杆菌定殖,这与IgG和特异性抗体的峰值水平相对应,但随着被动免疫的减弱,大肠杆菌的数量也在减少。这表明,被动抗体的功能可能是控制定植和种子先天免疫功能,从而稳定小牛消化道中大肠杆菌的水平。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The role of anti-E. coli antibody from maternal colostrum on the colonization of newborn dairy calves gut with Escherichia coli and the development of clinical diarrhea

Escherichia coli (E. coli) is an early organism in the colonization of mucosal tissue and has an influence on the development of mucosal and systemic inflammatory responses. To understand the impact of transfer of Immunoglobulin G (IgG), E. coli-specific antibody and general immune factors to the calf on the gut microbiota, an observational study that followed the temporal colonization of the intestine with E. coli as correlated with the total quantity of IgG and the antibody recognizing E. coli, was conducted in newborn Holstein calves. The calves were subjected to the “gold standard” of colostrum management, including dam vaccination prior to delivery to prime colostrum. Twenty Holstein dams, producing an adequate quantity of good-quality colostrum following uncomplicated deliveries of healthy female offspring, were utilized in this study. Each calf was monitored and scored daily for diarrhea. Blood and fecal samples were collected from calves on day (D) D1 (before colostrum intake), then D3, D7, D14 and D28 after colostrum feeding. E. coli isolated from fecal samples of the calves were assessed for virulence genes and the quantity per gram of feces using conventional Polymerase chain reaction and Real-time polymerase chain reaction quantitative, respectively. The total quantity of plasma IgG and the titer of E. coli-binding antibody were measured by enzyme-linked immunosorbent assay. The peak in diarrhea in the calves was observed on D12. No IgG or E. coli-binding antibody were detected prior to colostrum intake (D1). Both total IgG and binding antibody sharply increased by D3. Total and binding IgG declined with calf age. The total number of bacteria (16S rRNA) in feces was similar between D1 and D14. The number of total bacteria increased over the period from D14 to D28. The number of E. coli was minimal on D1. The number of E. coli increased during the period D3-D7. A decrease in the number of E. coli per gram of feces declines between D14and D28. The stable toxin gene was frequently identified in the E. coli isolated from the calves. Further, E. coli K99 was not identified in this study. While the gut was colonized by E. coli early in life corresponding to the peak level of IgG and specific antibody, a decreasing number of E. coli were observed as the passive immunity waned. This suggests that the function of the passive antibody may be to control colonization and seed innate immune function that then stabilizes the level of E. coli in the digestive tract of calves.

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