Ningyi Xu , Xinyue Ma , Yue Cao , Huan Wang , Han Wu , Hongru Zheng , Chuanyu Yang , Ruiqi Zou , Jiayi Liu , Chunyan Sun , Yuan Yuan
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引用次数: 2
摘要
利用Au纳米粒子（AuNPs）对丙烯酰胺（AAm）的吸附作用和SYBR Green I（SGI）对双链DNA（dsDNA）的荧光特性，开发了一种用于丙烯酰胺（AAm）检测的荧光适体传感器。与适体与AAm的结合相比，适体与cDNA的更高亲和力可能有助于从适体/AAm复合物向适体/cDNA dsDNA的结构转换。将游离的适体吸附在AuNPs上并通过离心分离。随后，SGI被引入作为用于定量检测的荧光报告子。与传统的基于AuNPs的比色检测相比，该方法在0.005–50 mg/L范围内的灵敏度提高了3.18倍，检测下限为4.68μg/L。该方法已成功应用于油炸食品中。值得注意的是，这是一种低成本、通用的方法，为该领域快速筛查技术的发展提供了指导。
A novel fluorescent structure-switching aptasensor for the sensitive detection of acrylamide based on AuNPs-assisted separation of ssDNA
Fluorescent aptasensor was developed for acrylamide (AAm) detection by utilizing the adsorption effect of Au nanoparticles (AuNPs) and fluorescence properties of SYBR Green I (SGI) towards double-stranded DNA (dsDNA). Compared to the binding of aptamer with AAm, the higher affinity of aptamer with cDNA may facilitate a structure switching from the aptamer/AAm complex to aptamer/cDNA dsDNA. The free aptamers were adsorbed onto AuNPs and separated by centrifugation. Subsequently, SGI was introduced as a fluorescent reporter for quantitative detection. Compared to conventional AuNPs-based colorimetric detection, the sensitivity of this strategy was improved by 3.18-fold in the range of 0.005–50 mg/L with a low detection limit of 4.68 μg/L. The method has been successfully applied to analyze fried twists and biscuits. Notably, it is a low-cost and general method that provides guidance for the development of rapid screening technology in the field.
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