具有聚集诱导发射特征的ph响应纳米平台用于动脉粥样硬化中的脂滴成像

IF 13.3 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2023-10-20 DOI:10.1016/j.cej.2023.146792

Di Ma, Weihua Zhuang, Qi Liu, Jingruo Chen, Chengming Li, Shufen Li, Mao Chen

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引用次数: 0

摘要

疏水脂滴(LDs)特异性荧光探针在生物成像中显示出巨大的潜力。然而，它们的体内生物应用也受到其强疏水性的限制。为了解决这一问题，我们制备了一种新型的四苯基乙烯(TPE)荧光探针，即MeOTTI，以及ph响应聚合物胶束，以建立动脉粥样硬化中ld成像的纳米诊断系统。MeOTTI具有良好的lds特异性成像能力，抗光漂白能力较好，但水溶性较差。MeOTTI包封纳米颗粒(MeOTTI- pmea NPs)具有较好的水溶性和生物相容性。MeOTTI- pmea NPs在pH 7.4时表现出良好的稳定性，同时表现出良好的酸响应能力，加速了MeOTTI的释放。重要的是，MeOTTI-PMEA NPs可以有效地照亮细胞和小鼠动脉粥样硬化斑块中的ld。lds特异性探针负载纳米诊断系统的构建有望为生物成像新工具的开发创造热情。

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A pH-responsive nanoplatform with aggregation-induced emission features for lipid droplet imaging in atherosclerosis

Hydrophobic lipid droplets (LDs)-specific fluorescent probes have shown great potential in bioimaging. However, their in vivo bioapplications are also limited by their strong hydrophobic nature. To address this issue, we prepared a novel tetraphenylethylene (TPE)-based fluorescent probe, namely, MeOTTI, and a pH-responsive polymeric micelle to establish a nanodiagnostic system for the imaging of LDs in atherosclerosis. MeOTTI demonstrated good LDs-specific imaging capability with good anti-photobleaching ability but poor water solubility. MeOTTI-encapsulated nanoparticles (MeOTTI-PMEA NPs) have shown improved water solubility and biocompatibility of MeOTTI. MeOTTI-PMEA NPs exhibited great stability at pH 7.4, while they showed good acid-responsive ability with accelerated MeOTTI release. Importantly, MeOTTI-PMEA NPs could efficiently light up the LDs in cells and in mice atherosclerosis plaques. The construction of LDs-specific probe-loaded nanodiagnostic system is expected to create enthusiasm for the development of new tools for bioimaging.

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.