Erica L. Benard, Ismail Küçükaylak, Julia Hatzold, Kilian U. W. Berendes, Thomas J. Carney, Filippo Beleggia, Matthias Hammerschmidt
{"title":"wnt10a是斑马鱼中鳍折叠维持和成年非成对鳍变态所必需的。","authors":"Erica L. Benard, Ismail Küçükaylak, Julia Hatzold, Kilian U. W. Berendes, Thomas J. Carney, Filippo Beleggia, Matthias Hammerschmidt","doi":"10.1002/dvdy.672","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Mutations of human <i>WNT10A</i> are associated with odonto-ectodermal dysplasia syndromes. Here, we present analyses of <i>wnt10a</i> loss-of-function mutants in the zebrafish.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p><i>wnt10a</i> mutant zebrafish embryos display impaired tooth development and a collapsing median fin fold (MFF). Rescue experiments show that <i>wnt10a</i> is essential for MFF maintenance both during embryogenesis and later metamorphosis. The MFF collapse could not be attributed to increased cell death or altered proliferation rates of MFF cell types. Rather, <i>wnt10a</i> mutants show reduced expression levels of <i>dlx2a</i> in distal-most MFF cells, followed by compromised expression of <i>col1a1a</i> and other extracellular matrix proteins encoding genes. Transmission electron microscopy analysis shows that although dermal MFF compartments of <i>wnt10a</i> mutants initially are of normal morphology, with regular collagenous actinotrichia, positioning of actinotrichia within the cleft of distal MFF cells becomes compromised, coinciding with actinotrichia shrinkage and MFF collapse.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>MFF collapse of <i>wnt10a</i> mutant zebrafish is likely caused by the loss of distal properties in the developing MFF, strikingly similar to the proposed molecular pathomechanisms underlying the teeth defects caused by the loss of Wnt10 in fish and mammals. In addition, it points to thus fur unknown mechanisms controlling the linear growth and stability of actinotrichia and their collagen fibrils.</p>\n </section>\n </div>","PeriodicalId":11247,"journal":{"name":"Developmental Dynamics","volume":"253 6","pages":"566-592"},"PeriodicalIF":2.0000,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11035493/pdf/","citationCount":"0","resultStr":"{\"title\":\"wnt10a is required for zebrafish median fin fold maintenance and adult unpaired fin metamorphosis\",\"authors\":\"Erica L. Benard, Ismail Küçükaylak, Julia Hatzold, Kilian U. W. Berendes, Thomas J. Carney, Filippo Beleggia, Matthias Hammerschmidt\",\"doi\":\"10.1002/dvdy.672\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Background</h3>\\n \\n <p>Mutations of human <i>WNT10A</i> are associated with odonto-ectodermal dysplasia syndromes. Here, we present analyses of <i>wnt10a</i> loss-of-function mutants in the zebrafish.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p><i>wnt10a</i> mutant zebrafish embryos display impaired tooth development and a collapsing median fin fold (MFF). Rescue experiments show that <i>wnt10a</i> is essential for MFF maintenance both during embryogenesis and later metamorphosis. The MFF collapse could not be attributed to increased cell death or altered proliferation rates of MFF cell types. Rather, <i>wnt10a</i> mutants show reduced expression levels of <i>dlx2a</i> in distal-most MFF cells, followed by compromised expression of <i>col1a1a</i> and other extracellular matrix proteins encoding genes. Transmission electron microscopy analysis shows that although dermal MFF compartments of <i>wnt10a</i> mutants initially are of normal morphology, with regular collagenous actinotrichia, positioning of actinotrichia within the cleft of distal MFF cells becomes compromised, coinciding with actinotrichia shrinkage and MFF collapse.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusions</h3>\\n \\n <p>MFF collapse of <i>wnt10a</i> mutant zebrafish is likely caused by the loss of distal properties in the developing MFF, strikingly similar to the proposed molecular pathomechanisms underlying the teeth defects caused by the loss of Wnt10 in fish and mammals. In addition, it points to thus fur unknown mechanisms controlling the linear growth and stability of actinotrichia and their collagen fibrils.</p>\\n </section>\\n </div>\",\"PeriodicalId\":11247,\"journal\":{\"name\":\"Developmental Dynamics\",\"volume\":\"253 6\",\"pages\":\"566-592\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-10-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11035493/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Developmental Dynamics\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.672\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"ANATOMY & MORPHOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Developmental Dynamics","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/dvdy.672","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ANATOMY & MORPHOLOGY","Score":null,"Total":0}
wnt10a is required for zebrafish median fin fold maintenance and adult unpaired fin metamorphosis
Background
Mutations of human WNT10A are associated with odonto-ectodermal dysplasia syndromes. Here, we present analyses of wnt10a loss-of-function mutants in the zebrafish.
Results
wnt10a mutant zebrafish embryos display impaired tooth development and a collapsing median fin fold (MFF). Rescue experiments show that wnt10a is essential for MFF maintenance both during embryogenesis and later metamorphosis. The MFF collapse could not be attributed to increased cell death or altered proliferation rates of MFF cell types. Rather, wnt10a mutants show reduced expression levels of dlx2a in distal-most MFF cells, followed by compromised expression of col1a1a and other extracellular matrix proteins encoding genes. Transmission electron microscopy analysis shows that although dermal MFF compartments of wnt10a mutants initially are of normal morphology, with regular collagenous actinotrichia, positioning of actinotrichia within the cleft of distal MFF cells becomes compromised, coinciding with actinotrichia shrinkage and MFF collapse.
Conclusions
MFF collapse of wnt10a mutant zebrafish is likely caused by the loss of distal properties in the developing MFF, strikingly similar to the proposed molecular pathomechanisms underlying the teeth defects caused by the loss of Wnt10 in fish and mammals. In addition, it points to thus fur unknown mechanisms controlling the linear growth and stability of actinotrichia and their collagen fibrils.
期刊介绍:
Developmental Dynamics, is an official publication of the American Association for Anatomy. This peer reviewed journal provides an international forum for publishing novel discoveries, using any model system, that advances our understanding of development, morphology, form and function, evolution, disease, stem cells, repair and regeneration.