马氏珠母贝肉酪氨酸酶抑制肽的分离、鉴定和制备。

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
ACS Applied Bio Materials Pub Date : 2023-12-01 Epub Date: 2023-10-24 DOI:10.1007/s10529-023-03437-0
Jinhao Meng, Jiaojiao Liu, Jing Lu, Pingyingzi Jiang, Yunxia Bai, Xiaoling Liu, Shubo Li
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引用次数: 0

摘要

近年来,天然酪氨酸酶抑制剂在临床美容研究中受到重视。本研究利用地衣芽孢杆菌蛋白酶对马氏珠母贝肉进行酶解,经超滤和Sephadex G-15分离得到401个具有酪氨酸酶抑制作用的肽。利用分子对接技术评价肽对酪氨酸酶活性和结构的影响。选择结合能最低的三种合成肽W1(WDRPKDDGGSPIK)、W2(DRGYPPVMF)和W3(SGGGGGGGLGSGGSIRSSY)进行体外合成和生物活性研究。W3肽(5mg/mL)具有最高的酪氨酸酶活性、SPF、DPPH和ABTS清除值以及总抗氧化能力。W3不影响小鼠黑色素瘤B16-F10细胞的存活率(1.0-5.0mg/mL),但降低了黑色素含量。因此,W3可能适用于多功能酪氨酸酶抑制剂,并提供了一种利用海洋生物作为天然酪氨酸酶抑制剂来源的新方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Isolation, identification, and preparation of tyrosinase inhibitory peptides from Pinctada martensii meat.

Isolation, identification, and preparation of tyrosinase inhibitory peptides from Pinctada martensii meat.

Recently, natural tyrosinase inhibitors have gained attention in clinical cosmetology research. In this study, the enzymatic hydrolysis of Pinctada martensii meat by protease from Bacillus licheniformis, 401 peptides with tyrosinase inhibitory were identified after isolated by ultrafiltration and Sephadex G-15 from the fraction F4. The peptide effects on the tyrosinase activity and structure were evaluated using molecular docking. Three synthetic peptides classified as W1 (WDRPKDDGGSPIK), W2 (DRGYPPVMF), and W3 (SGGGGGGGLGSGGSIRSSY), which had the lowest binding energies were selected for in vitro synthesis and biological activity investigation. The W3 peptide (5 mg/mL) had the highest tyrosinase activity, SPF, DPPH, and ABTS clearance values, and total antioxidant capacity. W3 did not affect the survival rate of mouse melanoma B16-F10 cells (1.0-5.0 mg/mL) but decreased the melanin content. Hence, W3 could be suitable for multifunctional tyrosinase inhibition and provides a novel method to use marine organisms as natural tyrosinase inhibitor sources.

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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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