Lev A. Dykman, Sergey A. Staroverov, Pavel V. Mezhenny, Alexander S. Fomin, Sergey V. Kozlov, Alexey A. Volkov, Vladislav N. Laskavy, Sergei Yu. Shchyogolev
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Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response
Foot-and-mouth disease is an acute, highly contagious infection of domestic and wild cloven-hoofed animals, which can be transmitted to humans. In many cases, the existing vaccines are not quite effective. The purpose of this study was to test the possibility of using gold nanoparticles as an antigen carrier and an adjuvant. The immunogenic properties of gold nanoparticles were assessed by conjugating the particles to a synthetic peptide of the VP1 capsid protein of the foot-and-mouth disease virus. The resulting conjugate (with or without the use of complete Freund’s adjuvant), a commercial vaccine, and the native peptide served to immunize guinea pigs. The titer and sensitivity of the raised antibodies were maximal for the combination comprising the nanoparticle–peptide conjugate and complete Freund’s adjuvant. Antibody biosynthesis was accompanied by increased production of proinflammatory cytokines (especially IFN-γ) and by stimulation of the respiratory activity of peritoneal macrophages. The use of gold nanoparticles as a hapten carrier enhanced the immune response even when complete Freund’s adjuvant was not used.
期刊介绍:
Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.