利用合成的口蹄疫病毒肽偶联金纳米颗粒增强免疫反应

IF 2.2 4区 工程技术 Q2 Chemistry
Lev A. Dykman, Sergey A. Staroverov, Pavel V. Mezhenny, Alexander S. Fomin, Sergey V. Kozlov, Alexey A. Volkov, Vladislav N. Laskavy, Sergei Yu. Shchyogolev
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引用次数: 19

摘要

口蹄疫是家畜和野生偶蹄动物的一种急性、高度传染性传染病,可传染给人类。在许多情况下,现有的疫苗不是很有效。本研究的目的是测试使用金纳米颗粒作为抗原载体和佐剂的可能性。通过将金纳米颗粒与口蹄疫病毒VP1衣壳蛋白的合成肽偶联,评估了金纳米颗粒的免疫原性。所得到的结合物(有或没有使用完全的弗氏佐剂)、一种商业疫苗和天然肽用于免疫豚鼠。当纳米颗粒-肽偶联物与完全的弗氏佐剂结合时,抗体的效价和敏感性最高。抗体的生物合成伴随着促炎细胞因子(尤其是IFN-γ)的增加和腹腔巨噬细胞呼吸活性的刺激。即使不使用完全的弗氏佐剂,使用金纳米颗粒作为半抗原载体也能增强免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Use of a synthetic foot-and-mouth disease virus peptide conjugated to gold nanoparticles for enhancing immunological response

Foot-and-mouth disease is an acute, highly contagious infection of domestic and wild cloven-hoofed animals, which can be transmitted to humans. In many cases, the existing vaccines are not quite effective. The purpose of this study was to test the possibility of using gold nanoparticles as an antigen carrier and an adjuvant. The immunogenic properties of gold nanoparticles were assessed by conjugating the particles to a synthetic peptide of the VP1 capsid protein of the foot-and-mouth disease virus. The resulting conjugate (with or without the use of complete Freund’s adjuvant), a commercial vaccine, and the native peptide served to immunize guinea pigs. The titer and sensitivity of the raised antibodies were maximal for the combination comprising the nanoparticle–peptide conjugate and complete Freund’s adjuvant. Antibody biosynthesis was accompanied by increased production of proinflammatory cytokines (especially IFN-γ) and by stimulation of the respiratory activity of peritoneal macrophages. The use of gold nanoparticles as a hapten carrier enhanced the immune response even when complete Freund’s adjuvant was not used.

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来源期刊
Gold Bulletin
Gold Bulletin 工程技术-材料科学:综合
CiteScore
3.30
自引率
4.50%
发文量
0
审稿时长
3 months
期刊介绍: Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.
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