Michael Josef Kridanto Kamadjaja, Janery Fidelia Abraham, Harry Laksono
{"title":"Pelagicus羟基磷灰石移植体对人牙龈成纤维细胞培养的生物相容性。","authors":"Michael Josef Kridanto Kamadjaja, Janery Fidelia Abraham, Harry Laksono","doi":"10.5455/medarh.2019.73.303-306","DOIUrl":null,"url":null,"abstract":"<p><strong>Introduction: </strong>Crab shell (<i>Portunus pelagicus</i>) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast.</p><p><strong>Aim: </strong>This study aimed to determine the biocompatibility of <i>Portunus pelagicus</i> hydroxyapatite graft on human gingival fibroblast cell culture.</p><p><strong>Material and methods: </strong>Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from <i>Portunus pelagicus</i> at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova.</p><p><strong>Results: </strong>The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group.</p><p><strong>Conclusion: </strong>The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.</p>","PeriodicalId":94135,"journal":{"name":"Medical archives (Sarajevo, Bosnia and Herzegovina)","volume":"73 5","pages":"303-306"},"PeriodicalIF":0.0000,"publicationDate":"2019-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/3f/medarch-73-303.PMC6885209.pdf","citationCount":"3","resultStr":"{\"title\":\"Biocompatibility of Portunus Pelagicus Hydroxyapatite Graft on Human Gingival Fibroblast Cell Culture.\",\"authors\":\"Michael Josef Kridanto Kamadjaja, Janery Fidelia Abraham, Harry Laksono\",\"doi\":\"10.5455/medarh.2019.73.303-306\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Introduction: </strong>Crab shell (<i>Portunus pelagicus</i>) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast.</p><p><strong>Aim: </strong>This study aimed to determine the biocompatibility of <i>Portunus pelagicus</i> hydroxyapatite graft on human gingival fibroblast cell culture.</p><p><strong>Material and methods: </strong>Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from <i>Portunus pelagicus</i> at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova.</p><p><strong>Results: </strong>The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group.</p><p><strong>Conclusion: </strong>The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.</p>\",\"PeriodicalId\":94135,\"journal\":{\"name\":\"Medical archives (Sarajevo, Bosnia and Herzegovina)\",\"volume\":\"73 5\",\"pages\":\"303-306\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/ff/3f/medarch-73-303.PMC6885209.pdf\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Medical archives (Sarajevo, Bosnia and Herzegovina)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5455/medarh.2019.73.303-306\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medical archives (Sarajevo, Bosnia and Herzegovina)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5455/medarh.2019.73.303-306","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Biocompatibility of Portunus Pelagicus Hydroxyapatite Graft on Human Gingival Fibroblast Cell Culture.
Introduction: Crab shell (Portunus pelagicus) has the potential to be a source of hydroxyapatite biomaterials that used as bone grafts. Before clinical application, crab shell graft should be tested for its biocompatibility in vitro on human gingival fibroblast.
Aim: This study aimed to determine the biocompatibility of Portunus pelagicus hydroxyapatite graft on human gingival fibroblast cell culture.
Material and methods: Human gingival fibroblast cell cultures were divided into control group and treatment group with the addition of hydroxyapatite graft powder from Portunus pelagicus at a concentration of 100 ppm, 50 ppm, and 25 ppm. The synthesis process of hydroxyapatite was conducted by heating at 1000°C then characterizing the compound with SEM-EDX. All samples were incubated in α-MEM medium, then were given MTT material. The cultures on the plate were examined using ELISA reader. The results were analyzed using a Oneway Anova.
Results: The percentage of living cells throughout all treatment group shown results that exceeded the LD50 parameter. The highest percentage of living cells was at 25 ppm concentration group.
Conclusion: The hydroxyapatite graft powder from crab shells is biocompatible with human gingival fibroblast cell culture.
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