Shweta Kumari, Peter John, Ahila Singaravel Chidambaranathan, Balasubramanium Muthukumar
{"title":"不同浓度的钛和银纳米颗粒对颌面部有机硅MDX4-4210细胞活力、撕裂强度和岸强度的影响：一项体外研究。","authors":"Shweta Kumari, Peter John, Ahila Singaravel Chidambaranathan, Balasubramanium Muthukumar","doi":"10.4103/jips.jips_378_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>The aim was to evaluate and compare the cell viability, tear bond strength, and shore hardness of MDX4-4210 silicone reinforced with titanium dioxide and silver nanoparticles in 1%, 2%, and 3%.</p><p><strong>Settings and design: </strong>The study design involves in vitro comparative study.</p><p><strong>Materials and methods: </strong>MDX4-4210 silicone incorporated with 1%, 2%, and 3% by weight of silver and titanium dioxide nanoparticles. A total of 112 specimens were prepared and grouped into 7, with 16 specimens in each group. Group 1 - control, Groups 2, 3, and 4 were 1%, 2%, and 3% silver, respectively. Groups 5, 6, and 7 were 1%, 2%, and 3% titanium dioxide nanoparticles, respectively. Cell viability was tested by MTT ASSAY on MG63 cell lines, tear bond strength was tested by peeling force in universal testing machine, and Shore A hardness was tested in durometer.</p><p><strong>Statistical analysis used: </strong>The cell viability values were statistically analysed using one-way analysis of variance, and Tukey honestly significant difference test, tear bond strength and shore hardness values were analysed using Mann-Whitney test.</p><p><strong>Results: </strong>Based on the MTT ASSAY test, 1% silver nanoparticles incorporated MDX4-4210 silicone showed maximum cell viability of 42.10%, whereas minimum cell viability was 18.06% for 3% of titanium dioxide-reinforced silicone. The mean value of tear bond strength of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 62.81 ± 3.637 N/m and 59.69 ± 5.313 N/m and the mean value of shore hardness of room temperature vulcanized of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 38.06 ± 1.237 and 36.75 ± 1.291.</p><p><strong>Conclusion: </strong>Cell viability of 1% silver nanoparticles reinforced MDX4-4210 silicone was higher in comparison to the other groups, and tear bond strength and shore hardness were significantly higher in 1% silver nanoparticles reinforced silicone compared to 1% titanium nanoparticles reinforced MDX4-4210 silicone.</p>","PeriodicalId":22669,"journal":{"name":"The Journal of Indian Prosthodontic Society","volume":"23 4","pages":"384-390"},"PeriodicalIF":1.0000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10705015/pdf/","citationCount":"0","resultStr":"{\"title\":\"Effect of different concentrations of titanium and silver nanoparticles on maxillofacial silicone MDX4-4210 on cell viability, tear bond strength, and shore strength: An <i>in vitro</i> study.\",\"authors\":\"Shweta Kumari, Peter John, Ahila Singaravel Chidambaranathan, Balasubramanium Muthukumar\",\"doi\":\"10.4103/jips.jips_378_23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>The aim was to evaluate and compare the cell viability, tear bond strength, and shore hardness of MDX4-4210 silicone reinforced with titanium dioxide and silver nanoparticles in 1%, 2%, and 3%.</p><p><strong>Settings and design: </strong>The study design involves in vitro comparative study.</p><p><strong>Materials and methods: </strong>MDX4-4210 silicone incorporated with 1%, 2%, and 3% by weight of silver and titanium dioxide nanoparticles. A total of 112 specimens were prepared and grouped into 7, with 16 specimens in each group. Group 1 - control, Groups 2, 3, and 4 were 1%, 2%, and 3% silver, respectively. Groups 5, 6, and 7 were 1%, 2%, and 3% titanium dioxide nanoparticles, respectively. Cell viability was tested by MTT ASSAY on MG63 cell lines, tear bond strength was tested by peeling force in universal testing machine, and Shore A hardness was tested in durometer.</p><p><strong>Statistical analysis used: </strong>The cell viability values were statistically analysed using one-way analysis of variance, and Tukey honestly significant difference test, tear bond strength and shore hardness values were analysed using Mann-Whitney test.</p><p><strong>Results: </strong>Based on the MTT ASSAY test, 1% silver nanoparticles incorporated MDX4-4210 silicone showed maximum cell viability of 42.10%, whereas minimum cell viability was 18.06% for 3% of titanium dioxide-reinforced silicone. The mean value of tear bond strength of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 62.81 ± 3.637 N/m and 59.69 ± 5.313 N/m and the mean value of shore hardness of room temperature vulcanized of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 38.06 ± 1.237 and 36.75 ± 1.291.</p><p><strong>Conclusion: </strong>Cell viability of 1% silver nanoparticles reinforced MDX4-4210 silicone was higher in comparison to the other groups, and tear bond strength and shore hardness were significantly higher in 1% silver nanoparticles reinforced silicone compared to 1% titanium nanoparticles reinforced MDX4-4210 silicone.</p>\",\"PeriodicalId\":22669,\"journal\":{\"name\":\"The Journal of Indian Prosthodontic Society\",\"volume\":\"23 4\",\"pages\":\"384-390\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10705015/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Indian Prosthodontic Society\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jips.jips_378_23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"DENTISTRY, ORAL SURGERY & MEDICINE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Indian Prosthodontic Society","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jips.jips_378_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}
Effect of different concentrations of titanium and silver nanoparticles on maxillofacial silicone MDX4-4210 on cell viability, tear bond strength, and shore strength: An in vitro study.
Aim: The aim was to evaluate and compare the cell viability, tear bond strength, and shore hardness of MDX4-4210 silicone reinforced with titanium dioxide and silver nanoparticles in 1%, 2%, and 3%.
Settings and design: The study design involves in vitro comparative study.
Materials and methods: MDX4-4210 silicone incorporated with 1%, 2%, and 3% by weight of silver and titanium dioxide nanoparticles. A total of 112 specimens were prepared and grouped into 7, with 16 specimens in each group. Group 1 - control, Groups 2, 3, and 4 were 1%, 2%, and 3% silver, respectively. Groups 5, 6, and 7 were 1%, 2%, and 3% titanium dioxide nanoparticles, respectively. Cell viability was tested by MTT ASSAY on MG63 cell lines, tear bond strength was tested by peeling force in universal testing machine, and Shore A hardness was tested in durometer.
Statistical analysis used: The cell viability values were statistically analysed using one-way analysis of variance, and Tukey honestly significant difference test, tear bond strength and shore hardness values were analysed using Mann-Whitney test.
Results: Based on the MTT ASSAY test, 1% silver nanoparticles incorporated MDX4-4210 silicone showed maximum cell viability of 42.10%, whereas minimum cell viability was 18.06% for 3% of titanium dioxide-reinforced silicone. The mean value of tear bond strength of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 62.81 ± 3.637 N/m and 59.69 ± 5.313 N/m and the mean value of shore hardness of room temperature vulcanized of 1% silver and 1% titanium dioxide nanoparticles reinforced room temperature vulcanized maxillofacial silicone were 38.06 ± 1.237 and 36.75 ± 1.291.
Conclusion: Cell viability of 1% silver nanoparticles reinforced MDX4-4210 silicone was higher in comparison to the other groups, and tear bond strength and shore hardness were significantly higher in 1% silver nanoparticles reinforced silicone compared to 1% titanium nanoparticles reinforced MDX4-4210 silicone.
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