毕赤酵母制备大豆血红蛋白蛋白的安全性:体外和体内研究,毕赤酵母表达大豆血红蛋白基因。

IF 3.4 Q2 TOXICOLOGY
Journal of Toxicology Pub Date : 2023-10-10 eCollection Date: 2023-01-01 DOI:10.1155/2023/7398724
Teresa F Reyes, Puja Agrawal, Teresa Chan, Richard Green, Ray A Matulka
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引用次数: 0

摘要

大豆血红蛋白(LegH)蛋白来源于毕赤酵母(Pichia pastoris,重新分类为Komagataella phaffii)生产的大豆(Glycine max)作为LegH Prep,是一种新型食品成分,为植物衍生食品提供类似肉类的风味和香气。LegH Prep的安全性之前已经在一系列体内和体外测试中进行了评估,在测试条件下没有发现不良反应。在这项新的工作中,我们介绍了评估LegH Prep安全性的新的体内和体外试验结果。LegH Prep在细菌反向突变试验中是非致突变的,在人类淋巴细胞的体外微核试验中是无成纤维细胞的。在90 雄性和雌性Sprague-Dawley®大鼠的一天饮食研究,包括一项28 天恢复期。该研究没有导致与最高剂量LegH Prep给药相关的动物死亡(90000 ppm)。在28个疗程中,LegH Prep给药没有引起显著的临床或生理不良变化,也没有观察到对雄性或雌性大鼠的不良影响 日间恢复阶段研究。新90 日粮毒性研究确定了4798.3和5761.5的无不良反应水平(NOAEL) mg/kg/天,分别为雄性和雌性大鼠测试的最高水平。因此,研究结果表明,在测试条件下,LegH Prep对肉类类似产品的食用无毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

The Safety of Soy Leghemoglobin Protein Preparation Derived from <i>Pichia pastoris</i> Expressing a Soy Leghemoglobin Gene from <i>Glycine max</i>: <i>In Vitro</i> and <i>In Vivo</i> Studies.

The Safety of Soy Leghemoglobin Protein Preparation Derived from Pichia pastoris Expressing a Soy Leghemoglobin Gene from Glycine max: In Vitro and In Vivo Studies.

Soy leghemoglobin (LegH) protein derived from soy (Glycine max) produced in Pichia pastoris (reclassified as Komagataella phaffii) as LegH Prep is a novel food ingredient that provides meat-like flavor and aroma to plant-derived food products. The safety of LegH Prep has been previously assessed in a battery of in vivo and in vitro testing and found no adverse effects under the conditions tested. In this new work, we present the results of new in vivo and in vitro tests evaluating the safety of LegH Prep. LegH Prep was nonmutagenic in a bacterial reverse mutation assay and nonclastogenic in an in vitro micronucleus assay in human lymphocytes. Systemic toxicity was evaluated in the 90 day dietary study in male and female Sprague-Dawley® rats that included a 28 day recovery period. The study resulted in no animal deaths associated with the administration of LegH Prep at the highest dose (90,000 ppm). There were no significant adverse clinical or physical changes attributed to LegH Prep administration, and no observed adverse effects on either male or female rats over the course of the 28 day recovery phase study. The new 90 day dietary toxicity study established a no observed adverse effect level (NOAEL) of 4798.3 and 5761.5 mg/kg/day, the maximum level tested for male and female rats, respectively. Thus, the results of the studies demonstrate that under the conditions tested, LegH Prep is not toxic for consumption in meat analog products.

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来源期刊
Journal of Toxicology
Journal of Toxicology TOXICOLOGY-
CiteScore
5.50
自引率
3.40%
发文量
0
审稿时长
10 weeks
期刊介绍: Journal of Toxicology is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies in all areas of toxicological sciences. The journal will consider articles looking at the structure, function, and mechanism of agents that are toxic to humans and/or animals, as well as toxicological medicine, risk assessment, safety evaluation, and environmental health.
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