Ana B Vargas-Antillón, Mildren Porchas-Quijada, Eloy A Zepeda-Carrillo, Rafael Torres-Valadez, José F Muñoz-Valle, Rafael Vázquez-Solórzano, Elia Valdés-Miramontes, Luis A Hernández-Palma, Zyanya Reyes-Castillo
{"title":"成人2型糖尿病患者对瘦素反应的抗体及其与临床、代谢和心血管风险参数的关系。","authors":"Ana B Vargas-Antillón, Mildren Porchas-Quijada, Eloy A Zepeda-Carrillo, Rafael Torres-Valadez, José F Muñoz-Valle, Rafael Vázquez-Solórzano, Elia Valdés-Miramontes, Luis A Hernández-Palma, Zyanya Reyes-Castillo","doi":"10.1080/07435800.2023.2270763","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and aims: </strong>Patients with obesity and type 2 diabetes (T2D) have shown alterations in the affinity of IgG anti-leptin antibodies which are possibly related to metabolic alterations. In the present exploratory study, we analyzed serum samples from adults with T2D classified by body mass index (BMI) and evaluated the relationship of IgG anti-leptin antibodies with body composition, metabolic and cardiovascular risk parameters.</p><p><strong>Methods: </strong>Serum IgG anti-leptin antibodies (total, free and immune complexes fractions) were measured by in-house ELISA. Body composition, metabolic biomarkers (glucose, glycated hemoglobin, lipid profile, insulin, leptin) and cardiometabolic risk indexes (AIP, HOMA-IR, HOMA-ß) were evaluated in one hundred T2D patients.</p><p><strong>Results: </strong>Patients with T2D and obesity presented a decrease in the percentage of IgG anti-leptin immune complexes compared to patients with T2D and overweight (<i>p</i> < 0.0053). Negative correlations of IgG anti-leptin immune complexes with triglycerides (TG) (<i>r</i>=-0.412, <i>p</i> = 0.023) and VLDL-C (<i>r</i>=-0.611, <i>p</i> = 0.017) were found in normal weight T2D patients. Free IgG anti-leptin antibodies correlated positively with TC (<i>r</i> = 0.390, <i>p</i> = 0.032) and LDL-C (<i>r</i> = 0.458, <i>p</i> = 0.011) in overweight individuals with T2D. Finally, total IgG anti-leptin antibodies correlated positively with leptin hormone levels (<i>r</i> = 0.409, <i>p</i> = 0.024) and negatively with HOMA-IR (<i>r</i> =-0.459, <i>p</i> = 0.012) in T2D patients with obesity.</p><p><strong>Conclusions: </strong>The decrease of IgG anti-leptin immune complexes observed in patients with T2D and obesity suggests a reduction in antibody affinity to the hormone that may impact its transport and signaling, lipid, lipoprotein and insulin metabolism.</p>","PeriodicalId":11601,"journal":{"name":"Endocrine Research","volume":" ","pages":"12-21"},"PeriodicalIF":1.5000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Antibodies Reactive to Leptin in Adults with Type 2 Diabetes and Its Relationship with Clinical, Metabolic and Cardiovascular Risk Parameters.\",\"authors\":\"Ana B Vargas-Antillón, Mildren Porchas-Quijada, Eloy A Zepeda-Carrillo, Rafael Torres-Valadez, José F Muñoz-Valle, Rafael Vázquez-Solórzano, Elia Valdés-Miramontes, Luis A Hernández-Palma, Zyanya Reyes-Castillo\",\"doi\":\"10.1080/07435800.2023.2270763\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and aims: </strong>Patients with obesity and type 2 diabetes (T2D) have shown alterations in the affinity of IgG anti-leptin antibodies which are possibly related to metabolic alterations. In the present exploratory study, we analyzed serum samples from adults with T2D classified by body mass index (BMI) and evaluated the relationship of IgG anti-leptin antibodies with body composition, metabolic and cardiovascular risk parameters.</p><p><strong>Methods: </strong>Serum IgG anti-leptin antibodies (total, free and immune complexes fractions) were measured by in-house ELISA. Body composition, metabolic biomarkers (glucose, glycated hemoglobin, lipid profile, insulin, leptin) and cardiometabolic risk indexes (AIP, HOMA-IR, HOMA-ß) were evaluated in one hundred T2D patients.</p><p><strong>Results: </strong>Patients with T2D and obesity presented a decrease in the percentage of IgG anti-leptin immune complexes compared to patients with T2D and overweight (<i>p</i> < 0.0053). Negative correlations of IgG anti-leptin immune complexes with triglycerides (TG) (<i>r</i>=-0.412, <i>p</i> = 0.023) and VLDL-C (<i>r</i>=-0.611, <i>p</i> = 0.017) were found in normal weight T2D patients. Free IgG anti-leptin antibodies correlated positively with TC (<i>r</i> = 0.390, <i>p</i> = 0.032) and LDL-C (<i>r</i> = 0.458, <i>p</i> = 0.011) in overweight individuals with T2D. Finally, total IgG anti-leptin antibodies correlated positively with leptin hormone levels (<i>r</i> = 0.409, <i>p</i> = 0.024) and negatively with HOMA-IR (<i>r</i> =-0.459, <i>p</i> = 0.012) in T2D patients with obesity.</p><p><strong>Conclusions: </strong>The decrease of IgG anti-leptin immune complexes observed in patients with T2D and obesity suggests a reduction in antibody affinity to the hormone that may impact its transport and signaling, lipid, lipoprotein and insulin metabolism.</p>\",\"PeriodicalId\":11601,\"journal\":{\"name\":\"Endocrine Research\",\"volume\":\" \",\"pages\":\"12-21\"},\"PeriodicalIF\":1.5000,\"publicationDate\":\"2024-01-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Endocrine Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/07435800.2023.2270763\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/1/4 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q4\",\"JCRName\":\"ENDOCRINOLOGY & METABOLISM\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Endocrine Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/07435800.2023.2270763","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/4 0:00:00","PubModel":"Epub","JCR":"Q4","JCRName":"ENDOCRINOLOGY & METABOLISM","Score":null,"Total":0}
Antibodies Reactive to Leptin in Adults with Type 2 Diabetes and Its Relationship with Clinical, Metabolic and Cardiovascular Risk Parameters.
Background and aims: Patients with obesity and type 2 diabetes (T2D) have shown alterations in the affinity of IgG anti-leptin antibodies which are possibly related to metabolic alterations. In the present exploratory study, we analyzed serum samples from adults with T2D classified by body mass index (BMI) and evaluated the relationship of IgG anti-leptin antibodies with body composition, metabolic and cardiovascular risk parameters.
Methods: Serum IgG anti-leptin antibodies (total, free and immune complexes fractions) were measured by in-house ELISA. Body composition, metabolic biomarkers (glucose, glycated hemoglobin, lipid profile, insulin, leptin) and cardiometabolic risk indexes (AIP, HOMA-IR, HOMA-ß) were evaluated in one hundred T2D patients.
Results: Patients with T2D and obesity presented a decrease in the percentage of IgG anti-leptin immune complexes compared to patients with T2D and overweight (p < 0.0053). Negative correlations of IgG anti-leptin immune complexes with triglycerides (TG) (r=-0.412, p = 0.023) and VLDL-C (r=-0.611, p = 0.017) were found in normal weight T2D patients. Free IgG anti-leptin antibodies correlated positively with TC (r = 0.390, p = 0.032) and LDL-C (r = 0.458, p = 0.011) in overweight individuals with T2D. Finally, total IgG anti-leptin antibodies correlated positively with leptin hormone levels (r = 0.409, p = 0.024) and negatively with HOMA-IR (r =-0.459, p = 0.012) in T2D patients with obesity.
Conclusions: The decrease of IgG anti-leptin immune complexes observed in patients with T2D and obesity suggests a reduction in antibody affinity to the hormone that may impact its transport and signaling, lipid, lipoprotein and insulin metabolism.
期刊介绍：
This journal publishes original articles relating to endocrinology in the broadest context. Subjects of interest include: receptors and mechanism of action of hormones, methodological advances in the detection and measurement of hormones; structure and chemical properties of hormones. Invitations to submit Brief Reviews are issued to specific authors by the Editors.
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